著者

Shamimul Alam Sheikh Katsuhiko Kondo Yoshikazu Hoshi

出版者

日本メンデル協会、国際細胞学会

雑誌

CYTOLOGIA (ISSN:00114545)

巻号頁・発行日

vol.60, no.1, pp.43-47, 1995-03-25 (Released:2009-03-19)

参考文献数

11

被引用文献数

24 50

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Drosera dichrosepala was exposed to different doses of Gamma radiation. Fragmented and fused chromosomes were observed as a consequences. Diffused centromeres in every fragment chromosome was detected by centromeric banding (Cd-banding) and was supported by its typical disjunction and totally lack of lagging chromosomes or micronuclei from anaphase to telophase. C-banding revealed that the fragment chromosomes were highly heterochromatic and fragmentation might be occurred at the terminal regions of chromosomes. Fluorescent banding suggested that most of the fragment chromosomes were rich in GC base composition in the species. Alteration of karyotype due to Gamma irradiation also indicated that spontaneous fragmentation or fusion of chromosomes might be a possible factor for promoting the bimodal karyotype in this genus.

著者

Mina Ohtsu Daisuke Kurihara Yoshikatsu Sato Takuya Suzaki Masayoshi Kawaguchi Daisuke Maruyama Tetsuya Higashiyama

出版者

日本メンデル協会、国際細胞学会

雑誌

CYTOLOGIA (ISSN:00114545)

巻号頁・発行日

vol.82, no.3, pp.251-259, 2017-06-25 (Released:2017-09-06)

参考文献数

35

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Nematode infection of plant roots is a paradigm of host–parasite interactions. Although nematodes can be labeled with fluorescent dyes, migration of the worms into the deep regions of host roots makes them difficult to track. Here we report the use of two fluorescent dyes, FM4-64 and SYBR green I, to intensely label the soybean cyst nematode (SCN) Heterodera glycines for one week in host plants. Continuous monitoring of the labeled SCN juveniles was achieved with two-photon microscopy. Additionally, we developed a transient transformation system consisting of the non-model leguminous plant (fabaceous) roots, Astragalus sinicus and Agrobacterium rhizogenes to observe the cellular structures of the plant during SCN infection. By the combined use of fluorescent dyes and two-photon microscopy, clear images of infecting SCNs were obtained even in deep regions of A. sinicus roots. The fluorescent labeling described herein can also be used in detailed monitoring of the infection processes of other non-model nematodes, as well as the associated morphological changes in the host plant roots.

著者

Daniel Luis Zanella Kantek Alberto Sergio Fenocchio Marta Margarete Cestari

出版者

日本メンデル協会、国際細胞学会

雑誌

CYTOLOGIA (ISSN:00114545)

巻号頁・発行日

vol.68, no.1, pp.19-24, 2003 (Released:2003-10-10)

参考文献数

28

被引用文献数

1 4

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The characids belong to the greater freshwater fish family of the world with around 700 recognized species. Whithin this group the genus Astyanax includes several species with unclear phylogenetic relationships, showing a wide karyotypic diversity. In the highly endemic Iguaçu river basin these problems are also observed being distinguished 6 unnamed Astyanax species (A to F). In the present work were cytogenetically studied 2 populations of A. sp. C from the first plateau of the Iguaçu river (Paraná State, Brazil). Fishes of both populations are similar in chromosome number, having 2n=50, however were evidenced clear differences in C-banding and nucleolar organizing regions (NORs) patterns. With the use of the restriction enzyme Alu I were obtained the same results as C-bands. These results indicate the complexity of Astyanax and the importance of further taxonomic revisions joining morphological, cytogenetic and molecular approachings.

著者

Lana Cristina Fazoli Valquíria de Almeida Bernardo da Silva Ana Luiza de Brito Portela-Castro Horácio Ferreira Júlio Júnior

出版者

日本メンデル協会、国際細胞学会

雑誌

CYTOLOGIA (ISSN:00114545)

巻号頁・発行日

vol.68, no.4, pp.389-394, 2003 (Released:2003-12-30)

参考文献数

32

被引用文献数

3 9

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Cytogenetics studies on Astyanax sp B, an endemic species of the Iguaçu River (Paraná, Brazil) were carried out. The specimens showed a standard diploid number of 2n=50 chromosomes with a karyotype consisting of 6 metacentric, 24 submetacentric, 6 subtelocentric and 14 acrocentric chromosomes. In addition to the basic karyotype, all the specimens presented 1–2 supranumerary or B chromosomes in mitotic metaphases. Three morphological types of B chromosomes have been identified in population under analysis: an acrocentric-type microchromosome; a metacentric macrochromosome smaller than the first pair of the normal karyotype complement; a small submetacentric chromosome. C-band analysis shows discrete markings in the centromeric regions of various chromosomes which contrast with strongly heterochromatic blocks in the telomeric regions of the long arms of chromosome pairs 20, 21 and 22 (acrocentric). Heterochromatic block-sized heteromorphism has been among homologues of pair 20. The chromosome characterisitics of Astyanax sp B indicate similarity with other species of the genus Astyanax with regard to karyotypic macrostructure, distribution of constitutive heterochromatin (C-band pattern) and occurrence of B chromosomes. Some aspects related B chromosomes in this species are discussed.

著者

Junko Hasegawa Takumi Higaki Yuki Hamamura Daisuke Kurihara Natsumaro Kutsuna Tetsuya Higashiyama Seiichiro Hasezawa Sachihiro Matsunaga

出版者

日本メンデル協会、国際細胞学会

雑誌

CYTOLOGIA (ISSN:00114545)

巻号頁・発行日

vol.79, no.4, pp.467-474, 2014-12-25 (Released:2015-01-16)

参考文献数

31

被引用文献数

1 7

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Vacuoles occupy 80–90% of a mature plant cell and mainly contribute to all types of cell expansion. Zeocin, an inducer of DNA double-strand breaks, causes cell expansion with endoreduplication. The vacuolar structure after zeocin treatment was examined in tobacco (Nicotiana tabacum) cultured cells expressing GFP fused to a vacuole membrane protein. We found that the genotoxic stress induced the cell expansion with subdivision of the vacuolar lumen by cytoplasmic strands. When a femtosecond laser was used to cut off the cytoplasmic strand, mitochondrial transport along the strand stopped. This suggested that in the elongated cells under the genotoxic stress, the transport of subcellular materials was activated for DNA repair within the damaged cell nucleus by the construction of a network of cytoplasmic strands in the vacuolar lumen.

著者

Rubens Pazza Horácio Ferreira Júlio Jr.

出版者

日本メンデル協会、国際細胞学会

雑誌

CYTOLOGIA (ISSN:00114545)

巻号頁・発行日

vol.68, no.2, pp.159-163, 2003 (Released:2003-10-20)

参考文献数

24

被引用文献数

10 24

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Cytogenetical studies on Hoplias malabaricus, intensive in the last few years, have determined that it does not represent only 1 species, as it is classified, but a complex of species with 7 cytotypes , some of them living in a sympatric condition with no hybrids. In this study, for the first time, 3 cytotypes were found in sympatry and sintopy in the Upper Paraná River floodplain. Cyytotaxonomy and geographical aspects are discussed.

著者

Shigeyuki Kawano

出版者

日本メンデル協会、国際細胞学会

雑誌

CYTOLOGIA (ISSN:00114545)

巻号頁・発行日

vol.82, no.2, pp.219-219, 2017-03-25 (Released:2017-05-11)

参考文献数

1

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There is an injustice in this article by Talukdar, D. and Biswas, A. K. (2007). The editorial committee decided to retract it immediately.The injustice is apparent figure manipulation; Parts of Figs. 39 and 41 were inversed and imposed in Figs. 37 and 38, respectively.

著者

G. Santhakumari J. Stephen

出版者

日本メンデル協会、国際細胞学会

雑誌

CYTOLOGIA (ISSN:00114545)

巻号頁・発行日

vol.53, no.1, pp.163-168, 1988-03-25 (Released:2009-03-19)

参考文献数

24

被引用文献数

1 2

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Antimitotic effect of holothurin, a water soluble saponin isolated from the sea cucumber Holothuria vagabunda, was assessed by Allium test supplemented by autoradiography. Endoreduplication, chromosome clumping, positive and/or negative pycnosis of nuclei, nuclear polymorphism and chromatin extrusion through cytoplasmic buds were the common cytological abnormalities induced by holothurin. Inhibition of DNA and RNA synthesis was observed and this may be the molecular mechanism behind the above abnormalities. In all these features holothurin appears to be similar to Adriamycin, currently used in Cancer Chemotherapy.

著者

Toshiyuki Takahashi Mayuko Sato Kiminori Toyooka Hisayoshi Nozaki

出版者

日本メンデル協会、国際細胞学会

雑誌

CYTOLOGIA (ISSN:00114545)

巻号頁・発行日

vol.79, no.1, pp.119-123, 2014-03-25 (Released:2014-07-15)

参考文献数

18

被引用文献数

7 11

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Cyanophora paradoxa is an enigmatic biflagellate that may represent the first photosynthetic eukaryote morphologically. This alga has been widely studied as a model organism of primitive phototrophs. However, surface ornamentations of the vegetative cells have not been examined using ultra-high resolution field emission (FE) scanning electron microscopy (SEM). In the present study, C. paradoxa NIES-547 vegetative cells were examined using FE-SEM and compared with the data using conventional SEM. Our FE-SEM images demonstrated that the cell surface was ornamented with angular fenestrations framed by ridges. In contrast, conventional SEM did not reveal similar surface ornamentation. Transmission electron microscopy showed the ridge was formed by the edges of overlapping or attaching outermost plate vesicles at the cell periphery.