著者

Yusuke Okazaki Tuyen Thi Nguyen Arisa Nishihara Hisashi Endo Hiroyuki Ogata Shin-ichi Nakano Hideyuki Tamaki

出版者

Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles

雑誌

Microbes and Environments (ISSN:13426311)

巻号頁・発行日

vol.38, no.1, pp.ME22102, 2023 (Released:2023-03-15)

参考文献数

20

被引用文献数

1

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We herein propose a fast and easy DNA and RNA co-extraction method for environmental microbial samples. It combines bead beating and phenol-chloroform phase separation followed by the separation and purification of DNA and RNA using the Qiagen AllPrep DNA/RNA mini kit. With a handling time of ~3 h, our method simultaneously extracted high-quality DNA (peak size >10–15‍ ‍kb) and RNA (RNA integrity number >6) from lake bacterioplankton filtered samples. The method is also applicable to low-biomass samples (expected DNA or RNA yield <50‍ ‍ng) and eukaryotic microbial samples, providing an easy option for more versatile eco-genomic applications.

著者

Kazuyuki Amano Shin-ichi Nakano Koichi Yamazaki

出版者

一般社団法人 情報処理学会

雑誌

Journal of Information Processing (ISSN:18826652)

巻号頁・発行日

vol.23, no.3, pp.252-257, 2015 (Released:2015-05-15)

参考文献数

5

被引用文献数

1

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The anti-slide packing is a packing of a number of three dimensional pieces of same size into a larger box such that none of them can slide in any direction. In this paper, we consider the problem of how to find a sparsest anti-slide packing. We give an IP formulation of this problem, and obtain the solutions for some small cases by using an IP solver. In addition, we give the upper and lower bounds on the ratio of the volume occupied by the pieces when the size of a box approaches infinity. For the case of piece size 2 × 2 × 1, we show that a sparsest anti-slide packing occupies at least 28.8% and at most 66.7% of the total volume.

著者

Takahiro MIYOSHI Shin-ichi NAKANO Katsuyuki NAKAMURA Keitaro YAMANOUCHI Masugi NISHIHARA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.12-0195, (Released:2012-05-31)

被引用文献数

1 5

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Adult urodele amphibians such as newts are capable of regenerating lost structures including their limbs. In these species, dedifferentiation of myofiber is essential for the regenerative process. Upon terminal differentiation, nuclei of myofiber (myonuclei) are withdrawn from cell cycle, but prior to dedifferentiation, myonuclei reenter the cell cycle. In contrast with urodele amphibians, it is generally accepted that mammalian myofibers are not able to dedifferentiate in response to muscle injury. A recent study has suggested that electroporation can induce dedifferentiation response of skeletal muscle in newt limbs. In the present study, we examined whether myonuclei of skeletal muscle of mammals are capable of reentering the cell cycle by means of electroporation. Electroporation was applied to tibialis anterior muscle of the rat with or without plasmid DNA. Histological analyses revealed that, while electroporation induces degenerative/regenerative responses in skeletal muscle irrespective of the presence of plasmid DNA, the expression of proliferating cell nuclear antigen (PCNA) in myonuclei was observed only in the presence of plasmid DNA. The present results indicate that myonuclei of skeletal muscle are capable of reentering the cell cycle and suggest that in vivo electroporation can induce dedifferentiation of mammalian skeletal muscle.