著者
Shamimul Alam Sheikh Katsuhiko Kondo Yoshikazu Hoshi
出版者
日本メンデル協会、国際細胞学会
雑誌
CYTOLOGIA (ISSN:00114545)
巻号頁・発行日
vol.60, no.1, pp.43-47, 1995-03-25 (Released:2009-03-19)
参考文献数
11
被引用文献数
24 40

Drosera dichrosepala was exposed to different doses of Gamma radiation. Fragmented and fused chromosomes were observed as a consequences. Diffused centromeres in every fragment chromosome was detected by centromeric banding (Cd-banding) and was supported by its typical disjunction and totally lack of lagging chromosomes or micronuclei from anaphase to telophase. C-banding revealed that the fragment chromosomes were highly heterochromatic and fragmentation might be occurred at the terminal regions of chromosomes. Fluorescent banding suggested that most of the fragment chromosomes were rich in GC base composition in the species. Alteration of karyotype due to Gamma irradiation also indicated that spontaneous fragmentation or fusion of chromosomes might be a possible factor for promoting the bimodal karyotype in this genus.
著者
Yumi Hirakawa Seiichiro Hasezawa Takumi Higaki
出版者
Japan Mendel Society, International Society of Cytology
雑誌
CYTOLOGIA (ISSN:00114545)
巻号頁・発行日
vol.83, no.3, pp.289-293, 2018-09-25 (Released:2018-10-12)
参考文献数
29

We previously established an experimental system for efficient pathogenic signal induced-cell death using tobacco BY-2 cells and culture filtrate of a plant pathogenic bacterium, Erwinia carotovora. Using this experimental system, cytoskeletal and vacuolar changes during the process of filtrate-induced cell death were characterized in detail. However, the initial events induced by the filtrate were largely unknown. In this study, reactive oxygen species (ROS) production and endocytosis stimulation were examined in BY-2 cells treated with the filtrate of E. carotovora. Transient ROS production within 2 min was observed after the filtrate treatment. Treatment with the Ca2+ chelator BAPTA or the protein kinase inhibitor K252a significantly inhibited ROS production in a dose-dependent manner, suggesting that ROS production depends on Ca2+ influx or protein phosphorylation. In addition, internalization of the endocytic marker FM4-64 was promoted by filtrate treatment within 30 min, suggesting that the filtrate treatment stimulated endocytosis. These results showed that ROS production and subsequent endocytosis stimulation are the initial events induced by culture filtrate of E. carotovora in BY-2 cells.
著者
Mina Ohtsu Daisuke Kurihara Yoshikatsu Sato Takuya Suzaki Masayoshi Kawaguchi Daisuke Maruyama Tetsuya Higashiyama
出版者
日本メンデル協会、国際細胞学会
雑誌
CYTOLOGIA (ISSN:00114545)
巻号頁・発行日
vol.82, no.3, pp.251-259, 2017-06-25 (Released:2017-09-06)
参考文献数
35

Nematode infection of plant roots is a paradigm of host–parasite interactions. Although nematodes can be labeled with fluorescent dyes, migration of the worms into the deep regions of host roots makes them difficult to track. Here we report the use of two fluorescent dyes, FM4-64 and SYBR green I, to intensely label the soybean cyst nematode (SCN) Heterodera glycines for one week in host plants. Continuous monitoring of the labeled SCN juveniles was achieved with two-photon microscopy. Additionally, we developed a transient transformation system consisting of the non-model leguminous plant (fabaceous) roots, Astragalus sinicus and Agrobacterium rhizogenes to observe the cellular structures of the plant during SCN infection. By the combined use of fluorescent dyes and two-photon microscopy, clear images of infecting SCNs were obtained even in deep regions of A. sinicus roots. The fluorescent labeling described herein can also be used in detailed monitoring of the infection processes of other non-model nematodes, as well as the associated morphological changes in the host plant roots.
著者
Daniel Luis Zanella Kantek Alberto Sergio Fenocchio Marta Margarete Cestari
出版者
日本メンデル協会、国際細胞学会
雑誌
CYTOLOGIA (ISSN:00114545)
巻号頁・発行日
vol.68, no.1, pp.19-24, 2003 (Released:2003-10-10)
参考文献数
28
被引用文献数
1 4

The characids belong to the greater freshwater fish family of the world with around 700 recognized species. Whithin this group the genus Astyanax includes several species with unclear phylogenetic relationships, showing a wide karyotypic diversity. In the highly endemic Iguaçu river basin these problems are also observed being distinguished 6 unnamed Astyanax species (A to F). In the present work were cytogenetically studied 2 populations of A. sp. C from the first plateau of the Iguaçu river (Paraná State, Brazil). Fishes of both populations are similar in chromosome number, having 2n=50, however were evidenced clear differences in C-banding and nucleolar organizing regions (NORs) patterns. With the use of the restriction enzyme Alu I were obtained the same results as C-bands. These results indicate the complexity of Astyanax and the importance of further taxonomic revisions joining morphological, cytogenetic and molecular approachings.
著者
Lana Cristina Fazoli Valquíria de Almeida Bernardo da Silva Ana Luiza de Brito Portela-Castro Horácio Ferreira Júlio Júnior
出版者
日本メンデル協会、国際細胞学会
雑誌
CYTOLOGIA (ISSN:00114545)
巻号頁・発行日
vol.68, no.4, pp.389-394, 2003 (Released:2003-12-30)
参考文献数
32
被引用文献数
3 7

Cytogenetics studies on Astyanax sp B, an endemic species of the Iguaçu River (Paraná, Brazil) were carried out. The specimens showed a standard diploid number of 2n=50 chromosomes with a karyotype consisting of 6 metacentric, 24 submetacentric, 6 subtelocentric and 14 acrocentric chromosomes. In addition to the basic karyotype, all the specimens presented 1–2 supranumerary or B chromosomes in mitotic metaphases. Three morphological types of B chromosomes have been identified in population under analysis: an acrocentric-type microchromosome; a metacentric macrochromosome smaller than the first pair of the normal karyotype complement; a small submetacentric chromosome. C-band analysis shows discrete markings in the centromeric regions of various chromosomes which contrast with strongly heterochromatic blocks in the telomeric regions of the long arms of chromosome pairs 20, 21 and 22 (acrocentric). Heterochromatic block-sized heteromorphism has been among homologues of pair 20. The chromosome characterisitics of Astyanax sp B indicate similarity with other species of the genus Astyanax with regard to karyotypic macrostructure, distribution of constitutive heterochromatin (C-band pattern) and occurrence of B chromosomes. Some aspects related B chromosomes in this species are discussed.
著者
Junko Hasegawa Takumi Higaki Yuki Hamamura Daisuke Kurihara Natsumaro Kutsuna Tetsuya Higashiyama Seiichiro Hasezawa Sachihiro Matsunaga
出版者
日本メンデル協会、国際細胞学会
雑誌
CYTOLOGIA (ISSN:00114545)
巻号頁・発行日
vol.79, no.4, pp.467-474, 2014-12-25 (Released:2015-01-16)
参考文献数
31
被引用文献数
1 4

Vacuoles occupy 80–90% of a mature plant cell and mainly contribute to all types of cell expansion. Zeocin, an inducer of DNA double-strand breaks, causes cell expansion with endoreduplication. The vacuolar structure after zeocin treatment was examined in tobacco (Nicotiana tabacum) cultured cells expressing GFP fused to a vacuole membrane protein. We found that the genotoxic stress induced the cell expansion with subdivision of the vacuolar lumen by cytoplasmic strands. When a femtosecond laser was used to cut off the cytoplasmic strand, mitochondrial transport along the strand stopped. This suggested that in the elongated cells under the genotoxic stress, the transport of subcellular materials was activated for DNA repair within the damaged cell nucleus by the construction of a network of cytoplasmic strands in the vacuolar lumen.
著者
Rubens Pazza Horácio Ferreira Júlio Jr.
出版者
日本メンデル協会、国際細胞学会
雑誌
CYTOLOGIA (ISSN:00114545)
巻号頁・発行日
vol.68, no.2, pp.159-163, 2003 (Released:2003-10-20)
参考文献数
24
被引用文献数
10 24

Cytogenetical studies on Hoplias malabaricus, intensive in the last few years, have determined that it does not represent only 1 species, as it is classified, but a complex of species with 7 cytotypes , some of them living in a sympatric condition with no hybrids. In this study, for the first time, 3 cytotypes were found in sympatry and sintopy in the Upper Paraná River floodplain. Cyytotaxonomy and geographical aspects are discussed.
著者
R. J. Low K. Benirschke
出版者
Japan Mendel Society, International Society of Cytology
雑誌
CYTOLOGIA (ISSN:00114545)
巻号頁・発行日
vol.37, no.1, pp.1-11, 1972-03-25 (Released:2009-03-19)
参考文献数
19
被引用文献数
12 11

Studies of the chromosomes of a domesticated and five wild red foxes, Vulpes fulva, have produced chromosome counts of 35, 36, 37 and 38. Karyotypic analyses revealed that variations in the number of microchromosomes produced this polymorphism; moreover, it is suggested that these microchromosomes may arise from fragmentation of the macrochromosomes. Because it has not been possible to establish a consistent chromosome count for this species, it is further suggested that 2n=34 be used to describe the basic diploid count and that the number of microchromosomes present be added to this figure. Thus, a total chromosome count of 36 would, by the suggested system, be recorded as 2n=34+2m.
著者
Shuhei Ota Kenshiro Oshima Tomokazu Yamazaki Tsuyoshi Takeshita Kateřina Bišová Vilém Zachleder Masahira Hattori Shigeyuki Kawano
出版者
Japan Mendel Society, International Society of Cytology
雑誌
CYTOLOGIA (ISSN:00114545)
巻号頁・発行日
vol.84, no.4, pp.323-330, 2019-12-25 (Released:2019-12-20)
参考文献数
45

The genus Chlorella is a well-known member of the green algal class Trebouxiophyceae, which is characterized by an immotile and asexual life cycle. Here, we performed an analysis of the whole genome and transcriptome of Parachlorella kessleri NIES-2152 with emphasis on the evolution of meiosis and the flagellar proteins. The Parachlorella transcriptomic data showed that the MID-related RWP-RK genes and meiosis-specific and flagellar proteins were expressed; at the transcriptional level, the DNA repair protein RAD50 was upregulated in the stationary phase, with four-fold more reads per kilobase of transcript per million mapped reads (RPKM) compared with the early stage of culture. In contrast, radial spoke protein genes were down-regulated in the stationary phase. These results suggest that genes for meiotic and flagellar proteins are culture stage-dependent and retain their functions. We presume that the algae lost some of the genes for meiosis and the flagella during asexual evolution, but other genes still possess biological functions other than those related to the flagellum and meiosis.
著者
Yusif M. Agayev
出版者
Japan Mendel Society, International Society of Cytology
雑誌
CYTOLOGIA (ISSN:00114545)
巻号頁・発行日
vol.67, no.3, pp.245-252, 2002 (Released:2003-02-28)
参考文献数
18
被引用文献数
7 8

Karyotype analyses of saffron, Crocus sativus L., was made using improved routine and C-banding techniques on metaphase plates in root tip cells of 2 accessions from Republic of Azerbaijan and Iran. In the routine technique fixative of Lewitsky and aceto-iron-hematoxylin stain were used. It was established that metaphase chromosomes can be arranged according to their size and morphological features in 8 triplets ranging in size from 11.58±0.13 μm (triplet 1) to 4.57±0.05 μm (triplet 8). Triplets 1 and 2 include subacrocentric, triplets 3, 4 and 8 metacentric and triplets 6 and 7 submetacentric chromosomes. Three chromosomes in each triplet, as a rule, are similar although in some triplets one of them is infrequently distinguishable from the 2 others. Triplet 5 shows an extreme difference so that it always contains 2 kinds of chromosomes: chromosome 5(1) and chromosomes 5(2, 3). Chromosome 5(1) is metacentric (r=1.40) and 6.04±0.13 μm in length, but chromosomes 5(2, 3) are subacrocentric (r=3.49) and noticeably smaller (5.41±0.09 μm). Application of the C-banding technique revealed heterochromatin segments: the sharpest, sharp and week. The sharpest heterochromatin was on telomere of the short arm of chromosome type (triplet) 3 and on proximal part of the long arm of chromosome type 1; a sharp heterochromatin on telomere of the short arms of chromosome types 1, 4 and on the satellites, and a weak heterochromatin on a centromeric region all of 24 chromosomes. Chromosome type 5(1) had a heterochromatin that was a little stronger on the centromeric and, considerably weaker on telomeric regions comparing to those of chromosome type 4. Accessions of saffron from Republic of Azerbaijan and Iran were not significantly distinguishable in karyotype structure. The species C. sativus in its places of cultivation is obviously a clone of one triploid plant originated spontaneously in the nature through crossing between 2 closely related species with participation of n and 2n gametes. Results from statistic analysis and appropriate photographic evidences are provided.
著者
A. N. Bhatnagar T. F. El-Azawi
出版者
Japan Mendel Society, International Society of Cytology
雑誌
CYTOLOGIA (ISSN:00114545)
巻号頁・発行日
vol.43, no.1, pp.53-59, 1978-01-25 (Released:2009-03-19)
参考文献数
7
被引用文献数
1 4

Karyotypes are described in Hemiechinus auritus and Paraechinus aethiopicus which have 48 chromosomes in 2n. The male complement of H. auritus consists of 43 macro- and 5 micro-chromosomes while that of the female comprises 44 macro- and 4-micro-chromosomes. The X chromosome is the smallest metacentric macro-chromosome, while the Y is a small subtelocentric micro-chromosome. These associate end-to-end at meiosis I.The male karyotype of P. aethiopicus consists of 44 macro- and 4 micro-chromosomes. The autosomes are metacentrics or submetacentrics in morphology. The sex-complex is characterized by the medium-sized X chromosome and the mediumsized submetacentric Y.
著者
Pool P. A. Ingram Ruth Abbott R. J. Jennings D. L. Topham Pauline B.
出版者
財団法人 日本メンデル協会
雑誌
CYTOLOGIA (ISSN:00114545)
巻号頁・発行日
vol.46, no.1, pp.125-132, 1981
被引用文献数
7

Previous workers have reported that Rubus chromosomes are too small for karyotype analyses at mitosis, and also that meiotic studies indicate no major structural differences between Rubus genomes. However, it was found possible to identify the seven chromosomes of Glen Clova, a cultivar of R. idaeus, and of R. coreanus. The chromosomes of R. idaeus differed from those of R. coreanus, four of them appreciably, and it was possible to identify each of the 14 chromosomes of four F1hybrids between the two species. In spite of these differences, meiosis and pollen formation in the hybrids appeared normal. It is suggested that genome differentiation in the genus is greater than realised, and that further karyotype analyses may aid studies of species relationships.
著者
Ma Yan Crane Charles F. Byrne David H.
出版者
Japan Mendel Society, International Society of Cytology
雑誌
CYTOLOGIA (ISSN:00114545)
巻号頁・発行日
vol.61, no.4, pp.457-463, 1996
被引用文献数
2

Meiotic chromosome configuration frequencies at diakinesis and metaphase I were tabulated in diploid and induced tetraploid <I>Rosa laevigata</I> Michx., diploid <I>R. roxburghii</I> Tratt., and tetraploid amphidiploids of <I>R. laevigata</I> with <I>R. roxburghii</I> and <I>R. banksiae</I> Ait. Numerical analysis of configuration frequencies suggests up to two-fold length variation between short and long chromosome arms in the diploids, and incomplete independence of chromosome arms during pairing and chiasma formation in the tetraploids. Since the sampled genomes pair homeologously in intersubgeneric amphidiploids, they are relatively closely related. The meiotic data collectively suggest that <I>R. roxburghii</I> should be classified in subgenus <I>Rosa</I>.
著者
Satoru Fujimoto Sachihiro Matsunaga
出版者
Japan Mendel Society, International Society of Cytology
雑誌
CYTOLOGIA (ISSN:00114545)
巻号頁・発行日
vol.82, no.5, pp.559-562, 2017-12-25 (Released:2018-01-24)
参考文献数
28
被引用文献数
4

Labeling of specific DNA sequences in living organisms is important for understanding the mechanism of gene regulation during development, differentiation, and environmental adaptation. Here we describe a CRISPR/Cas9-based chromatin visualization system in plants. We adapted the mammalian nuclease-dead Cas9-fluorescent protein (dCas9-FP) system to a plant expression vector. Transient expression of dCas9-FP and single guide RNA (sgRNA) for telomeric sequences efficiently labeled telomere repeats in tobacco.
著者
Shigeyuki Kawano
出版者
日本メンデル協会、国際細胞学会
雑誌
CYTOLOGIA (ISSN:00114545)
巻号頁・発行日
vol.82, no.2, pp.219-219, 2017-03-25 (Released:2017-05-11)
参考文献数
1

There is an injustice in this article by Talukdar, D. and Biswas, A. K. (2007). The editorial committee decided to retract it immediately.The injustice is apparent figure manipulation; Parts of Figs. 39 and 41 were inversed and imposed in Figs. 37 and 38, respectively.
著者
G. Santhakumari J. Stephen
出版者
日本メンデル協会、国際細胞学会
雑誌
CYTOLOGIA (ISSN:00114545)
巻号頁・発行日
vol.53, no.1, pp.163-168, 1988-03-25 (Released:2009-03-19)
参考文献数
24
被引用文献数
1 2

Antimitotic effect of holothurin, a water soluble saponin isolated from the sea cucumber Holothuria vagabunda, was assessed by Allium test supplemented by autoradiography. Endoreduplication, chromosome clumping, positive and/or negative pycnosis of nuclei, nuclear polymorphism and chromatin extrusion through cytoplasmic buds were the common cytological abnormalities induced by holothurin. Inhibition of DNA and RNA synthesis was observed and this may be the molecular mechanism behind the above abnormalities. In all these features holothurin appears to be similar to Adriamycin, currently used in Cancer Chemotherapy.
著者
Toshiyuki Takahashi Mayuko Sato Kiminori Toyooka Hisayoshi Nozaki
出版者
日本メンデル協会、国際細胞学会
雑誌
CYTOLOGIA (ISSN:00114545)
巻号頁・発行日
vol.79, no.1, pp.119-123, 2014-03-25 (Released:2014-07-15)
参考文献数
18
被引用文献数
7 10

Cyanophora paradoxa is an enigmatic biflagellate that may represent the first photosynthetic eukaryote morphologically. This alga has been widely studied as a model organism of primitive phototrophs. However, surface ornamentations of the vegetative cells have not been examined using ultra-high resolution field emission (FE) scanning electron microscopy (SEM). In the present study, C. paradoxa NIES-547 vegetative cells were examined using FE-SEM and compared with the data using conventional SEM. Our FE-SEM images demonstrated that the cell surface was ornamented with angular fenestrations framed by ridges. In contrast, conventional SEM did not reveal similar surface ornamentation. Transmission electron microscopy showed the ridge was formed by the edges of overlapping or attaching outermost plate vesicles at the cell periphery.