著者

Ryota Yasui Ayaka Washizaki Yuko Furihata Tetsuro Yonesaki Yuichi Otsuka

出版者

The Genetics Society of Japan

雑誌

Genes & Genetic Systems (ISSN:13417568)

巻号頁・発行日

vol.89, no.2, pp.51-60, 2014-04-01 (Released:2014-09-13)

参考文献数

54

被引用文献数

1 10

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Bacteria have a variety of resistance mechanisms for surviving bacteriophage infections. Here, we describe a novel anti-phage mechanism in Escherichia coli. Cells harboring a plasmid with the genes abpA and abpB, formerly yfjL and yfjK, blocked the propagation of bacteriophages belonging to three families: T4, T2, T7 and λ phages. Both genes were necessary for the inhibition of phage propagation, and deletion of either chromosomal gene resulted in a 20% increase of progeny compared to wild-type cells. Neither overexpression nor deficiency of AbpA and AbpB had any apparent effect on E. coli growth. We isolated seven suppressor mutants of T4 phage that grew weakly on cells overexpressing AbpA and AbpB, and found that their mutations were all located in gene 41, which encodes a replicative DNA helicase that is essential for DNA replication. Furthermore, we demonstrated that AbpA and AbpB inhibited DNA replication and late gene expression of T4 phage. Similarly, DNA replication of T7 and λ phages was also inhibited by AbpA and AbpB. These results strongly suggest that E. coli AbpA and AbpB target DNA replication of phages to block their propagation.

著者

大塚 祐一 Yuichi Otsuka

雑誌

麗澤学際ジャーナル = Reitaku Journal of Interdisciplinary Studies (ISSN:21895333)

巻号頁・発行日

vol.25, pp.81-93, 2017-03-10

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In previous times, Japanese companies were regarded as a kind of community (Gemeinschaft). The Japanese management style which respects harmony and cooperation supported the growth of companies from the period of high economic growth to the 1980s. Generally, in Japanese management theory, the value of collectivism as an ideology is emphasized. Apart from this, a new view of corporate-community based on Aristotle philosophy (virtue ethics) or communitarian-thinking appeared after the 1990s. The purpose of this paper is to clarify the characteristics of each concept of corporate-community and to examine the differences between them.