著者

Bingyi Liang Hikaru Sato Sachihiro Matsunaga

出版者

Japan Mendel Society, International Society of Cytology

雑誌

CYTOLOGIA (ISSN:00114545)

巻号頁・発行日

vol.88, no.4, pp.283-288, 2023-12-25 (Released:2023-12-25)

参考文献数

59

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Gene editing technology based on the clustered regularly interspaced short palindromic repeats (CRISPR) system has garnered widespread use in plant genomes because of its cost-effectiveness, efficiency, and simplicity. To avoid the integration of foreign genes and any DNA fragments into target cell genomes, researchers have developed a system that introduces in vitro-assembled ribonucleoproteins (RNPs) consisting of guide RNA (gRNA) and Cas protein into target cells, enabling direct genome editing. This system was designed to deliver RNPs through four distinct methods: polyethylene glycol (PEG)-mediated cell transfection, particle bombardment, electroporation, and lipid transfection. In recent years, CRISPR technology has been extensively applied for the genetic modification of plants, providing a strategic response to environmental challenges. Researchers have successfully established RNP genome editing systems in various plant species. Despite some remaining issues, the RNP genome editing system still shows significant promise for future applications in the production of non-genetically modified (non-GM) crops.

著者

Junko Hasegawa Takumi Higaki Yuki Hamamura Daisuke Kurihara Natsumaro Kutsuna Tetsuya Higashiyama Seiichiro Hasezawa Sachihiro Matsunaga

出版者

日本メンデル協会、国際細胞学会

雑誌

CYTOLOGIA (ISSN:00114545)

巻号頁・発行日

vol.79, no.4, pp.467-474, 2014-12-25 (Released:2015-01-16)

参考文献数

31

被引用文献数

1 7

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Vacuoles occupy 80–90% of a mature plant cell and mainly contribute to all types of cell expansion. Zeocin, an inducer of DNA double-strand breaks, causes cell expansion with endoreduplication. The vacuolar structure after zeocin treatment was examined in tobacco (Nicotiana tabacum) cultured cells expressing GFP fused to a vacuole membrane protein. We found that the genotoxic stress induced the cell expansion with subdivision of the vacuolar lumen by cytoplasmic strands. When a femtosecond laser was used to cut off the cytoplasmic strand, mitochondrial transport along the strand stopped. This suggested that in the elongated cells under the genotoxic stress, the transport of subcellular materials was activated for DNA repair within the damaged cell nucleus by the construction of a network of cytoplasmic strands in the vacuolar lumen.

著者

Yoji Okabe Sachihiro Matsunaga

出版者

Japan Mendel Society, International Society of Cytology

雑誌

CYTOLOGIA (ISSN:00114545)

巻号頁・発行日

vol.87, no.2, pp.69-72, 2022-06-25 (Released:2022-06-25)

参考文献数

36

被引用文献数

1

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Photosymbiosis occurs in symbiotic relationships between heterotrophs and photosymbiotic organisms, where a photosynthetic symbiont provides photosynthate to a host. While almost all known host animals are invertebrates, experiments have determined that artificial symbiotic relationships can be established between vertebrates and phototrophs. The ability to generate photosymbiotic relationships in vertebrates has important applications for medical treatments and commercial resource production.

著者

Satoru Fujimoto Sachihiro Matsunaga

出版者

Japan Mendel Society, International Society of Cytology

雑誌

CYTOLOGIA (ISSN:00114545)

巻号頁・発行日

vol.82, no.5, pp.559-562, 2017-12-25 (Released:2018-01-24)

参考文献数

28

被引用文献数

15

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Labeling of specific DNA sequences in living organisms is important for understanding the mechanism of gene regulation during development, differentiation, and environmental adaptation. Here we describe a CRISPR/Cas9-based chromatin visualization system in plants. We adapted the mammalian nuclease-dead Cas9-fluorescent protein (dCas9-FP) system to a plant expression vector. Transient expression of dCas9-FP and single guide RNA (sgRNA) for telomeric sequences efficiently labeled telomere repeats in tobacco.

著者

Sachihiro Matsunaga

出版者

The Genetics Society of Japan

雑誌

Genes & Genetic Systems (ISSN:13417568)

巻号頁・発行日

vol.81, no.4, pp.219-226, 2006 (Released:2006-10-11)

参考文献数

78

被引用文献数

16 22

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Recent studies of plant sex chromosome-linked genes have revealed many interesting characteristics, although there are limited reports about heteromorphic sex chromosomes in flowering plants. Sex chromosome-linked genes in angiosperms have been characterized mainly in the dioecious plant Silene latifolia. Although all such genes were isolated from transcripts of male flower buds of S. latifolia, most seem to be housekeeping genes except for the petal- and stamen-specific MADS box gene on the Y chromosome (SlAP3Y) and the male reproductive organ-specific gene on the X chromosome (MROS3X). Recent evolutionary studies have revealed at least three evolutionary strata on the X chromosome that are related to stepwise loss of recombination between the sex chromosomes. Moreover, genetic maps showed conservation of gene organization on the X chromosome in the genus Silene and substantial pericentric inversion between the X and Y chromosomes of S. latifolia during evolution. A comparison between paralogs on the sex chromosomes revealed that introns of the Y-linked genes are longer than those of X-linked paralogs. Although analyses of sex chromosome-linked genes suggest that degeneration of the Y chromosome has occurred, the Y chromosome in flowering plants remains the largest in the male genome, unlike that of mammals. Accumulation of repetitive sequences and the entire chloroplast genome on the Y chromosome appear to have contributed to this large size. However, more detailed studies will be required to help explain the basis for the fact that heteromorphic sex chromosomes in angiosperms are large.