文献一覧: Takahisa MURATA (著者)

4 0 0 0 OA Urinary lipid production profile in canine patients with splenic mass

著者: Taiki KIDA Arisa YAMAZAKI Tatsuro NAKAMURA Koji KOBAYASHI Sho YOSHIMOTO Shingo MAEDA Takayuki NAKAGAWA Ryohei NISHIMURA Takahisa MURATA
出版者: JAPANESE SOCIETY OF VETERINARY SCIENCE
雑誌: Journal of Veterinary Medical Science (ISSN:09167250)
巻号頁・発行日: pp.22-0355, (Released:2022-09-06)

Polyunsaturated fatty acids (PUFAs), including arachidonic acid (AA), docosahexaenoic acid (DHA), and eicosapentaenoic acid (EPA), are metabolized to various lipid mediators. The profile of these lipid metabolites excreted into the urine reflects inflammatory state of the body and disease conditions. In this study, we quantified 156 types of lipids in urine samples of dogs with splenic mass, using liquid chromatography-tandem mass spectrometry. We found that metabolites of prostaglandin (PG) E2, F2α, and D2, 8-iso-PGF3α, lyso-platelet activating factor, and 14,15-leukotrien C4 significantly increased in urine samples of dogs with splenic mass compared to that of healthy dogs. These observations may reflect general inflammatory responses and will help better understanding of the canine splenic mass.

2022-09-14 00:52:08
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2 0 0 0 OA The urinary lipid profile in cats with idiopathic cystitis

著者: Shinya TAKENOUCHI Yui KOBAYASHI Tatsuya SHINOZAKI Koji KOBAYASHI Tatsuro NAKAMURA Tomohiro YONEZAWA Takahisa MURATA
出版者: JAPANESE SOCIETY OF VETERINARY SCIENCE
雑誌: Journal of Veterinary Medical Science (ISSN:09167250)
巻号頁・発行日: pp.22-0049, (Released:2022-04-05)

Although feline idiopathic cystitis (FIC) distresses of many cats, its pathogenesis is unknown and the diagnosis is challenging. Polyunsaturated fatty acids (PUFAs) are metabolized into various lipid mediators. Lipid mediators such as prostaglandins (PGs) modulate inflammation and many of them are excreted into the urine. Thus, the investigation of the urinary lipid profile may reveal pathogenesis and help diagnosis of FIC. We collected urine samples from five FIC cats by spontaneous urination and analyzed 158 types of lipid mediators in urines using liquid chromatography-mass spectrometry. The urinary levels of PUFAs were higher in FIC compared to those of the healthy group. The excretions of a major inflammatory mediator, PGD2, were less in FIC. Other well-known inflammatory mediators such as PGE2, PGI2, and their metabolites did not show a difference. In contrast, the levels of PGF2α and its 2 metabolites and PGF3α were higher in FIC. These results may provide new insights into the future management of cat FIC.

2022-04-07 23:21:07
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1 0 0 0 OA The urinary lipid profile in cats with idiopathic cystitis

著者: Shinya TAKENOUCHI Yui KOBAYASHI Tatsuya SHINOZAKI Koji KOBAYASHI Tatsuro NAKAMURA Tomohiro YONEZAWA Takahisa MURATA
出版者: JAPANESE SOCIETY OF VETERINARY SCIENCE
雑誌: Journal of Veterinary Medical Science (ISSN:09167250)
巻号頁・発行日: vol.84, no.5, pp.689-693, 2022 (Released:2022-05-17)
参考文献数: 37

2023-07-30 10:17:47
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1 0 0 0 OA Comprehensive profiling of lipid metabolites in urine of canine patients with liver mass

著者: Taiki KIDA Arisa YAMAZAKI Tatsuro NAKAMURA Koji KOBAYASHI Sho YOSHIMOTO Shingo MAEDA Takayuki NAKAGAWA Ryohei NISHIMURA Takahisa MURATA
出版者: JAPANESE SOCIETY OF VETERINARY SCIENCE
雑誌: Journal of Veterinary Medical Science (ISSN:09167250)
巻号頁・発行日: pp.22-0191, (Released:2022-06-08)
被引用文献数: 2

Fatty acids are an essential component of mammalian bodies. They go through different metabolic pathways depending on physiological states and inflammatory stimuli. In this study, we conducted a liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based comprehensive analysis of lipid metabolites in urine of canine patients with liver mass. There were significant differences in quantity of some lipid metabolites that may be closely associated with the disease and/or general inflammatory responses, including increased metabolites of PGE2 and/or PGF2α. We demonstrated that our approach of profiling lipid metabolites in the urine is useful in gaining insights into the disease. These findings may also have an application as a screening test or a diagnosis tool for canine liver mass.

2022-06-09 15:29:54
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1 0 0 0 OA The profile of urinary lipid metabolites in healthy dogs

著者: Taiki KIDA Arisa YAMAZAKI Koji KOBAYASHI Tatsuro NAKAMURA Takayuki NAKAGAWA Ryohei NISHIMURA Takahisa MURATA
出版者: JAPANESE SOCIETY OF VETERINARY SCIENCE
雑誌: Journal of Veterinary Medical Science (ISSN:09167250)
巻号頁・発行日: pp.22-0020, (Released:2022-03-23)
被引用文献数: 2

Polyunsaturated fatty acids, including arachidonic acid (AA), docosahexaenoic acid (DHA), and eicosapentaenoic acid (EPA), are converted to hundreds of lipid mediators by cyclooxygenases (COX), lipoxygenases (LOX), and cytochrome P450 (CYP), or through non-enzymatic processes, and they reflect inflammatory states of the body. We comprehensively analyzed lipid metabolites in dog urine using a liquid chromatograph-mass spectrometry (LC-MS/MS) to describe their metabolic characteristics. We detected 31 AA-derived metabolites, four EPA-derived metabolites, and a DHA-derived metabolite in all urine samples. Among AA-derived metabolites, 15, 5, 3, and 8 were generated by COX, LOX, CYP, and non-enzymatic oxidation respectively. This study will be the first step to use profiles of urinary lipid metabolites for better understanding and diagnosis of canine diseases.

2022-03-25 02:44:40
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1 0 0 0 OA The profile of urinary lipid metabolites in cats with bacterial cystitis

著者: Yui KOBAYASHI Tatsuro NAKAMURA Tomohiro YONEZAWA Koji KOBAYASHI Takahisa MURATA
出版者: JAPANESE SOCIETY OF VETERINARY SCIENCE
雑誌: Journal of Veterinary Medical Science (ISSN:09167250)
巻号頁・発行日: pp.21-0433, (Released:2021-11-05)
被引用文献数: 1

Bacterial cystitis is one of the feline lower urinary tract diseases (FLUTDs). Polyunsaturated fatty acids, such as arachidonic acid (ARA), docosahexaenoic acid (DHA), and eicosapentaenoic acid (EPA), are oxidized into various lipid mediators that modulate inflammation. Since the profile of lipid metabolites excreted in urine is useful for assessing inflammatory body conditions, we analyzed 126 types of urinary lipid metabolites in cats with bacterial cystitis. Using LC-MS/MS, we found that the levels of 11 metabolites were higher in the urine of cystitis cats than in the urine of healthy cats. In detail, the urinary levels of ARA, EPA, and DHA and eight of their metabolites were increased in cystitis cats. Focusing on the lipid oxidation pathway, the urinary levels of four cyclooxygenase-, three lipoxygenase-, and one cytochrome p450-dependent oxidated metabolites were increased in bacterial cystitis. These urinary lipid profiles can provide some insight into the pathology and future diagnosis of bacterial cystitis.

2021-11-05 23:34:50
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1 0 0 0 OA The profile of urinary lipid metabolites in cats

著者: Yui KOBAYASHI Tatsuro NAKAMURA Koji KOBAYASHI Takahisa MURATA
出版者: JAPANESE SOCIETY OF VETERINARY SCIENCE
雑誌: Journal of Veterinary Medical Science (ISSN:09167250)
巻号頁・発行日: pp.20-0238, (Released:2020-06-05)
被引用文献数: 5

Polyunsaturated fatty acids including arachidonic acid (AA), docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), are converted to lipid mediators by oxidation. Unlike other mammals, cats cannot synthesize AA. Since their lipid metabolic features remain unknown, we qualitatively analyzed 118 types of urinary lipid metabolites in healthy neutered cats. Using LC-MS, we found 26 lipid metabolites in urines of all individuals. In detail, 20 AA-, 5 EPA- and 1 DHA-derived lipid mediators were detected. Focusing on oxidative pathway, 17 cyclooxygenase-metabolites and 5 metabolites produced by non-enzymatic pathway were detected. Of interest, few lipoxygenase- or cytochrome P450-metabolites were excreted. Thus, AA-derived cyclooxygenase-metabolites mainly composed the urinary lipid metabolites in cats.

2020-06-06 06:04:35
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