著者

Yasuyuki Fujita Atsuhiko Kawamoto

出版者

The Editorial Committee of Annals of Vascular Diseases

雑誌

Annals of Vascular Diseases (ISSN:1881641X)

巻号頁・発行日

vol.15, no.4, pp.241-252, 2022-12-25 (Released:2022-12-25)

参考文献数

62

被引用文献数

2

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CD34 is a cell surface marker, which is expressed in various somatic stem/progenitor cells such as bone marrow (BM)-derived hematopoietic stem cells and endothelial progenitor cells (EPCs), skeletal muscle satellite cells, epithelial hair follicle stem cells, and adipose tissue mesenchymal stem cells. CD34+ cells in BM and peripheral blood are known as a rich source of EPCs. Thus, vascular regeneration therapy using granulocyte colony stimulating factor (G-CSF) mobilized- or BM CD34+ cells has been carried out in patients with various vascular diseases such as chronic severe lower limb ischemia, acute myocardial infarction, refractory angina, ischemic cardiomyopathy, and dilated cardiomyopathy as well as ischemic stroke. Pilot and randomized clinical trials demonstrated the safety, feasibility, and effectiveness of the CD34+ cell therapy in peripheral arterial, cardiovascular, and cerebrovascular diseases. This review provides an overview of the preclinical and clinical reports of CD34+ cell therapy for vascular regeneration.

著者

藤田 恭介 Yasuyuki FUJITA 帝京科学大学

出版者

帝京科学大学

雑誌

帝京科学大学紀要 (ISSN:18800580)

巻号頁・発行日

vol.9, pp.133-137, 2013

著者

Asuka SUTO Masahiro YAMASAKI Yukari TAKASAKI Yasuyuki FUJITA Riichiro ABE Hiroshi SHIMIZU Hiroshi OHTA Mitsuyoshi TAKIGUCHI

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.13-0098, (Released:2013-07-16)

被引用文献数

2 7

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Due to the lack of a gold standard method in canine lipoprotein analysis, it is unclear whether canine high-density lipoprotein (HDL) and low-density lipoprotein (LDL) can be accurately evaluated by the lipoprotein analysis methods used for dogs. This study investigated whether the ultracentrifugation-precipitation (U-P) method was suitable as a gold standard method for analyzing canine lipoprotein. First, the U-P method was compared with a gel permeation high performance liquid chromatography system (GP-HPLC). The concentrations of canine HDL-cholesterol (HDL-C) and LDL-cholesterol (LDL-C) determined by the U-P method correlated closely with those determined by GP-HPLC. However, the canine HDL-C concentration determined by the U-P method was lower than that by GP-HPLC, and the canine LDL-C concentration by the U-P method was higher than that by GP-HPLC. This study showed that some of the canine HDL could be precipitated with heparin manganese chloride solution. Second, the [HDL] and [LDL] fractions separated by the U-P method were analyzed by LC-MS/MS. The [HDL] fraction was found to contain only apolipoprotein A-I, which is an apolipoprotein of HDL, whereas the [LDL] fraction contained both apolipoprotein A-I and apolipoprotein B-100, which is an apolipoprotein of LDL. This data showed that a certain lipoprotein that includes apolipoprotein A-I might precipitate with canine LDL by use of heparin manganese chloride solution. These results indicated that the U-P method is not currently a gold standard method for analyzing canine lipoproteins.