著者
Yutaka Tahara Katsuya Obara
出版者
日本家禽学会
雑誌
The Journal of Poultry Science (ISSN:13467395)
巻号頁・発行日
vol.51, no.3, pp.307-312, 2014-07-25 (Released:2014-07-25)
参考文献数
14
被引用文献数
11

The development of shell-less culture methods for bird embryos with high hatchability would be useful for the efficient generation of transgenic chickens, embryo manipulations, tissue engineering, and basic studies in regenerative medicine. To date, studies of culture methods for bird embryos include the whole embryo culture using narrow windowed eggshells, surrogate eggshells, and an artificial vessel using a gas-permeable membrane. However, there are no reports achieving high hatchability of >50% using completely artificial vessels. To establish a simple method for culturing chick embryos with high hatchability, we examined various culture conditions, including methods for calcium supplementation and oxygen aeration. In the embryo cultures where the embryos were transferred to the culture vessel after 55-56 h incubation, more than 90% of embryos survived until day 17 when a polymethylpentene film was used as a culture vessel with calcium lactate and distilled water supplementations. The aeration of pure oxygen to the surviving embryos from day 17 yielded a hatchability of 57.1% (8 out of 14). Thus, we successfully achieved a high hatchability with this method in chicken embryo culture using an artificial vessel.
著者
Awad A.Shehata Wieland Schrödl Philipp Schledorn Monika Krüger
出版者
日本家禽学会
雑誌
The Journal of Poultry Science (ISSN:13467395)
巻号頁・発行日
vol.51, no.3, pp.333-337, 2014-07-25 (Released:2014-07-25)
参考文献数
25
被引用文献数
2 6

Glyphosate (N-(phosphonomethyl) glycine) is a most popular herbicide in agricultural practices throughout the world. It is possible that glyphosate spread in the ecosystems can reach plants, animals. The present work was directed to investigate the glyphosate residue in different organs of broiler chickens using ELISA and to study the possibility of its neutralisation using humic acid, Chlorella vulgaris and Saccharomyces boulardii. Results showed that glyphosate residues could be detected in the animal feed and different organs as liver, spleen, lung, intestine, heart, muscles and kidney. Humic acid, Chlorella vulgaris and Saccharomyces boulardii showed neutralization of the antimicrobial effect of glyphosate in vitro. Also, feed supplementation of commercial broiler with humic acid (0.2%) leads to a significant decrease in the glyphosate content, i.e. by 53%, 28%, 44%, 50%, 56%, 16%, 63% and 0% in serum, liver, spleen, lung, gastro-intestinal tract, heart, muscles and kidney, respectively. There were no significant effects of humic acid on the production parameters. This enlightenment will help to overcome the negative effect of glyphosate residues on gastrointestinal microbiota and protect consumers from glyphosate residues in chicken meat.
著者
Yoichiro Shimura Nobuo Shoji Taichiro Tanikawa Tomoaki Obayashi Jun Honda Miyuki Tanaka Yukiko Sasaki Jun Fukushima Tamio Inamoto
出版者
日本家禽学会
雑誌
The Journal of Poultry Science (ISSN:13467395)
巻号頁・発行日
vol.53, no.2, pp.165-172, 2016 (Released:2016-04-25)
参考文献数
33

To determine the influence of media composition on Salmonella exclusion of Nurmi-type cultures, two and four types of cultures in the first and second trial, respectively, were prepared from the cecal contents of conventional laying hens, and Salmonella exclusion was assessed in newly hatched chicks. In the first trial, modified Viande Levure (VL) broth or nutrient broth (NB) were used to prepare Nurmi-type cultures (N-VL and N-NB), which were administered to the newly hatched chicks. Twenty-four hours later, the chicks were challenged with Salmonella enterica Typhimurium EF85-9 (ST). ST recoveries (log10 colony forming units/g of cecal contents) from the N-VL-, N-NB-, and control-treated groups 5 days after the challenge were 7.6±0.6, 0.9±1.9, and 7.7±0.4, respectively. The results suggested the influence of L-cysteine (Cys) present in the VL broth. Thus, we determined the effect of Cys in the second trial. We prepared two other cultures using VL broth without Cys (N-VL—Cys) and NB with Cys (N-NB+Cys). ST recoveries from the cecal contents of the N-VL-, N-VL—Cys-, and control-treated groups were 6.3±0.9, 2.1±2.5, and 9.2±0.8, respectively. ST was not recovered from the N-NB- and N-NB+Cys-treated groups. To identify bacteria with Salmonella exclusion activity, we isolated 41 bacterial strains from the ceca of N-NB-treated chicks without Salmonella challenge. Most isolates were identified as Enterococcus faecalis or E. mundtii based on 16S rRNA gene sequencing, and only four cultures excluded Salmonella. Therefore, VL broth containing Cys was not always required for preparing Nurmi-type cultures. The use of media prepared with Cys at the lowest possible concentration or without Cys would promote to enhance Salmonella exclusion from Nurmi-type cultures.
著者
Tahara Yutaka Obara Katsuya
出版者
日本家禽学会
雑誌
The Journal of Poultry Science (ISSN:13467395)
巻号頁・発行日
vol.51, no.3, pp.307-312, 2014
被引用文献数
11

The development of shell-less culture methods for bird embryos with high hatchability would be useful for the efficient generation of transgenic chickens, embryo manipulations, tissue engineering, and basic studies in regenerative medicine. To date, studies of culture methods for bird embryos include the whole embryo culture using narrow windowed eggshells, surrogate eggshells, and an artificial vessel using a gas-permeable membrane. However, there are no reports achieving high hatchability of >50% using completely artificial vessels. To establish a simple method for culturing chick embryos with high hatchability, we examined various culture conditions, including methods for calcium supplementation and oxygen aeration. In the embryo cultures where the embryos were transferred to the culture vessel after 55-56 h incubation, more than 90% of embryos survived until day 17 when a polymethylpentene film was used as a culture vessel with calcium lactate and distilled water supplementations. The aeration of pure oxygen to the surviving embryos from day 17 yielded a hatchability of 57.1% (8 out of 14). Thus, we successfully achieved a high hatchability with this method in chicken embryo culture using an artificial vessel.
著者
Shozo Tomonaga Mitsuhito Matsumoto Mitsuhiro Furuse
出版者
Japan Poultry Science Association
雑誌
The Journal of Poultry Science (ISSN:13467395)
巻号頁・発行日
vol.49, no.4, pp.308-312, 2012-10-25 (Released:2012-10-25)
参考文献数
22
被引用文献数
8 9

Carnosine and its methylated derivative anserine are dipeptides present in high levels in chicken muscles. They are antioxidants and putative neurotransmitters. If administration of β-alanine, one of the constituents of carnosine, could increase levels of these dipeptides in the brain and muscles, it may improve brain function and increase commercial values of the chicken meat. In the present study, we investigated whether orally administered β-alanine could increase these dipeptide levels in the brain, Musculus pectoralis superficialis, and plasma in broiler chicks. Broilers (2 days old) were given oral doses of β-alanine (0.176, 0.88, 4.4 and 22 mmol/kg) twice a day for 5 days. Carnosine levels were dose-dependently increased in the brain, Musculus pectoralis superficialis, and plasma while no influence of anserine was detected. These results suggest that supplemental β-alanine could be effective in increasing carnosine levels in the brain and muscle of broiler chicks.
著者
Sae Suzuki Misato Kobayashi Atsushi Murai Masaoki Tsudzuki Akira Ishikawa
出版者
Japan Poultry Science Association
雑誌
The Journal of Poultry Science (ISSN:13467395)
巻号頁・発行日
vol.56, no.2, pp.101-111, 2019 (Released:2019-04-25)
参考文献数
31
被引用文献数
1

Excessive fat deposition adversely affects poultry production. In this study, we investigated growth, fat deposition, and hepatic mRNA expression of 13 lipid metabolism-related genes in three unique breeds of meat-type chickens with distinct breed origins and genetic relationships. One was Nagoya (NAG), a native Japanese breed, whereas the others were White Plymouth Rock (WPR) and White Cornish (WC), which have been used worldwide as the parental breeds of common broiler chickens. NAG chickens were phenotypically characterized by slow growth, lean body fat, and high gizzard and liver weights. In contrast, both WC and WPR chickens were characterized by rapid growth but high percentage of subcutaneous fat and abdominal fat weight, resulting from high feed intake. Among the three breeds, WC had the highest percentage of pectoral muscle weight, whereas WPR was the most obese. Among lipid metabolism-related genes, the expression of PPARA, PPARG, and CD36 was mostly associated with obesity. These results provide basic information for quantitative trait locus (QTL) analysis related to growth and fat traits in an F2 population of the lean NAG breed and the obese WPR breed of meat-type chickens in future.
著者
Fujiwara Akira Mizutani Makoto Ono Tamao KAGAMI Hiroshi
出版者
日本家禽学会
雑誌
The journal of poultry science (ISSN:13467395)
巻号頁・発行日
vol.42, no.1, pp.64-69, 2005-01-25
参考文献数
13
被引用文献数
2 3

A black plumage color mutant (BPC) was described in this paper. The BPC quail was a dark colored bird. The chick down feathers on the back and head were black ending with a brown tip. The face and throat were cream-yellow and the ventral surface was a mixture of gray and cream-yellow. The beak and shanks were darkly pigmented but some toes were yellow while others had spotted areas of pigmentation.<BR> When the birds reached to maturity, both males and females had pigmentation, and the feather appearance of dorsal and ventral surface were basically similar. The dorsal feather pattern was similar to that of wild-type except that the wheat-straw shafting was absent. Some of the BPC quails showed small areas of white feather at the junction of the upper and lower beaks and in the throat under the lower beak.<BR> To clarify the mode of inheritance of the BPC characters, mating experiments were performed. The segregation data for F<SUB>1</SUB>, F<SUB>2</SUB> and backcross generations were analyzed. Genetic analysis showed that the BPC was controlled by an autosomal recessive gene. Therefore, a gene symbol &ldquo;<CITE>rb</CITE>&rdquo; was proposed.
著者
Leticia S. Murate Fernanda G. Paião Adriana M. de Almeida Angelo Berchieri Jr. Massami Shimokomaki
出版者
日本家禽学会
雑誌
The Journal of Poultry Science (ISSN:13467395)
巻号頁・発行日
vol.52, no.1, pp.52-56, 2015 (Released:2015-01-25)
参考文献数
22
被引用文献数
2 11

This study aimed to evaluate the efficacy of dietary prebiotic, probiotic, and synbiotic products for controlling infection in laying hens and broiler chickens challenged with Salmonella enterica serovar Enteritidis (SE). These products could replace the use of antibiotics, which would avoid the problem of hastening antimicrobial resistance for both types of birds. Salmonella-free 1-day-old (1-d-old) layers chicks and broilers chicks were inoculated with SE resistant to nalidixic acid and spectinomycin (SE Nalr Specr) and divided into four groups: 1) control (without feed additives); 2) probiotic (Bacillus subtilis, Lactobacillus acidophilus, L. casei, Enterococcus faecium, Bifidobacterium longum); 3) prebiotic (inulin, fructooligosaccharide, mannanoligosaccharide, and oligosaccharide); and 4) synbiotic (85% of the probiotic+15% of the prebiotic additives). The presence of SE Nalr Specr in cloacal swabs was analyzed at 7, 14, and 21 days post-infection (dpi) in laying hens and broilers. The number of SE Nalr Specr per gram of cecal contents was determined at 7, 14, and 21 dpi in laying hens and at 2, 5, 7, 14, and 21 dpi in broilers. The results showed that the prebiotic additive reduced the occurrence of SE in cloacal swabs from laying hens but not from broilers. In the groups of laying hens and broilers that received prebiotics, the isolation and counts of SE Nalr Specr were lower during the first week post-infection but not throughout the experiment. The probiotic and synbiotic additives did not influence the SE infection in laying hens and broilers; in contrast prebiotics had a protective effect during the first week post-infection.
著者
Yuichi Wajiki Yoshinori Kaneko Toshie Sugiyama Takahisa Yamada Hiroaki Iwaisaki
出版者
日本家禽学会
雑誌
The Journal of Poultry Science (ISSN:13467395)
巻号頁・発行日
pp.0150040, (Released:2015-08-25)
被引用文献数
1

The Japanese captive population of Japanese crested ibis (Nipponia nippon) was established using 5 founders derived from the Chinese captive population. Its size has increased rapidly, and the maintenance phase is about to start. Thus, this study was designed to perform genetic analyses in this population with pedigree information, considering the adoption of mean kinship strategy as the breeding strategy suited to the maintenance phase. Because the relationships among the 5 founders were unknown, different assumptions were set up ranging from 0 to 0.25 of kinship coefficients between the 5 founders. Assuming that the 5 founders were non-inbred in all the assumptions, the results showed that the gene diversity and the mean inbreeding coefficient would fluctuate largely from ∼65% to ∼82% and from ∼0.07 to ∼0.29, respectively. Moreover, the genetic importance of individuals based on mean kinship shifted largely. This study suggested that the Japanese captive population had low gene diversity and high mean inbreeding coefficient even under the assumption that the 5 founders were unrelated and non-inbred. In addition, the study also suggested that it became more effective to analyze the genetic status and to introduce mean kinship strategy into this population with more credible molecular evaluation of the relationships among founders.
著者
Witold S. Proskura Daria Cichoń Wilhelm Grzesiak Daniel Zaborski Ewa Sell-Kubiak Yeong-Hsiang Cheng Andrzej Dybus
出版者
日本家禽学会
雑誌
The Journal of Poultry Science (ISSN:13467395)
巻号頁・発行日
pp.0130237, (Released:2014-03-25)

The objective of the present study was to investigate the relationship between the g.2582481G>A, g.2583935G>A and g.2584057C>T single nucleotide polymorphisms (SNPs) within the lactate dehydrogenase A gene (LDHA) coding for lactate dehydrogenase isoform A and the racing performance of homing pigeons. As a measure of racing performance, we used the mean values of ace points won by individual birds during the whole season. The estimated heritability of the racing performance of pigeons was relatively low (h2=0.0596; SE=0.0249). The analyzis performed for all race reports together showed that the factors such as gender, weather conditions at the start and at the end of the race affect the analyzed trait. Of the 3 single nucleotide polymorphisms, only the effect of the g.2582481G>A genotype on the performance of racing pigeons was significant. Statistical analyzis indicated the difference in the value of ace points between the animals of GG and GA genotypes for the g.2582481G>A SNP. The study showed that the genotype homozygous for g.2582481A is linked to the highest mean value of ace points. Consequently, the relationship between the genotype for g.2582481G>A and the racing performance was shown.
著者
Ahmed A. Saleh Kunioki Hayashi Akira Ohtsuka
出版者
日本家禽学会
雑誌
The Journal of Poultry Science (ISSN:13467395)
巻号頁・発行日
vol.50, no.3, pp.242-250, 2013-07-25 (Released:2013-07-25)
参考文献数
45
被引用文献数
4 17

This study was conducted to examine the effects of a combined in-feed supplementation of Aspergillus awamori and Saccharomyces cerevisiae on growth and muscle protein metabolism and fatty acid profiles in broilers. Chicks (15 d old) were fed a basal diet as control, diets supplemented with 0.05% A. awamori, 0.10% S. cerevisiae, or a combination of both (7 birds/group) for 12 days. Growth of the birds was promoted by all treatments. Synergistic effects of A. awamori and S. cerevisiae were observed on body weight gain and feed conversion, breast muscle weight, and digestibility of dietary protein. Plasma 3-methylhistidine concentrations were decreased by A. awamori and S. cerevisiae, and synergistically by the combination. Gene expressions of proteolysis-related factors in muscle were reduced by all treatments. Conversely, mRNA expressions of myosin and actin were synergistically increased by the combination. Abdominal fat and plasma triglycerides were decreased by A. awamori and the combination, but not by S. cerevisiae, while muscle fat content was increased by all treatments. Interestingly, there was a decrease in saturated fatty acids and an increase in unsaturated fatty acids in muscle in all treatment groups. This change in fatty acid profile was partially related to mRNA expression of delta-6 fatty acid desaturase in the muscle. In conclusion, the combined supplementation of A. awamori and S. cerevisiae synergistically improves growth performance by promoting muscle protein metabolism. In addition, A. awamori and S. cerevisiae modify the muscle fatty acid profile.
著者
Bao-Wei Wang Guo-Qing Huang Wei-Wei Wang Wen-Hua Ge Ming-Ai Zhang
出版者
日本家禽学会
雑誌
The Journal of Poultry Science (ISSN:13467395)
巻号頁・発行日
pp.0120090, (Released:2012-11-25)

The beneficial effects of dietary selenium (Se) supplementation for poultry and the distribution of Se in tissues have been well documented, but the functionalities of the Se-enriched products are much less concerned. Alcohol induces liver injury by initiating oxidative stress and antioxidant therapy has been proposed as an effective way of reducing the injury. Because a correlation between Se and antioxidant capability has been established, the alleviation effect of the Se-enriched fatty goose liver (SFGL, containing 900 μg/kg Se) on alcohol-induced liver injury was studied. Mice were daily fed with 2.5, 5, and 10 g/kg·bw SFGL and common fatty goose liver (CFGL, containing 200 μg/kg Se) one hour after ethanol administration and the animals were scarified and the blood and liver were collected for analysis on day 21. Results indicated that SFGL was more effective in reducing the hepatosomatic index and the degree of steatosis than CFGL. SFGL in all the three doses normalized the lipid metabolism and the activities of ALT and AST, indicating that SFGL attenuated the alcohol-induced liver injury. SFGL decreased the serum and hepatic ADH activities and the MDA content, implying that the liver injury attenuation effect of SGFL was related to oxidative stress reduction. The measurement of antioxidant variables, including GSH-Px, GSH, and SOD, revealed that the levels of these molecules were significantly increased after SFGL ingestion, confirming that SFGL alleviated alcohol-induced liver injury by enhancing the antioxidant capability. It was concluded that Se-enriched poultry tissues are potential remediators against alcohol-induced liver injury.