著者

Kazuo Ogawa Hirotaka Mizuochi Tomofumi Yamaguchi Sho Shirakashi Nanami Asai Yasuo Agawa

出版者

The Japanese Society of Fish Pathology

雑誌

魚病研究 (ISSN:0388788X)

巻号頁・発行日

vol.55, no.4, pp.117-124, 2021-01-15 (Released:2021-01-21)

参考文献数

23

被引用文献数

5

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The grow-out stage of leopard coral grouper Plectropomus leopardus reared in land-based tanks in Okinawa Prefecture, Japan, were often heavily infected with capsalid monogeneans, up to a few millimeters in size, on the fins and skin. ​Morphological investigation revealed that the parasite belongs to Benedenia (Monogenea: Capsalidae). ​Benedenia akajin n. sp. is proposed here and described morphologically, based on the unique structures in the vaginal duct with a thick-walled distal one-third, the penis with a teat-shaped tip and the testes smaller than the germarium. ​A positive relationship between the lengths of three haptoral sclerites (anterior sclerites and anterior and posterior hamuli) and body length indicates that these taxonomically important structures keep growing as the parasite ages. ​Molecular analyses showed that the present species is most closely related to B. epinepheli and B. sargocentron, with the highest 28S rDNA identity of 98.1%, but no identical sequences were found in the database. ​Both morphological and molecular evidences suggest Benedenia akajin n. sp. as an undescribed species, and this new skin fluke deserves further investigations for the stable aquaculture of P. leopardus.

著者

Satoshi Kawato Keigo Kobayashi Sho Shirakashi Soetsu Yanagi Yutaka Fukuda Hirofumi Yamashita Reiko Nozaki Ikuo Hirono Hidehiro Kondo

出版者

The Japanese Society of Fish Pathology

雑誌

魚病研究 (ISSN:0388788X)

巻号頁・発行日

vol.54, no.2, pp.27-33, 2019-06-15 (Released:2019-08-03)

参考文献数

26

被引用文献数

4

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Infection with the skin fluke Benedenia seriolae is a serious threat of yellowtail aquaculture. ​Development of genomic resources and molecular markers for B. seriolae is an essential infrastructure to develop control strategies against B. seriolae infections. ​As a seminal study to establish the genomic resources of the Japanese B. seriolae populations, we sequenced the complete mitochondrial genome (mitogenome) sequences of 12 B. seriolae specimens collected from four prefectures in Japan. ​The Japanese B. seriolae mitogenomes were mutually 99% identical, while the identity was 85% when compared to that of an Australian specimen. ​Furthermore, the gene arrangement in the Japanese B. seriolae mitogenome was slightly different from that in the Australian specimen. ​The substantial mitogenomic divergence between the Australian and Japanese specimens indicates that B. seriolae is composed of molecularly divergent populations. ​Variable sites were dispersed in the mitogenome of the Japanese B. seriolae. ​Phylogenetic relationships among the Japanese B. seriolae specimens did not reflect the host species or geographic distributions within Japan.