Isolation of microorganisms capable of synthesising amino acids, utilizing hydrocarbons, has been reported. These microorganisms were isolated from soil samples by selective culture techniques. 91 strains were found capable of producing amino acids in the broth. Different amino acids and their maximum yield obtained were glutamic acid 160mg/l; leucine 90.0mg/l; isoleucine 40.0mg/1; valine 105.0mg/l; methionine 25.0mg/l; tryptophan 2.5 mg/l; arginine 70.0mg/l; and histidine 10.0mg/l.