著者

Satoshi TAKAGI Yumiko KAGAWA Kiwamu HANAZONO Shoko MURAKAMI Tatsuya DEGUCHI Yusuke IZUMI Kenji HOSOYA Sangho KIM Masahiro OKUMURA

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.18-0148, (Released:2018-07-13)

被引用文献数

1

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A 10-year-old cat presented for evaluation with a 1-month history of salivation and oral bleeding. A right mandibular mass was palpated and computed tomography examination revealed entire bone proliferation. Mandibular bone biopsy was performed, and histopathological diagnosis was vascular hamartoma. The cat suddenly died on day 140.

著者

Satoshi TAKAGI Yumiko KAGAWA Kiwamu HANAZONO Shoko MURAKAMI Tatsuya DEGUCHI Yusuke IZUMI Kenji HOSOYA Sangho KIM Masahiro OKUMURA

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.80, no.9, pp.1456-1458, 2018 (Released:2018-09-26)

参考文献数

18

被引用文献数

1

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A 10-year-old cat presented for evaluation with a 1-month history of salivation and oral bleeding. A right mandibular mass was palpated and computed tomography examination revealed entire bone proliferation. Mandibular bone biopsy was performed, and histopathological diagnosis was vascular hamartoma. The cat suddenly died on day 140.

著者

Yukie Narasaki Yumiko Obayashi Sayami Ito Shoko Murakami Jun-Young Song Kei Nakayama Shin-Ichi Kitamura

出版者

The Japanese Society of Fish Pathology

雑誌

魚病研究 (ISSN:0388788X)

巻号頁・発行日

vol.53, no.1, pp.1-9, 2018 (Released:2018-04-03)

参考文献数

36

被引用文献数

3

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Miamiensis avidus is the causative agent of scuticociliatosis in various marine fish species. The virulence factors of the parasite have not been identified, so far. In this study, we examined M. avidus extracellular proteinases (ECPs) as potential virulence factors, using culture supernatants as an ECPs source. We investigated the substrate specificity of ECPs using artificial peptides, and the cytotoxicity of the ECPs was examined using CHSE-214 cells. To elucidate the role of ECPs in ciliate growth, M. avidus was cultured on CHSE-214 cells in the presence of proteinase inhibitors. We detected proteinase activities from the supernatant of M. avidus. Viable CHSE-214 cells decreased significantly in number, when incubated in a medium supplemented with the culture supernatant of M. avidus. The growth of ciliates on CHSE-214 cells was delayed in the presence of PMSF (serine proteinase inhibitor) and E-64 (cysteine proteinase inhibitor). These results suggested that the culture supernatant contained ECPs showing cytotoxicity, and the proteinases facilitated nutrient uptake by the ciliates. Thus, ECPs may be responsible for virulence factors of M. avidus.