著者

桐生 浩希

出版者

埼玉大学大学院理工学研究科

雑誌

平成19年度環境制御システムコース修士論文発表会要旨集

巻号頁・発行日

vol.12, pp.17-20, 2008

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The prevalence of Japanese cedar pollinosis has been increased. Japanese cedar pollen (JCP), which contains two allergens Cry j 1 and Cry j 2, belongs to coarse particles (particle diameter >2 μm). Some researchers showed that respirable-sized particles containing pollen allergen (particle diameter <2 μm) may be resuspended after light rainfall in the atmosphere. These particles may cause pollen asthma, however, release mechanisms of them are still uncertain. Therefore, it is important to examine release behaviour of Cry j 1 and Cry j 2 from JCP. The aim of this study is to found respirable-sized particles containing Cry j 1 and Cry j 2 (Cry j 1, Cry j 2 particles) in the atmosphere, to examine elution phenomenon of Cry j 1 from JCP and JCP burst followed by Cry j 2 release. Cry j 1 and Cry j 2 particles were collected size-selectively and were visualized by immunofluorescence technique. Consequently, Cry j 1 and Cry j 2 particles can be confirmed to resuspend in the atmosphere. The original solutions (OS) and the eluted solutions (ES) were prepared from mixture of JCP and artificial rain solution (ARS). The percentage of Cry j 1 concentration of ES to that of OS has already been exceeded 50 % within 1 hour after mixing. In contrast, pollen exposed to humidified air wasn’t burst. However, it is suggested that pollen burst with rainfall is due to osmotic pressure by rainfall. As a result, it was supposed that Cry j 1 and Cry j 2 particles might be generated from JCP which contacts rainfall. We propose to convert from pollen information to allergen information and to recommend avoidance of pollen asthma.