著者

Ahmed ALI Yasmine ABOULEILA Sara AMER Rie FURUSHIMA Samy EMARA Sebastien EQUIS Yann COTTE Tsutomu MASUJIMA

出版者

The Japan Society for Analytical Chemistry

雑誌

Analytical Sciences (ISSN:09106340)

巻号頁・発行日

vol.32, no.2, pp.125-127, 2016-02-10 (Released:2016-02-10)

参考文献数

10

被引用文献数

12 38

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The locations and volumes of the contents of a single HepG2 cell were visualized under three-dimensional (3D) holographic and tomographic (HT) laser microscopy, colored by refractive index, not staining. After trapping the specific area of a target cell in a nanospray tip, quantification was performed by live single-cell mass spectrometry. Comparison of the HepG2 cells’ before and after 3D-HT images allowed the inference of the precise volume and original location of the trapped cell contents. The total amount of a trapped molecule was estimated. The images also revealed morphological changes in the cell structure caused by the manipulation.