著者

Akira Iwase Arika Takebayashi Yuki Aoi David S Favero Shunsuke Watanabe Mitsunori Seo Hiroyuki Kasahara Keiko Sugimoto

出版者

Japanese Society for Plant Biotechnology

雑誌

Plant Biotechnology (ISSN:13424580)

巻号頁・発行日

vol.39, no.1, pp.51-58, 2022-03-25 (Released:2022-03-25)

参考文献数

38

被引用文献数

4

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4-Phenylbutyric acid (4PBA) is utilized as a drug to treat urea cycle disorders and is also being studied as a potential anticancer drug that acts via its histone deacetylase (HDAC) inhibitor activity. During a search to find small molecules that affect plant regeneration in Arabidopsis, we found that 4PBA treatment promotes this process by mimicking the effect of exogenous auxin. Specifically, plant tissue culture experiments revealed that a medium containing 4PBA enhances callus formation and subsequent shoot regeneration. Analyses with auxin-responsive or cytokinin-responsive marker lines demonstrated that 4PBA specifically enhances AUXIN RESPONSE FACTOR (ARF)-dependent auxin responses. Our western blot analyses showed that 4PBA treatment does not enhance histone acetylation in Arabidopsis, in contrast to butyric acid and trichostatin A, other chemicals often used as HDAC inhibitors, suggesting this mechanism of action does not explain the observed effect of 4PBA on regeneration. Finally, mass spectroscopic analysis and genetic approaches uncovered that 4PBA in Arabidopsis plants is converted to phenylacetic acid (PAA), a known natural auxin, in a manner independent of peroxisomal IBR3-related β-oxidation. This study demonstrates that 4PBA application promotes regeneration in explants via its auxin activity and has potential applications to not only plant tissue culture engineering but also research on the plant β-oxidation pathway.

著者

James H.P. RICE Naoto TSUJII Yuichi TAKASE Akira EJIRI Osamu WATANABE Hibiki YAMAZAKI Yi PENG Kotaro IWASAKI Yuki AOI Yongtae KO Kyohei MATSUZAKI Yuki OSAWA

出版者

The Japan Society of Plasma Science and Nuclear Fusion Research

雑誌

Plasma and Fusion Research (ISSN:18806821)

巻号頁・発行日

vol.15, pp.2402009, 2020-04-06 (Released:2020-05-08)

参考文献数

13

被引用文献数

4

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A new Langmuir probe has been designed and installed in TST-2 for measurements of Scrape-Off Layer plasmas (SOL). Non-inductive current drive is considered essential for spherical tokamak reactors. It has previously been shown that a large amount of injected Lower Hybrid Wave (LHW) power is lost in the SOL [1]. A full density profile of SOL conditions is necessary to accurately simulate the propagation of LHW in TST-2. A new probe was designed for durability, larger signal and Mach probe measurements. The new probe has been installed in TST-2 and results have been obtained. Temperature measurements show Te = 30 - 50 eV during RF injection and <10 eV otherwise. Density measurements show ne = 2.0 × 1015 m−3 and 1.5 × 1016 m−3 during flat-top RF power injection from Outboard- and Top-launch antennas, respectively. This is above the cut-off density for the 200 MHz LHW (5 × 1014 m−3) in TST-2, thus LHW can propagate through SOL plasma.

著者

Naoto TSUJII Yusuke YOSHIDA Yuichi TAKASE Akira EJIRI Osamu WATANABE Hibiki YAMAZAKI Yi PENG Kotaro IWASAKI Yuki AOI Yongtae KO Kyohei MATSUZAKI James H.P. RICE Yuki OSAWA

出版者

The Japan Society of Plasma Science and Nuclear Fusion Research

雑誌

Plasma and Fusion Research (ISSN:18806821)

巻号頁・発行日

vol.15, pp.2402010, 2020-04-06 (Released:2020-05-08)

参考文献数

13

被引用文献数

2 4

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Removal of the central solenoid is considered essential to realize a spherical tokamak fusion reactor, but non-inductive plasma start-up is a challenge. Start-up using lower-hybrid (LH) waves has been studied on the TST-2 spherical tokamak at the University of Tokyo. The equilibrium poloidal field is believed to be generated mostly by the wave driven fast electrons, which are highly non-thermal and have large orbit excursions from the flux surfaces due to low plasma current. Such an equilibrium can be qualitatively different from the Grad-Shafranov equilibrium routinely used for internal magnetic field reconstruction in a tokamak. In this work, the effect of fast electrons on the MHD equilibrium was investigated by considering the equilibrium solution of the hybrid-MHD model [Y. Todo and A. Bierwage, Plasma Fusion Res. 9, 3403068]. The fast electron distribution function was estimated using a LH current drive simulation based on ray-tracing and an orbit-averaged Fokker-Planck solver. The equilibrium solution of the hybrid-MHD model was successfully fitted to the magnetic and kinetic measurements. The resulting poloidal flux function was more skewed towards the outboard side when fast electrons were introduced, which was more consistent with the density profile measured by the Thomson scattering diagnostic.