- 著者
- Xiaoyin SUN Takao YASUI Takeshi YANAGIDA Noritada KAJI Sakon RAHONG Masaki KANAI Kazuki NAGASHIMA Tomoji KAWAI Yoshinobu BABA
- 出版者
- (社)日本分析化学会
- 雑誌
- Analytical Sciences (ISSN:09106340)
- 巻号頁・発行日
- vol.33, no.6, pp.735-738, 2017-06-10 (Released:2017-06-10)
- 参考文献数
- 10
- 被引用文献数
- 1
Here, we developed a device integrated with a nanochannel and nanostructures to slow DNA translocation velocity. We found that translocation velocity of a single DNA molecule inside a nanochannel was decreased by pre-elongating it using some nanostructures, such as a shallow channel or nanopillars. This decrease of the translocation velocity was associated with the DNA mobility change, which is an intrinsic parameter of DNA molecules and unaffected by an electric field.
1 0 0 0 OA Self-assembled Nanowire Arrays as Three-dimensional Nanopores for Filtration of DNA Molecules
- 著者
- Sakon RAHONG Takao YASUI Takeshi YANAGIDA Kazuki NAGASHIMA Masaki KANAI Gang MENG Yong HE Fuwei ZHUGE Noritada KAJI Tomoji KAWAI Yoshinobu BABA
- 出版者
- (社)日本分析化学会
- 雑誌
- Analytical Sciences (ISSN:09106340)
- 巻号頁・発行日
- vol.31, no.3, pp.153-157, 2015-03-10 (Released:2015-03-10)
- 参考文献数
- 23
- 被引用文献数
- 3 12
Molecular filtration and purification play important roles for biomolecule analysis. However, it is still necessary to improve efficiency and reduce the filtration time. Here, we show self-assembled nanowire arrays as three-dimensional (3D) nanopores embedded in a microfluidic channel for ultrafast DNA filtration. The 3D nanopore structure was formed by a vapor-liquid-solid (VLS) nanowire growth technique, which allowed us to control pore size of the filtration material by varying the number of growth cycles. λ DNA molecules (48.5 kbp) were filtrated from a mixture of T4 DNA (166 kbp) at the entrance of the 3D nanopore structure within 1 s under an applied electric field. Moreover, we observed single DNA molecule migration of T4 and λ DNA molecules to clarify the filtration mechanism. The 3D nanopore structure has simplicity of fabrication, flexibility of pore size control and reusability for biomolecule filtration. Consequently it is an excellent material for biomolecular filtration.