- 著者
-
上田 明良
遠田 暢男
- 出版者
- 応用森林学会
- 雑誌
- 日本林学会関西支部論文集 (ISSN:09182268)
- 巻号頁・発行日
- vol.5, pp.143-144, 1996-03-25 (Released:2018-01-23)
A simple method of rearing of the Japanese pine sawyer, Monochamus alternatus Hope (Coleoptera: Cerambycidae) on an artificial diet is proposed. The diet is composed of a commercially available artificial diet used for the silkworm, Bombix mori (Silkmate 3M(R); Nippon Nosan Kogyo Co.), milled fresh inner bark of Japanese red pine, dried yeast and tap water. The masses of each ingredient required for 150 beetles (=150 rearing bottles) are 2kg, 1kg, 20g and 1500ml, respectively. The bottles were autoclaved for 10 min. (1.2kg/cm^2, 120℃). In the case of inoculated first and second instar larvae from Kyoto and Ibaraki, Japan, these were reared at 25℃LD10:14 for five months after oviposition, chilled for two months at 10℃ constant darkness and then reared at 25℃LD16:8. One hundred and seventy five beetles from 210 inoculated larvae (84.8%) emerged an average 47.2 days after chilling.