著者

Keisuke Miyake Nobuyoshi Azuma Chugo Rinoie Shusaku Maeda Akima Harada Liu Li Itsunari Minami Shigeru Miyagawa Yoshiki Sawa

出版者

The Japanese Circulation Society

雑誌

Circulation Journal (ISSN:13469843)

巻号頁・発行日

pp.CJ-22-0257, (Released:2022-09-27)

参考文献数

46

被引用文献数

2

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Background: Although regenerative cell therapy is expected to be an alternative treatment for peripheral artery disease (PAD), many regenerative cell therapies have failed to show sufficient efficacy in clinical trials. Most preclinical studies have used acute ischemia models, despite PAD being a chronic disease. In addition, aging and atherosclerosis decrease the quality of a patient’s stem cells. Therefore, using a non-acute ischemic preclinical model and stem cells with high regenerative potency are important for the development of effective regenerative therapy. In this study, we assessed the tissue regenerative potential of umbilical cord-derived mesenchymal stromal cells (UCMSCs), which could potentially be an ideal cell source, in a rat model of established ischemia.Methods and Results: The regenerative capacity of UCMSCs was analyzed in terms of angiogenesis and muscle regeneration. In vitro analysis showed that UCMSCs secrete high amounts of cytokines associated with angiogenesis and muscle regeneration. In vivo experiments in a rat non-acute ischemia model showed significant improvement in blood perfusion after intravenous injection of UCMSCs compared with injection of culture medium or saline. Histological analysis revealed UCMSCs injection enhanced angiogenesis, with an increased number of von Willebrand factor-positive microcapillaries, and improved muscle regeneration.Conclusions: These results suggest that intravenous administration of UCMSCs may be useful for treating patients with PAD.

著者

Hisashi Motomura Ayaka Deguchi Takaharu Hatano Heishiro Fujikawa Shusaku Maeda Ayami Nobuyama

出版者

Japan Society for Surgical Wound Care

雑誌

International Journal of Surgical Wound Care (ISSN:24352128)

巻号頁・発行日

vol.1, no.1, pp.3-8, 2020 (Released:2020-06-16)

参考文献数

25

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Foot ulcers due to diabetes or/and arteriosclerosis obliterans frequently result in an intractable condition which is refractory to treatment. In order to overcome such a condition, establishing a treatment modality based on the tissue, infection/inflammation, moisture balance, and edge of wound (TIME) concept to clinically perform wound bed preparation (WBP), as advocated by Schulz, is important. Therefore, the following steps are essential: 1) control and treat the disease-causing chronic wounds and 2) evaluate the wounds as per the TIME concept and perform local treatment at the proper timing. The core of this treatment concept is considered to be debridement. Debridement is a procedure carried out to protect the surrounding tissue by cleaning the wound of infectious and necrotic tissues, and this is crucial for chronic foot diseases. Our current strategy consists of two steps: initial medical treatment and subsequent aggressive treatment of the lesion. Our method of performing maintenance debridement, using trafermin spray and aggressive conservative therapy using NPWT, makes TIME-based wound bed preparation possible. Proper debridement at the proper time contributes to the success of this treatment. In this paper, we report the essential factors for debridement and our methods of debridement.

著者

Shusaku Maeda Shuhei Nakanishi Masayasu Yoneda Tomokazu Awaya Kiminori Yamane Tsutomu Hirano Nobuoki Kohno

出版者

Japan Atherosclerosis Society

雑誌

Journal of Atherosclerosis and Thrombosis (ISSN:13403478)

巻号頁・発行日

pp.1112200459, (Released:2011-12-21)

参考文献数

34

被引用文献数

37 55

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Aim: Small dense low-density lipoprotein (sdLDL) has been suggested to be more atherogenic than large buoyant LDL. High-density lipoprotein (HDL) consists of two major subfractions (HDL2, HDL3), and just as controversy remains regarding which of the two is the more powerful negative risk factor for atherosclerosis, associations between sdLDL and these HDL subfractions are unclear.Methods: We measured sdLDL cholesterol (sdLDL-C), HDL2 cholesterol (HDL2-C) and HDL3 cholesterol (HDL3-C) by a newly developed method in 481 Japanese-Americans who were not using lipid-lowering medication, and examined the associations of these cholesterol concentrations with variables related to atherosclerosis.Results: In multivariate analysis, sdLDL-C was positively correlated with the body mass index (BMI), fasting glucose and insulin, 2-h glucose, HOMA-IR, high sensitivity C-reactive protein (hsCRP), and carotid artery intima-media thickness (IMT) after adjustment for age and sex. In particular, sdLDL-C was positively correlated with IMT, even after adjustment for sex, age, smoking status, hypertension, diabetes mellitus and hsCRP. HDL2-C was more closely inversely correlated than total HDL-C with BMI, fasting glucose and insulin, 2-h glucose, HOMA-IR, and hsCRP, whereas HDL3-C was not correlated with these factors. Additionally, HDL2-C was more closely correlated than total HDL-C or HDL3-C with sdLDL-C, LDL-C, triglycerides (TG), and apolipoprotein B (apoB).Conclusions: SdLDL-C was closely associated with insulin resistance and glucose tolerance, lending credence to its potential as a useful risk marker in assessing carotid artery IMT and the present degree of atherosclerosis in Japanese-Americans. The findings also suggest that subjects with higher HDL2-C levels were better protected from atherosclerosis.