著者

王 青躍 栗原 幸大 桐生 浩希 坂本 和彦 三輪 誠 内山 巌雄

出版者

日本エアロゾル学会

雑誌

エアロゾル研究 = Journal of aerosol research (ISSN:09122834)

巻号頁・発行日

vol.23, no.2, pp.120-126, 2008-06-20

被引用文献数

3

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In this study, we focus on the investigation of the airborne behavior of respirable particulate matters containing Japanese cedar pollen allergen Cry j 1 exfoliated from the Ubisch bodies on the pollen surfaces and Cry j 2 comprised in the starch granules within the pollen. The airborne Japanese cedar pollens were counted, and at the same time, the Cry j 1 and Cry j 2 particles were collected and determined in different particle sizes at the urban site (Cooperative Research Center of Saitama University) during daily sampling periods of the most severe pollination season of FY 2005 in Saitama, Japan.<BR>Morphological observation of Ubisch bodies exfoliated from pollen and ruptured pollens was performed with a scanning electron microscope. Moreover, the existing evidences of visualized Cry j 1 and Cry j 2 allergen particles below 1.1&mu;m were also sensitively found by an immunofluorescence technique with a fluorescence microscope. Simultaneously, the high concentrations of Cry j 1 and/or Cry j 2 were determined in the fine particle sizes below 1.1&mu;m. It was suggested that airborne respirable particulate matters containing Cry j 1 and Cry j 2 play a key role in the increasing incidence of asthma in the lower respiratory tract. Furthermore, although the release mechanisms of Cry j 1 and a Cry j 2 particles may be differed, the daily variation delays in the peaks of Cry j 1 (or Cry j 2) concentrations compared to the peaks of airborne pollen counts were observed since the fine particles were suspended in the atmosphere over a longer period of time after released. It is important to observe and determine the size distribution of airborne Japanese cedar pollen allergen particles in the long-term sampling research continually, and to elucidate the allergen particle release mechanisms. In the future, we should investigate the chemical, physical and biomedical combination effects on Japanese cedar pollen allergens, and have to consider proposing a new forecast informational system of the Japanese cedar pollen and their allergen particle variations.

著者

王 青躍 栗原 幸大 桐生 浩希 坂本 和彦 三輪 誠 内山 巌雄

出版者

日本エアロゾル学会

雑誌

エアロゾル研究 (ISSN:09122834)

巻号頁・発行日

vol.23, no.2, pp.120-126, 2008-06-20 (Released:2008-06-25)

参考文献数

22

被引用文献数

2

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In this study, we focus on the investigation of the airborne behavior of respirable particulate matters containing Japanese cedar pollen allergen Cry j 1 exfoliated from the Ubisch bodies on the pollen surfaces and Cry j 2 comprised in the starch granules within the pollen. The airborne Japanese cedar pollens were counted, and at the same time, the Cry j 1 and Cry j 2 particles were collected and determined in different particle sizes at the urban site (Cooperative Research Center of Saitama University) during daily sampling periods of the most severe pollination season of FY 2005 in Saitama, Japan.Morphological observation of Ubisch bodies exfoliated from pollen and ruptured pollens was performed with a scanning electron microscope. Moreover, the existing evidences of visualized Cry j 1 and Cry j 2 allergen particles below 1.1μm were also sensitively found by an immunofluorescence technique with a fluorescence microscope. Simultaneously, the high concentrations of Cry j 1 and/or Cry j 2 were determined in the fine particle sizes below 1.1μm. It was suggested that airborne respirable particulate matters containing Cry j 1 and Cry j 2 play a key role in the increasing incidence of asthma in the lower respiratory tract. Furthermore, although the release mechanisms of Cry j 1 and a Cry j 2 particles may be differed, the daily variation delays in the peaks of Cry j 1 (or Cry j 2) concentrations compared to the peaks of airborne pollen counts were observed since the fine particles were suspended in the atmosphere over a longer period of time after released. It is important to observe and determine the size distribution of airborne Japanese cedar pollen allergen particles in the long-term sampling research continually, and to elucidate the allergen particle release mechanisms. In the future, we should investigate the chemical, physical and biomedical combination effects on Japanese cedar pollen allergens, and have to consider proposing a new forecast informational system of the Japanese cedar pollen and their allergen particle variations.

著者

桐生 浩希

出版者

埼玉大学大学院理工学研究科

雑誌

平成19年度環境制御システムコース修士論文発表会要旨集

巻号頁・発行日

vol.12, pp.17-20, 2008

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The prevalence of Japanese cedar pollinosis has been increased. Japanese cedar pollen (JCP), which contains two allergens Cry j 1 and Cry j 2, belongs to coarse particles (particle diameter >2 μm). Some researchers showed that respirable-sized particles containing pollen allergen (particle diameter <2 μm) may be resuspended after light rainfall in the atmosphere. These particles may cause pollen asthma, however, release mechanisms of them are still uncertain. Therefore, it is important to examine release behaviour of Cry j 1 and Cry j 2 from JCP. The aim of this study is to found respirable-sized particles containing Cry j 1 and Cry j 2 (Cry j 1, Cry j 2 particles) in the atmosphere, to examine elution phenomenon of Cry j 1 from JCP and JCP burst followed by Cry j 2 release. Cry j 1 and Cry j 2 particles were collected size-selectively and were visualized by immunofluorescence technique. Consequently, Cry j 1 and Cry j 2 particles can be confirmed to resuspend in the atmosphere. The original solutions (OS) and the eluted solutions (ES) were prepared from mixture of JCP and artificial rain solution (ARS). The percentage of Cry j 1 concentration of ES to that of OS has already been exceeded 50 % within 1 hour after mixing. In contrast, pollen exposed to humidified air wasn’t burst. However, it is suggested that pollen burst with rainfall is due to osmotic pressure by rainfall. As a result, it was supposed that Cry j 1 and Cry j 2 particles might be generated from JCP which contacts rainfall. We propose to convert from pollen information to allergen information and to recommend avoidance of pollen asthma.