- 著者
- Makoto Oba Mika Shibuya Yuto Yamaberi Hidetomo Yokoo Satoshi Uchida Atsushi Ueda Masakazu Tanaka
- 出版者
- The Pharmaceutical Society of Japan
- 雑誌
- Chemical and Pharmaceutical Bulletin (ISSN:00092363)
- 巻号頁・発行日
- vol.71, no.3, pp.250-256, 2023-03-01 (Released:2023-03-01)
- 参考文献数
- 23
- 被引用文献数
- 3
Amphipathic peptides composed of cationic amino acids and hydrophobic amino acids have cell-penetrating ability and are often used as a delivery tool for membrane-impermeable compounds. Small interfering RNA (siRNAs) are one of the delivery targets for such cell-penetrating peptides (CPPs). Cationic CPPs can associate with anionic siRNAs by electrostatic interactions resulting in the formation of nano-sized complexes, which can deliver siRNAs intracellularly. CPPs containing unnatural amino acids offer promising tools to siRNA delivery. However, the detailed structure–activity relationship in siRNA delivery has been rarely studied. In the current study, we designed peptides containing dipropylglycine (Dpg) and explored the cellular uptake and cytotoxicity of peptide/siRNA complexes. The amphipathic structure of the peptides played a key role in complexation with siRNAs and intracellular siRNA delivery. In the amphipathic peptides, cellular uptake of siRNA increased with increasing peptide length, but cytotoxicity was reduced. A peptide containing four Dpg exhibited an effective gene-silencing effect with small amounts of peptides without cytotoxicity in medium containing serum. These findings will be helpful for the design of novel CPPs for siRNA delivery.
- 著者
- Sota OKABE Satoshi UCHIDA Yukiko MATSUI Masaki YAMAGATA Masashi ISHIKAWA
- 出版者
- 公益社団法人 電気化学会
- 雑誌
- Electrochemistry (ISSN:13443542)
- 巻号頁・発行日
- vol.85, no.10, pp.671-674, 2017-10-05 (Released:2017-10-05)
- 参考文献数
- 13
- 被引用文献数
- 6
We prepared activated carbon (AC) and sulfur (S) composite cathodes. The present AC has developed micropores and a large pore volume. The composite could successfully include a large amount of S and exhibited stable cycle performance in a glyme-based electrolyte. An oxidation treatment of the AC with a concentrated nitric acid solution at 120°C was found to improve S utilization in charge-discharge cycling. A cathode with the oxidized AC including S showed stable cycle performance with a high capacity in not only the glyme-based electrolyte but also a carbonate-based electrolyte.
- 著者
- Shinya USUKI Satoshi UCHIDA Yukiko MATSUI Masaki YAMAGATA Hidenori HINAGO Masashi ISHIKAWA
- 出版者
- 公益社団法人 電気化学会
- 雑誌
- Electrochemistry (ISSN:13443542)
- 巻号頁・発行日
- vol.85, no.10, pp.650-655, 2017-10-05 (Released:2017-10-05)
- 参考文献数
- 22
- 被引用文献数
- 10
Micropore-rich activated carbon with high surface area and pore volume was prepared by alkali activation of azulmic carbon (AZC) precursor as nitrogen-doped carbon; AZC was a carbonized product from azulmic acid. We successfully loaded a large amount of sulfur (S) into micropores of the activated AZC. Our two kinds of activated AZC (BET surface area: 1,747 and 2,319 m2 g−1) can include S up to 55 and 62 wt%, respectively. The former activated AZC including S can be charged and discharged with lithium (Li) ion transfer stably and reversibly in glyme-based and carbonate-based electrolytes. The latter activated AZC can be charged and discharged in a glyme-based electrolyte. These different characteristics are due to a difference in fine pore structure between them. Our microporous activated AZC with high S loading is promising material as positive S electrode for rechargeable Li-S batteries.
- 著者
- Ludmila Cojocaru Satoshi Uchida Yoshitaka Sanehira Jotaro Nakazaki Takaya Kubo Hiroshi Segawa
- 出版者
- (社)日本化学会
- 雑誌
- Chemistry Letters (ISSN:03667022)
- 巻号頁・発行日
- pp.150068, (Released:2015-02-17)
- 被引用文献数
- 104
A compact TiO2 interlayer between FTO and perovskite in planar heterojunction perovskite solar cells is essential for effective charge extraction and minimized recombination. The electrical property of the interface of the perovskite layer with the compact TiO2 layer is thus important for achieving high power conversion efficiency in the perovskite solar cells. The motivation for the present study was to improve cell performance by improving the property of the TiO2/perovskite interface. Interestingly, a remarkable enhancement in cell efficiency was achieved by treatment of the compact TiO2 layer with TiCl4 and UV(O3).