著者

Taeko Shibaya Chika Kuroda Shinobu Nakayama Chiharu Minami Akiko Obara Takayoshi Fujii Sachiko Isobe

出版者

Japanese Society of Breeding

雑誌

Breeding Science (ISSN:13447610)

巻号頁・発行日

pp.20120, (Released:2021-03-30)

---

In carrot (Daucus carota L), the taproot colors orange, yellow and white are determined mostly by the Y, Y2, and Or loci. One of the most severe issues in carrot seed production is contamination by wild white carrot. To evaluate the contamination ratio, easily detectable DNA markers for white carrot are desired. To develop PCR-based DNA markers for the Y2 locus, we have re-sequenced two orange-colored carrot cultivars at our company (Fujii Seed, Japan), as well as six white- and one light-orange-colored carrots that contaminated our seed products. Within the candidate region previously reported for the Y2 locus, only one DNA marker, Y2_7, clearly distinguished white carrots from orange ones in the re-sequenced samples. The Y2_7 marker was further examined in 12 of the most popular hybrid orange cultivars in Japan, as well as ‘Nantes’ and ‘Chantenay Red Cored 2’. The Y2_7 marker showed that all of the orange cultivars examined had the orange allele except for ‘Beta-441’. False white was detected in the orange-colored ‘Beta-441’. The Y2_7 marker detected white root carrot contamination in an old open-pollinated Japanese cultivar, ‘Nakamura Senkou Futo’. This marker would be a useful tool in a carrot seed quality control for some cultivars.

著者

Kazufumi Nagata Yasunori Nonoue Kazuki Matsubara Ritsuko Mizobuchi Nozomi Ono Taeko Shibaya Kaworu Ebana Eri Ogiso-Tanaka Takanari Tanabata Kazuhiko Sugimoto Fumio Taguchi-Shiobara Jun-ichi Yonemaru Yusaku Uga Atsunori Fukuda Tadamasa Ueda Shin-ichi Yamamoto Utako Yamanouchi Toshiyuki Takai Takashi Ikka Katsuhiko Kondo Tomoki Hoshino Eiji Yamamoto Shunsuke Adachi Jian Sun Noriyuki Kuya Yuka Kitomi Ken Iijima Hideki Nagasaki Ayahiko Shomura Tatsumi Mizubayashi Noriyuki Kitazawa Kiyosumi Hori Tsuyu Ando Toshio Yamamoto Shuichi Fukuoka Masahiro Yano

出版者

Japanese Society of Breeding

雑誌

Breeding Science (ISSN:13447610)

巻号頁・発行日

vol.73, no.3, pp.332-342, 2023 (Released:2023-07-27)

参考文献数

64

被引用文献数

1

---

Many agronomic traits that are important in rice breeding are controlled by multiple genes. The extensive time and effort devoted so far to identifying and selecting such genes are still not enough to target multiple agronomic traits in practical breeding in Japan because of a lack of suitable plant materials in which to efficiently detect and validate beneficial alleles from diverse genetic resources. To facilitate the comprehensive analysis of genetic variation in agronomic traits among Asian cultivated rice, we developed 12 sets of chromosome segment substitution lines (CSSLs) with the japonica background, 11 of them in the same genetic background, using donors representing the genetic diversity of Asian cultivated rice. Using these materials, we overviewed the chromosomal locations of 1079 putative QTLs for seven agronomic traits and their allelic distribution in Asian cultivated rice through multiple linear regression analysis. The CSSLs will allow the effects of putative QTLs in the highly homogeneous japonica background to be validated.