- 著者
- Ahmed ALI Yasmine ABOULEILA Sara AMER Rie FURUSHIMA Samy EMARA Sebastien EQUIS Yann COTTE Tsutomu MASUJIMA
- 出版者
- The Japan Society for Analytical Chemistry
- 雑誌
- Analytical Sciences (ISSN:09106340)
- 巻号頁・発行日
- vol.32, no.2, pp.125-127, 2016-02-10 (Released:2016-02-10)
- 参考文献数
- 10
- 被引用文献数
- 12 40
The locations and volumes of the contents of a single HepG2 cell were visualized under three-dimensional (3D) holographic and tomographic (HT) laser microscopy, colored by refractive index, not staining. After trapping the specific area of a target cell in a nanospray tip, quantification was performed by live single-cell mass spectrometry. Comparison of the HepG2 cells’ before and after 3D-HT images allowed the inference of the precise volume and original location of the trapped cell contents. The total amount of a trapped molecule was estimated. The images also revealed morphological changes in the cell structure caused by the manipulation.
1 0 0 0 OA Fluorescence Probing Live Single-cell Mass Spectrometry for Direct Analysis of Organelle Metabolism
- 著者
- Tsuyoshi ESAKI Tsutomu MASUJIMA
- 出版者
- (社)日本分析化学会
- 雑誌
- Analytical Sciences (ISSN:09106340)
- 巻号頁・発行日
- vol.31, no.12, pp.1211-1213, 2015-12-10 (Released:2015-12-10)
- 参考文献数
- 12
- 被引用文献数
- 1 26
Mitochondria in a live HepG2 cell were visualized with a fluorescent probe to specify their location and state in a living cell. Then, mitochondria were selectively captured with a nanospray tip under fluorescence microscope, and thousands of small molecular peaks were revealed and unique steroids specific to mitochondria were also found. This fluorescence imaging combined with live single-cell mass spectrometry opens the door to the analysis of site- and state-specific molecular detection to elucidate precise molecular mechanisms at the single-cell and organelle level.