- 著者
- Jun-ichi Shiraishi Daichi Ijiri Ayumi Katafuchi Shozo Tomonaga Saki Shimamoto Hanwool Do Shinya Ishihara Akira Ohtsuka
- 出版者
- Japan Poultry Science Association
- 雑誌
- The Journal of Poultry Science (ISSN:13467395)
- 巻号頁・発行日
- vol.60, no.2, pp.2023017, 2023 (Released:2023-07-22)
- 参考文献数
- 28
- 被引用文献数
- 1
The concentration of Nτ-methylhistidine in plasma provides an index of skeletal muscle protein breakdown. This study aimed to establish a quantitative method for measuring the concentrations of Nτ-methylhistidine and its isomer Nπ-methylhistidine in chicken plasma, using liquid chromatography–tandem mass spectrometry with stable isotope dilution analysis. The acceptable linear ranges of detection were 1.56–50.00 μmol/L for Nτ-methylhistidine and 0.78–25.00 μmol/L for Nπ-methylhistidine. The proposed method detected changes in the plasma levels of Nτ-methylhistidine and Nπ-methylhistidine in response to fasting and re-feeding. These results suggest that the method developed in this study can be used for the simultaneous measurement of Nτ-methylhistidine and Nπ-methylhistidine in chicken plasma.
- 著者
- Ayumi Katafuchi Saki Shimamoto Mana Kawaguchi Shozo Tomonaga Kazuki Nakashima Shinya Ishihara Akira Ohtsuka Daichi Ijiri
- 出版者
- Japan Poultry Science Association
- 雑誌
- The Journal of Poultry Science (ISSN:13467395)
- 巻号頁・発行日
- vol.60, no.2, pp.2023032, 2023 (Released:2023-12-22)
- 参考文献数
- 34
Exogenous nutrients are essential for body and skeletal muscle growth in newly hatched chicks, and delaying post-hatch feeding negatively affects body growth, meat yield, and meat quality. The aim of this study was to investigate the effects of delayed post-hatch feeding on the metabolic profiles of broiler chickens using a combination of targeted and untargeted metabolomics. Newly hatched chicks had either immediate free access to feed (freely fed chicks) or no access to feed from 0 to 2 days of age (delayed-fed chicks); both groups were subsequently provided feed ad libitum until 13 days of age. Untargeted metabolomic analysis was performed using gas chromatography-mass spectrometry, whereas targeted metabolomic analysis of amino acids was performed using high-performance liquid chromatography with ortho-phthalaldehyde derivatization. Delayed feeding increased the plasma levels of sucrose, maltose, serotonin, lactitol, gentiobiose, xylitol, threonic acid, and asparagine, and decreased the plasma levels of creatinine, aspartic acid, and glutamic acid. In addition, the digestibility of the nitrogen-free extract (starch and sugar) and the cecal butyric acid concentration increased in chicks subjected to delayed feeding. In contrast, delayed feeding did not affect muscle protein degradation or digestibility in chicks. Taken together, our results indicate that delaying feeding until 48 h post-hatch alters multiple metabolic pathways, which are accompanied by changes in intestinal carbohydrate digestion and cecal butyric acid content in broiler chickens.