著者
Tsubasa Shoji Kazuki Saito
出版者
Japanese Society for Plant Biotechnology
雑誌
Plant Biotechnology (ISSN:13424580)
巻号頁・発行日
pp.22.1113a, (Released:2023-01-23)
参考文献数
28
被引用文献数
1

A group of anti-nutritional specialized metabolites called steroidal glycoalkaloids (SGAs) are produced in Solanum species such as tomato, potato, and eggplant. The transcription factor JASMONATE-RESPONSIVE ETHYLENE RESPONSE FACTOR 4 (JRE4) regulates many SGA biosynthesis genes in tomato and potato. Here we report that the expression of a cluster of genes encoding nitrate transporter 1/peptide transporter family (NPF) members is downregulated in the jre4-1 loss-of-function tomato mutant, which has a low-SGA phenotype compared to the wild type. NPFs are a large family of plant membrane transporters that transport a wide range of substrates, including specialized metabolites. We found that the JRE4-regulated NPF genes are induced by the defense-related phytohormone jasmonate. Conversely, jasmonate-mediated induction of gene expression was attenuated by ethylene treatment of the leaves. The co-regulation of the NPF genes with SGA biosynthesis genes by JRE4 suggests that NPF transporters are involved in the SGA pathway.
著者
Tsubasa Shoji Kazuki Saito
出版者
Japanese Society for Plant Biotechnology
雑誌
Plant Biotechnology (ISSN:13424580)
巻号頁・発行日
vol.39, no.4, pp.421-425, 2022-12-25 (Released:2022-12-25)
参考文献数
15
被引用文献数
1

RING membrane-anchor (RMA) E3 ubiquitin ligases are involved in endoplasmic reticulum (ER)-associated protein degradation, which mediates the regulated destruction of ER-resident enzymes in various organisms. We determined that the transcription factor JASMONATE-RESPONSIVE ETHYLENE RESPONSE FACTOR 4 (JRE4) co-regulates the expression of the RMA-type ligase gene SlRMA1, but not its homolog SlRMA2, with steroidal glycoalkaloid biosynthesis genes in tomato, perhaps to prevent the overaccumulation of these metabolites.