- 著者
- Tomoko ARAYA-KOJIMA Tomoko YAESHIMA Norio ISHIBASHI Seiichi SHIMAMURA Hirotoshi HAYASAWA
- 出版者
- Japan Bifidus Foundation
- 雑誌
- Bifidobacteria and Microflora (ISSN:02869306)
- 巻号頁・発行日
- vol.14, no.2, pp.59-66, 1995 (Released:2010-06-28)
- 参考文献数
- 11
- 被引用文献数
- 30 36
The inhibitory effects of human-derived Bifidobacterium longum BB536 on harmful intestinal bacteria were examined by co-cultivation of BB536 with each one of the following eight bacterial strains: Escherichia coli, Klebsiella pneumoniae, Clostridium clostridiiforme, C. perfringens, Bacteroides distasonis, B. fragilis, B. thetaiotaomicron and B. vulgatus. In comparison with the results of mono-cultivation, BB536 inhibited both the growth of these putrefactive bacteria and their production of ammonia, and decreased the pH of the culture medium by producing lactic and acetic acids. Enzy-matic assays showed that in BB536 the sorts of the enzymes involved in ammonia production (urease and amino acid deaminases) were rather few and their activities were weaker than observed in the harmful bacteria, whereas the activities of enzymes involved in ammonia assimilation (glutamine synthetase, glutamate synthase and glutamate dehydrogenase) were much higher in BB536 than in the putrefactive bacteria.
- 著者
- Tomoko YAESHIMA Sachiko TAKAHASHI Nobuko MATSUMOTO Norioi ISHIBASHI Hirotoshi HAYASAWA Hisakazu IINO
- 出版者
- JAPAN BIFIDUS FOUNDATION
- 雑誌
- Bioscience and Microflora (ISSN:13421441)
- 巻号頁・発行日
- vol.16, no.2, pp.73-77, 1997 (Released:2010-06-28)
- 参考文献数
- 26
- 被引用文献数
- 32 42
Yogurt containing Bifidobacterium longum BB536 (designated as Bifidus yogurt) was administered to adult volunteers and its effects on the intestinal environment with reference to fecal microflora, ammonia levels, fecal characteristics (color, consistency) and defecation frequency were examined. Bifidus yogurt was manufactured by fermenting milk with B. longum BB536, Streptococcus thermophilus STH-450 and Lactobacillus delbrueckii subsp. bulgaricus LBU-108. Standard yogurt manufactured using only S. thermophilus STH-450 and L. delbrueckii subsp. bulgaricus LBU-108 was used as the control diet. Eleven women volunteers were assigned as subjects to test the effects of Bifidus yogurt on the intestinal environment. Thirty-nine women volunteers were assigned as subjects to test the effects on fecal characteristics and defecation frequency. The volunteers were each administered 100 g of standard yogurt per day for two weeks. After a two-week interval period, each subject was administered 100 g of Bifidus yogurt per day for the subsequent test period. The period of administration of Bifidus yogurt was 2 weeks for testing effects on the intestinal environment and 3 weeks for testing effects on fecal characteristics and defecation frequency. The administration of Bifidus yogurt was effective to increase the number and relative percentage of fecal bifidobacteria significantly. The fecal ammonia concentration tended to decrease and fecal organic acid content tended to increase. The defecation frequency was significantly increased by Bifidus yogurt. The color of the feces changed to yellow and the consistency changed to soft. The administration of Bifidus yogurt was effective to improve the intestinal environment, fecal characteristics and defecation frequency.