1 0 0 0 IR フットサル選手の誕生月に関する一考察 : 男女別優秀選手の比較から
- 著者
- 河合 一武 高藤 順 太田 真司 黒澤 尚 山本 大 北上 優 Kazutake Kawai Jun Takafuji Shinji Ota Takashi Kurosawa Dai Yamamoto Yu Kitakami
- 出版者
- 武蔵丘短期大学
- 雑誌
- 武蔵丘短期大学紀要 (ISSN:13413120)
- 巻号頁・発行日
- vol.19, pp.155-159, 2011
- 著者
- Kazuya EDAMURA Takashi KUROSAWA Rei NAKANO Kenji TESHIMA Kazushi ASANO Shigeo TANAKA
- 出版者
- JAPANESE SOCIETY OF VETERINARY SCIENCE
- 雑誌
- Journal of Veterinary Medical Science (ISSN:09167250)
- 巻号頁・発行日
- vol.74, no.6, pp.817-820, 2012 (Released:2012-07-04)
- 参考文献数
- 15
- 被引用文献数
- 2 2
We investigated the influence of autologous serum (AS)-supplemented medium on the proliferation and differentiation into neurons of canine bone marrow stromal cells (BMSCs). Canine BMSCs were cultured using α-MEM only, α-MEM with 10% fetal bovine serum (FBS), and 5, 10 and 20% AS-supplemented α-MEM. Growth of canine BMSCs was observed in all AS groups. The proliferation capacity of canine BMSCs in the AS groups was similar to that in the FBS group. No significant differences between the FBS and AS groups were observed in the percentage of the cells that changed to the neuron-like morphology and neuron-specific enolase-positive ratio after neuronal differentiation. Canine BMSCs cultured using AS-supplemented medium were able to proliferate and showed neuronal differentiation potency.
- 著者
- Kazuya EDAMURA Takashi KUROSAWA Rei NAKANO Kenji TESHIMA Kazushi ASANO Shigeo TANAKA
- 出版者
- JAPANESE SOCIETY OF VETERINARY SCIENCE
- 雑誌
- Journal of Veterinary Medical Science (ISSN:09167250)
- 巻号頁・発行日
- pp.1201210761, (Released:2012-01-27)
- 被引用文献数
- 2 2
We investigated the influence of autologous serum (AS)-supplemented medium on the proliferation and differentiation into neurons of canine bone marrow stromal cells (BMSCs). Canine BMSCs were cultured using α-MEM only, α-MEM with 10% fetal bovine serum (FBS), and 5%, 10%, and 20% AS-supplemented α-MEM. Growth of canine BMSCs was observed in all AS groups. The proliferation capacity of canine BMSCs in the AS groups was similar to that in the FBS group. No significant differences between the FBS and AS groups were observed in the percentage of the cells that changed to the neuron-like morphology and neuron-specific enolase-positive ratio after neuronal differentiation. Canine BMSCs cultured using AS-supplemented medium were able to proliferate and showed neuronal differentiation potency.