- 著者
-
SHIOZAKI Shozo
SHIMIZU Sakayu
YAMADA Hideaki
- 出版者
- Japan Society for Bioscience, Biotechnology, and Agrochemistry
- 雑誌
- Agricultural and Biological Chemistry (ISSN:00021369)
- 巻号頁・発行日
- vol.48, no.9, pp.2293-2300, 1984
- 被引用文献数
-
11
40
In survey studies on microbial accumulation of S-adenosyl-L-methionine (AdoMet), various yeasts were found to accumulate AdoMet intracellularly to a high concentration when they were grown in medium containing L-methionine. A group of sake yeasts (<i>Saccharomyces sake</i>) exhibited especially high accumulation. Of these yeasts, <i>S. sake</i> Kyokai No. 6 (K-6), which exhibited the highest accumulation, produced 12.6 μmol (5.03 mg) of AdoMet/ml broth. Almost all AdoMet produced was accumulated in cells, extracellular accumulation of AdoMet being very low. The maximum content of AdoMet of cells was 5.31 μmol (205 mg)/g dry cells. This was the highest value that had been reported. Methionine adenosyltransferase (EC 2.5.1.6) activity was significantly higher in this yeast compared to those in other yeasts tested. Ultraviolet photomicrographic studies on <i>S. sake</i> K-6 suggested that AdoMet was gradually accumulated in vacuoles with the passage of cultivation time.