- 著者
- Yamada Parida Ono Takako Shigemori Hideyuki Han Junkyu Isoda Hiroko
- 出版者
- Springer Science+Business Media B.V.
- 雑誌
- Cytotechnology (ISSN:09209069)
- 巻号頁・発行日
- vol.64, no.3, pp.349-356, 2012-05
- 被引用文献数
- 10
In this study, the anti-allergy potency of thirteen tannins isolated from the galls on buds of Carpinus tschonoskii (including two tannin derivatives) was investigated. RBL-2H3 (rat basophilic leukemia) cells were incubated with these compounds, and the release of β-hexosaminidase and cytotoxicity were measured. Of the thirteen tannins, tetragalloylglucose (2), pentagalloylglucose (3), casuarictin (4), and casuarinin (9) were the most potent inhibitors, and all the tannins showed no cytotoxic effect after 24 h of incubation. The results obtained suggest that tannins from C. tschonoskii are capable of inhibiting allergic reactions and may be useful for the treatment or prevention of type I allergic diseases.
- 著者
- Han Jun Kyu Akutsu Mitsuaki Talorete Terence P. N. Maekawa Takaaki Tanaka Toshiyuki Isoda Hiroko
- 出版者
- Springer
- 雑誌
- Cytotechnology (ISSN:09209069)
- 巻号頁・発行日
- vol.47, no.1-3, pp.89-96, 2005-01
- 被引用文献数
- 11 10
On the basis of transepithelial electrical resistance (TER) measurements, we found that capsaicin (100 μM)-treated human intestinal Caco-2 cells show a momentary increase in tight-junction (TJ) permeability (decrease in TER) followed by a complete recovery. We used proteome analysis to search for proteins that are associated with the recovery of TJ permeability in capsaicin-treated Caco-2 cells. A protein with a relative molecular mass of 14 kDa was found to be expressed more highly in capsaicin-treated cells than in nontreated cells. Mass spectrometry and sequence analyses revealed that the protein that is expressed significantly upon capsaicin treatment is the ribosomal protein P2; its cDNA sequence was identical to that found in the human genome database. An increase in the amount of cellular filamentous actin (F-actin) was shown after 8 h of incubation with capsaicin. It has been reported that P2 activates elongation factor 2, which stabilizes F-actin filaments, and that the depolymerization of F-actin is associated with the increase in TJ permeability (decrease in TER). Consequently, these results suggest that P2 plays an important role in the recovery of the TJ permeability in capsaicin-treated human intestinal cells.