著者

Yuki IWAHANA Atsushi OHBUCHI Yuya KOIKE Masaru KITANO Toshihiro NAKAMURA

出版者

(社)日本分析化学会

雑誌

Analytical Sciences (ISSN:09106340)

巻号頁・発行日

vol.29, no.1, pp.61-66, 2013-01-10 (Released:2013-01-10)

参考文献数

26

被引用文献数

5 19

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Radioactive nuclides in the incinerator ashes of municipal solid wastes were determined by γ-ray spectrometry before and after the accident at the Fukushima nuclear power plant (March 11, 2011). Incinerator ash samples were collected in northern Kyushu, Japan, which is located approximately 1200 km west-southwest (WSW) of the Fukushima nuclear power plant, from April 2006 to March 2007 and from March 2011 to October 2011. 40K, 137Cs, 208Tl, 212Pb, 214Pb, 212Bi, 214Bi, and 228Ac were identified in the ashes before the accident (∼February 2011) and 134Cs was identified along with these eight nuclides in the ashes after the accident (March 2011∼). A sequential extraction procedure based on a modified Tessier method with added water extraction was used for 1st fly ash sampled in August 2011 because the highest activity concentrations of 134Cs and 137Cs were observed for this sample. The speciation of radioactive nuclides in the fly ash was achieved by γ-ray spectrometry and powder X-ray diffractometry for the extraction residues. Little variation was observed in the distribution of the chemical forms of 134Cs and 137Cs in 1st fly ash of municipal solid waste; one half of 134Cs existed as water soluble salts and the other half as carbonate compounds, whereas 75% of 137Cs existed as water soluble salts with the remainder as carbonates(10%) and sulfides (15%). These results show that 88% of the total radioactive Cs existed in water soluble and ion extractive forms and might be at risk for elution and diffusion with rain and wind.

著者

Hiroshi IWANAGA Hirohiko TSUZUKI Yoichiro KAMIYAMA Hiroshi UEDA

出版者

(社)日本分析化学会

雑誌

Analytical Sciences (ISSN:09106340)

巻号頁・発行日

vol.25, no.9, pp.1101-1106, 2009-09-10 (Released:2009-09-10)

参考文献数

13

被引用文献数

1 2 3

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With a specific and strong molecular-recognition capability cultivated in humoral acquired immunity, an antibody has been extensively utilized in various applications, such as diagnostics and therapy. However, so far most of its uses have been limited to be in the liquid phase. In view of its potential uses, such as a gas-phase biosensor or a high-performance air filter, we have tried to verify a previously undescribed binding reaction between protein antigens and corresponding antibodies immobilized on a solid surface by using fluorescence resonance energy transfer between the two. Our data showed that the antibody on a solid surface specifically reacted with a protein antigen supplied from the gas phase under the normal ambient condition. Also discovered was that the reaction occurred even faster than that in the liquid phase under several assay conditions.

著者

Tomoko OHTA Yasunori MAHARA Takumi KUBOTA Toshifumi IGARASHI

出版者

(社)日本分析化学会

雑誌

Analytical Sciences (ISSN:09106340)

巻号頁・発行日

vol.29, no.10, pp.941-947, 2013-10-10 (Released:2013-10-10)

参考文献数

30

被引用文献数

1 4

---

We measured 134Cs and 137Cs in the surface soil of the Kanto loam in the eastern Tokyo metropolitan area and the Nishiyama loam in Nagasaki, Japan. The observed 137Cs deposition in the Kanto loam from the Fukushima nuclear power plant (NPP) accident ranged from 4.0 to 77 kBq m−2, which corresponds to 0.3 – 5 times of that in the Nishiyama loam. The 137Cs retardation factor in the Kanto loam obtained seven months after the Fukusima NPP accident and in the Nishiyama loam after 36 and 38 years from the detonation of the Pu atomic bomb (A-bomb) ranged from 180 to 260 and 2000 to 10000, respectively. This difference in the retardation factors is attributed to an aging effect that corresponds to seven months and 36 to 38 years after the deposition of 137Cs occurred on the soil minerals.

著者

Takeshi OHNO Atsuko SHINOHARA Ichitaro KOHGE Momoko CHIBA Takafumi HIRATA

出版者

(社)日本分析化学会

雑誌

Analytical Sciences (ISSN:09106340)

巻号頁・発行日

vol.20, no.4, pp.617-621, 2004 (Released:2004-10-08)

参考文献数

18

被引用文献数

33 54

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Precise 56Fe/54Fe and 57Fe/54Fe isotopic ratios on human red blood cell (RBC) samples have been measured using multiple collector-ICP-mass spectrometry (MC-ICPMS). The mass spectrometric interferences on Fe isotopes (e.g., 56ArO+ and 57ArOH+) were successfully minimized by a dry plasma condition achieved by a desolvating nebulizer sample-introduction technique. In order to eliminate possible variations in the measured isotopic ratios due to non-mass spectrometric interferences, Fe was separated from remaining organic compounds and major co-existing elements using an ion chromatographic technique. The resulting precisions of the 56Fe/54Fe and 57Fe/54Fe ratio measurements were 0.12‰ and 0.20‰, respectively, which were high enough to detect the isotopic variation of Fe in nature. For an interlaboratory comparison, all of the Fe isotopic ratio data were normalized by the ratios for the IRMM-014 international isotopic standard. A series of 12 RBC samples were collected from one person through monthly-based sampling over a period of one year. These were analyzed to test possible seasonal changes in the 56Fe/54Fe and 57Fe/54Fe ratios. Moreover, in order to test possible variations in the 56Fe/54Fe and 57Fe/54Fe ratios among different people, RBC samples were collected from five volunteers (four males and one female). The 56Fe/54Fe and 57Fe/54Fe ratios for a series of 12 RBC samples collected over a one-year period show 3.06‰ and 4.51‰ lower than the values of IRMM-014, and no significant seasonal change could be found in the ratios. The lack in seasonal changes in the Fe isotopic ratios could be explained by a small contribution of the daily net-intake of Fe (1 - 2 mg/day) onto the total amount of Fe in the human body (2 - 4 g). The 56Fe/54Fe and 57Fe/54Fe ratios for RBC samples collected from four male samples did not vary measurably, whereas the Fe isotopic ratios for a female RBC were 0.3‰/amu heavier than the mean value of four male samples. This difference in Fe isotopes among the individuals can be the result of a difference in uptake efficiency of the Fe through a dietary process from the digestive tract. The data obtained here demonstrate that the isotopic ratios of trace metals can provide new information about metabolic efficiencies of the metallic elements.

著者

Hideaki ISHIDA Makoto HANDA Masahiro MIKURIYA

出版者

(社)日本分析化学会

雑誌

X-ray Structure Analysis Online (ISSN:18833578)

巻号頁・発行日

vol.30, pp.9-10, 2014 (Released:2014-03-21)

参考文献数

6

被引用文献数

6

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The deuterated title compound, aqua adduct of ruthenium 3,4,5-tri(ethoxy-d5)benzoate, diaquatetrakis(μ-3,4,5-tri(ethoxy-d5)benzoato-O,O′)diruthenium(1+) tetrafluoroborate-tetrahydrofuran (1/1), [Ru2{3,4,5-(C2D5O)3CH2CO2}4(H2O)2]BF4·C4H8O, was synthesized. The crystal structure was determined by the single-crystal X-ray diffraction method at 90 K. It crystallizes in the monoclinic space group C2/c with a = 27.243(4)Å, b = 10.4850(15)Å, c = 23.115(3)Å, β = 100.789(3)°, V = 6486.1(16)Å3, Dx = 1.506 g/cm3, and Z = 4. The R1 [I > 2σ(I)] and wR2 (all data) values are 0.0460 and 0.1037, respectively, for all 6597 independent reflections. The complex cation has a lantern-like dinuclear ruthenium core with axial water molecules [Ru-Ru 2.2629(8)Å].

著者

Shenghui MEI Leting ZHU Xingang LI Jiaqing WANG Xueyun JIANG Haiyan CHEN Jiping HUO Li YANG Song LIN Zhigang ZHAO

出版者

(社)日本分析化学会

雑誌

Analytical Sciences (ISSN:09106340)

巻号頁・発行日

vol.33, no.6, pp.665-670, 2017-06-10 (Released:2017-06-10)

参考文献数

25

被引用文献数

13

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Methotrexate (MTX) plasma concentration is routinely monitored to guide the dosage regimen of rescue drugs. This study aims to develop and validate an ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method for plasma MTX analysis, and to establish its agreement with the fluorescence polarization immunoassay (FPIA) in patients with high-dose MTX therapy. Separation was achieved by gradient elution with methanol and water (0.05% formic acid) at 40°C with a run time of 3 min. The intra- and inter-day inaccuracy and imprecision of the UPLC-MS/MS method were –4.25 to 3.1 and less than 7.63%, respectively. The IS-normalized recovery and matrix effect were 87.05 to 92.81 and 124.43 to 134.57%. The correlation coefficients between UPLC-MS/MS and FPIA were greater than 0.98. The UPLC-MS/MS method was in agreement with the FPIA at high levels of MTX (1.0 – 100 μmol/L), but not at low levels (0.01 – 1.0 μmol/L). Further studies are warranted to confirm these results.

著者

Xiaoyin SUN Takao YASUI Takeshi YANAGIDA Noritada KAJI Sakon RAHONG Masaki KANAI Kazuki NAGASHIMA Tomoji KAWAI Yoshinobu BABA

出版者

(社)日本分析化学会

雑誌

Analytical Sciences (ISSN:09106340)

巻号頁・発行日

vol.33, no.6, pp.735-738, 2017-06-10 (Released:2017-06-10)

参考文献数

10

被引用文献数

1

---

Here, we developed a device integrated with a nanochannel and nanostructures to slow DNA translocation velocity. We found that translocation velocity of a single DNA molecule inside a nanochannel was decreased by pre-elongating it using some nanostructures, such as a shallow channel or nanopillars. This decrease of the translocation velocity was associated with the DNA mobility change, which is an intrinsic parameter of DNA molecules and unaffected by an electric field.

著者

Toshimasa TOYO’OKA

出版者

(社)日本分析化学会

雑誌

Analytical Sciences (ISSN:09106340)

巻号頁・発行日

vol.33, no.5, pp.555-564, 2017-05-10 (Released:2017-05-10)

参考文献数

91

被引用文献数

40

---

Liquid chromatography coupled with mass spectrometry (LC-MS) is one of the most prominent analytical techniques due to its inherent selectivity and sensitivity. LC-MS is currently the first choice for high-throughput bioanalysis due to the advancements in MS instruments and the analytical software. Based on this situation, we are developing various types of derivatization reagents, including chiral reagents for MS and/or MS/MS detection. These developed reagents are adopted for the detection of biomarker candidates related to diseases. The biomarker candidates include not only achiral molecules, but also chiral ones. Although determining the already-identified chiral molecules is relative easy, it is very difficult to identify and/or determine unknown enantiomer(s) in real samples. To solve this difficulty, we proposed a new strategy to identify unknown enantiomeric biomarkers related to diseases. This review paper deals with the development of derivatization reagents for amines and carboxylic acids in LC-MS analysis and their application to bioanalysis.

著者

Aiying SONG

出版者

(社)日本分析化学会

雑誌

Analytical Sciences (ISSN:09106340)

巻号頁・発行日

vol.32, no.6, pp.645-652, 2016-06-10 (Released:2016-06-10)

参考文献数

48

被引用文献数

12

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Pesticides and antidepressants are frequently misused in drug-facilitated crime because of their toxicological effect and easy-availability. Therefore, it is essential for the development of a simple and reliable method for the determination of these organic toxicants in biological fluids. Here, we report on an applicable method by the combination of optimized liquid–liquid extraction (LLE) procedure and high-performance liquid chromatography–tandem mass spectrometry (HPLC-MS/MS) to identify and quantify dimethoate, omethoate, dichlorvos, carbofuran, fenpropathrin, diazepam, estazolam, alprazolam, triazolamm, chlorpromazine, phenergan, barbitone and phenobarbital in human blood. The method demonstrated a linear calibration curve in range of 20 – 500 μg/L (r > 0.994). The accuracy evaluated by recovery spiked at three different concentrations (50, 100 and 200 μg/L) was in the range of 58.8 – 83.1% with a relative standard deviations (RSD) of 3.7 – 7.4%. The limits of quantification ranged over 6.7 – 33.3 μg/L. This method was proved to be simple and reliable, and was thus successfully applied to forensic toxicology.

著者

Kohei OTOMO Terumasa HIBI Takashi MURATA Hirotaka WATANABE Ryosuke KAWAKAMI Hiroshi NAKAYAMA Mitsuyasu HASEBE Tomomi NEMOTO

出版者

(社)日本分析化学会

雑誌

Analytical Sciences (ISSN:09106340)

巻号頁・発行日

vol.31, no.4, pp.307-313, 2015-04-10 (Released:2015-04-10)

参考文献数

28

被引用文献数

2 33

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The temporal resolution of a two-photon excitation laser scanning microscopy (TPLSM) system is limited by the excitation laser beam’s scanning speed. To improve the temporal resolution, the TPLSM system is equipped with a spinning-disk confocal scanning unit. However, the insufficient energy of a conventional Ti:sapphire laser source restricts the field of view (FOV) for TPLSM images to a narrow region. Therefore, we introduced a high-peak-power Yb-based laser in order to enlarge the FOV. This system provided three-dimensional imaging of a sufficiently deep and wide region of fixed mouse brain slices, clear four-dimensional imaging of actin dynamics in live mammalian cells and microtubule dynamics during mitosis and cytokinesis in live plant cells.

著者

Shigehisa UCHIYAMA Kazushi OHTA Yohei INABA Naoki KUNUGITA

出版者

(社)日本分析化学会

雑誌

Analytical Sciences (ISSN:09106340)

巻号頁・発行日

vol.29, no.12, pp.1219-1222, 2013-12-10 (Released:2013-12-10)

参考文献数

11

被引用文献数

33 157 4

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Carbonyl compounds in E-cigarette smoke mist were measured using coupled silica cartridges impregnated with hydroquinone and 2,4-dinitrophenylhydrazine, followed by high-performance liquid chromatography. A total of 363 E-cigarettes (13 brands) were examined. Four of the 13 E-cigarette brands did not generate any carbonyl compounds, while the other nine E-cigarette brands generated various carbonyl compounds. However, the carbonyl concentrations of the E-cigarette products did not show typical distributions, and the mean values were largely different from the median values. It was elucidated that E-cigarettes incidentally generate high concentrations of carbonyl compounds.

著者

Koji FURUKAWA Makoto HASHIMOTO Satoshi KANECO

出版者

(社)日本分析化学会

雑誌

Analytical Sciences (ISSN:09106340)

巻号頁・発行日

vol.33, no.10, pp.1189-1191, 2017-10-10 (Released:2017-10-10)

参考文献数

14

被引用文献数

4

---

A rapid determination of aniline in environmental water was examined based on liquid chromatography/tandem mass spectrometry (LC/MS/MS). Environmental water samples were diluted 20-fold with Mill-Q water and measured by LC/MS/MS after adding a surrogate substance (aniline-d5). In the results of the present study, the calibration curve of aniline showed good linearity in the range of 0.05 – 2.0 μg/L. Since the RSD (repeatability) by measuring repeatedly an aniline standard solution (0.05 μg/L, n = 7) was 3.2%, the repeatability of this work was very excellent. In addition, the recovery rate of aniline in environmental water was in the range of 99.0 – 102% with RSD 3.4 – 7.7%, and very good recovery test results were obtained. From these results, this analytical method was confirmed to be effective for aniline measurements of environmental water samples. Also, it is possible to conduct rapid analyses of aniline in environmental water without any solid-phase extraction process, compared to the solid-phase extraction-GC/MS method.

著者

Andrei BITA George Dan MOGOSANU Ludovic Everard BEJENARU Carmen Nicoleta OANCEA Cornelia BEJENARU Octavian CROITORU Gabriela RAU Johny NEAMTU Iulia Daria SCOREI Ion Romulus SCOREI John HUNTER Brad EVERS Boris NEMZER Florin ANGHELINA Otilia-Constantina ROGOVEANU

出版者

(社)日本分析化学会

雑誌

Analytical Sciences (ISSN:09106340)

巻号頁・発行日

vol.33, no.6, pp.743-746, 2017-06-10 (Released:2017-06-10)

参考文献数

20

被引用文献数

4

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The paper describes a new, simple, selective and precise high-performance thin-layer chromatographic (HPTLC) method for the simultaneous identification and quantitative determination of boric acid (BA) and calcium fructoborate (CFB) in bulk and tablet/capsule dosage forms of dietary supplements. HPTLC silica gel G 60 F254 precoated glass plates were used as the stationary phase. The mobile phase consisted of 2-propanol–water 8:2 (v/v). The two boron-based compounds were adequately separated with the Rf values of 0.83 ± 0.01 (BA) and 0.59 ± 0.01 (CFB).

著者

Nobuyuki TAKAYAMA Lee Wah LIM Toyohide TAKEUCHI

出版者

(社)日本分析化学会

雑誌

Analytical Sciences (ISSN:09106340)

巻号頁・発行日

vol.33, no.5, pp.619-625, 2017-05-10 (Released:2017-05-10)

参考文献数

24

被引用文献数

12

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The retention behavior of inorganic anions was studied in hydrophilic interaction chromatography (HILIC). In this study, five kinds of HILIC stationary phases (amino, imidazole, amide, pyridine and zwitterionic) were investigated. It was found that only amino and imidazole columns exhibited the separation of inorganic anions under HILIC conditions. The retention mechanism was further investigated under both columns. A reversed elution order of inorganic anions was observed under the HILIC condition compared with those observed under the ion-exchange chromatography mode (IEC). The effect of salt species and their concentration in the eluent were investigated under constant acetonitrile (ACN) content. Sodium chloride and sodium perchlorate were chosen as the salt, and the salt (sodium perchlorate) concentration was varied from 10 to 40 mM to confirm the effect of the electrostatic interaction. The slope values of the plots of the log retention factor (k) versus the log eluent concentration were calculated to be between –0.43 and –0.45 for the amino column, while those obtained on the imidazole column were between –0.68 and –0.73. Various concentrations of ACN were also examined with 20 mM sodium perchlorate, and the typical HILIC retention behavior was observed on both amino and imidazole columns. Due to the obtained results, it is considered that the separation of inorganic anions under the HILIC condition was achieved by both electrostatic interaction and partition.

著者

Shubo DONG Zhengyu YAN Hanyue YANG Zhen LONG

出版者

(社)日本分析化学会

雑誌

Analytical Sciences (ISSN:09106340)

巻号頁・発行日

vol.33, no.3, pp.293-298, 2017-03-10 (Released:2017-03-10)

参考文献数

40

被引用文献数

4

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A sensitive non-derivatization method for the determination of the highly polar compound 3-aminopiperidine was developed using a mixed-mode column combined with a charged aerosol detector (CAD). Chromatographic conditions, including the type of detector, separation mode, and mobile phase composition, were optimized to achieve high sensitivity towards and sufficient retention of 3-aminopiperidine. Compared to the precolumn derivatization UV method, the current method showed higher recovery and greater simplicify. High sensitivity (LOQ <2.73 μg mL−1) and good precision (RSD of peak area <2%) were also observed in the current method. Furthermore, the parameters such as buffer solution and column bleed that affected the sensitivity of the CAD were investigated. Finally, the current method was applied for the determination of 3-aminopiperidine in linagliptin samples. This is a new non-derivative for the determination of 3-aminopiperidine, and constitutes a novel application of the CAD for the quantitative analysis of highly polar basic compounds.

著者

Tao YANG Fang ZHU Tianxiao ZHOU Jianzhong CAO Yibo XIE Meiyi ZHANG Yan WANG Dong-Sheng CAO Qinlu LIN Lin ZHANG

出版者

(社)日本分析化学会

雑誌

Analytical Sciences (ISSN:09106340)

巻号頁・発行日

vol.33, no.2, pp.191-196, 2017-02-10 (Released:2017-02-10)

参考文献数

43

被引用文献数

7

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This study aimed to develop a label-free, sensitive, selective, and environment-friendly fluorescent peptide probe His-His-Trp-His (HHWH) for determining the concentration of copper ion (Cu2+) in aqueous solutions. The results demonstrated that the designed HHWH has a high selectivity and sensitivity for monitoring the concentration of free Cu2+ via quenching of the probe fluorescence upon a binding of Cu2+. The fluorescence intensity of the HHWH had a linear relationship with the concentration of Cu2+ between 10 nM and 10 μM, and the detection limit was 8 nM. Furthermore, HHWH could be regenerated with sulfide ions at least five times. The concentrations of Cu2+ in three different real water samples were detected using this probe, and the results were consistent with the one detected using an atomic absorption spectrometer. Thus, HHWH can be used as an accurate and feasible fluorescent peptide probe for detecting Cu2+ in aqueous solutions.

著者

Takahiro IWAI Koichi CHIBA Tomohiro NARUKAWA

出版者

(社)日本分析化学会

雑誌

Analytical Sciences (ISSN:09106340)

巻号頁・発行日

vol.32, no.9, pp.957-962, 2016-09-10 (Released:2016-09-10)

参考文献数

22

被引用文献数

11

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The concentrations of arsenic (As) and cadmium (Cd) in the tobacco leaves, ash and smoke of 10 kinds of cigarettes collected from different countries worldwide were determined by ICP-MS after microwave-assisted digestion. Total As and Cd concentrations in the tobacco leaves ranged from 0.20 to 0.63 and 1.8 to 9.9 mg kg−1, respectively. By the speciation analysis of As in tobacco leaves and ash by HPLC-ICP-MS following acid extraction, arsenite [As(III)] and arsenate [As(V)] were determined and trace amounts of monomethylarsonic acid (MMAA), dimethylarsinic acid (DMAA), trimethylarsine oxide (TMAO), tetramethylarsonium (TeMA) and some unidentified As species were also found. Arsenic speciation for smoke absorbed in an aqueous solution was carried out. The sum of the As species in tobacco leaves, ash and smoke was in good agreement with the result of total As determination in each sample, and the recoveries of speciation were 100 ± 10%. The distributions and the behaviors of As species were clarified.

著者

Shuang-Qing ZHANG

出版者

(社)日本分析化学会

雑誌

Analytical Sciences (ISSN:09106340)

巻号頁・発行日

vol.32, no.6, pp.631-637, 2016-06-10 (Released:2016-06-10)

参考文献数

17

被引用文献数

7

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Icaritin (ICT), a major component in herb Epimedium brevicornum Maxim., shows beneficial effects for the treatment of osteoporosis and various cancers, and is predominantly metabolized to glucuronidated icaritin (GICT). Although clinical trials of ICT have exhibited good safety and tolerance, the dynamic bioditributions of ICT and GICT have not been reported. In the present study, the chemical structure of GICT was firstly reported, and a reliable ultra-high performance liquid chromatography–tandem mass spectrometry method (UHPLC-MS/MS) was firstly established for the simultaneous quantifications of ICT and GICT in rat tissues. The dynamic distribution of ICT and GICT in rat tissues and their pharmacokinetic parameters have been reported for the first time. ICT, GICT and the internal standard coumestrol were separated on a C18 column with a gradient mobile phase of acetonitrile and water containing ammonium formate and formic acid at a flow rate of 0.3 mL min−1. The analytes were quantified by a triple quadrupole tandem mass spectrometer in the negative ionization mode. The lower limit of quantification values for ICT and GICT were 0.2 and 2 ng mL−1, respectively. Good selectivity, linearity, accuracy, precision and recovery were achieved, and no significant matrix effect was observed. The UHPLC-MS/MS was firstly applied to a dynamic biodistribution study of ICT and GICT in rats, following an intraperitoneal administration of ICT at a dose of 10 mg kg−1.

著者

Xiaozhen GUO Hao WU Shiwen GUO Yating SHI Juanli DU Panpan ZHU Liming DU

出版者

(社)日本分析化学会

雑誌

Analytical Sciences (ISSN:09106340)

巻号頁・発行日

vol.32, no.7, pp.763-768, 2016-07-10 (Released:2016-07-10)

参考文献数

32

被引用文献数

16

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Polymeric ionic liquid-coated magnetic nanoparticles have been successfully prepared as adsorbents for the magnetic solid-phase extraction of four drugs, namely alfuzosin, doxazosin, terazosin and prazosin, from pharmaceutical preparations, urine samples and plasma samples. The four drugs were detected by fluorescence spectrophotometer. Several extraction parameters, including the pH of the solution; the type, ratio and volume of the desorbing reagent; the amount of adsorbent; the time of the extraction and desorption processes; and the addition of NaCl, were investigated and optimized. Linear responses were determined for the four drugs in the concentration range of 0.5 – 45 ng mL−1. The limit of detection values for alfuzosin, doxazosin, terazosin and prazosin, which were defined as three times the standard deviation of a blank sample, were determined to be 0.035, 0.034, 0.027 and 0.028 ng mL−1 (n = 11), respectively. Furthermore, this new method gave preconcentration factors of 114.5, 111.3, 111.1 and 108.5 for these four drugs.

著者

Weiwen HU Jian ZHANG Jinming KONG

出版者

(社)日本分析化学会

雑誌

Analytical Sciences (ISSN:09106340)

巻号頁・発行日

vol.32, no.5, pp.523-527, 2016-05-10 (Released:2016-05-10)

参考文献数

43

被引用文献数

4

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Herein, we applied hypericin (Hyp) for the detection of DNA on streptavidin-functionalized magnetic beads (SA-MB) through non-covalent π-π stacking interaction between 1-pyrenebutanoic acid (PBA) and Hyp. Briefly, the target DNA (tDNA) was hybridized to morpholino oligonucleotide (MO) that was immobilized on the surface of SA-MB. PBA was incorporated to the backbone of tDNA by means of phosphate-zirconium-carboxylate coordination reaction. Then, fluorescent Hyp was linked to PBA via π-π stacking interaction. Under the optimal conditions, this biosensor displayed a good linear relationship between the fluorescence intensity and the tDNA concentrations in the range from 1 to 100 nM with a low detection limit of 2.9 nM. The mismatch type in a specific DNA sequence can also be efficiently discriminated. Compared with some other reports, this method is more convenient, and has great potential for practical applications.