著者

Ando Ho Kakuda Ken-ichi Nakayama Munetaka YOKOTO Kei-ichi

出版者

一般社団法人日本土壌肥料学会

雑誌

Soil science and plant nutrition (ISSN:00380768)

巻号頁・発行日

vol.46, no.1, pp.105-115, 2000-03-01

被引用文献数

2

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Due to high the labor cost, direct seeding is being gradually applied for crop establishment throughout Asia. Recently, the development of the so-called, "controlled release fertilizers" (CRFs) which requires only "one shot dose" of N, has enabled to reduce the cost of labor for the application of topdressed N. Thus, in order to limit pollution and cost, no-tillage direct-seeded lowland rice cultivation accompanied by the use of CRF is being recommended. To evaluate the effects of CRFs on the growth and yield of no-tillage direct-seeded rice, a field experiment was carried out in the north-eastern region of Japan in 1993 and 1994. Five N treatments were used: (1) ammonium sulfate (AS) band placement application with seeds at the time of seeding (AS1); (2) AS broadcast application after irrigation (AS2); (3) CRF (LP100) co-situs application at the time of seeding (L1); (4) CRF (LPS100) co-situs application at the time of seeding (L2); and (5) minus N (MN). The rate of basal N was 60 kg N ha^<-1>. Topdressed N in the AS1 and AS2 treatments was applied at 25 d before heading at the rate of 20 kg N ha^<-1>. The highest yield was obtained in the L1 treatment in both years. The number of grains per unit area was affected by the amount of N in the plants at heading. The percentage of filled grains in the L2 treatment was the lowest by about 15 to 20% among the treatments. The recovery percentages of basal N on July 20 were about 20 to 30% in the AS1, AS2, and L1 treatments, and only about 14% in the L2 treatment. About 75 to 80% of basal N was absorbed by the plants at harvest in the L1 and L2 treatments. On the other hand, the plants had absorbed only about 25 to 30% of the applied basal N in the AS1 and AS2 treatments at harvest. There was no difference in the N-recovery percentage (based on the amount of released N) between the L1 and L2 treatments. Nitrogen-use efficiency was not significantly different among the treatments. However, higher N-use efficiency was obtained in this experiment than in early studies in the tropics. The agronomic efficiency of CRFs was higher than that of ammonium sulfate.

著者

Priyadi Kahar Hadi Abdul Siagian Tiusbul H. Nisa Chatimatun Azizah Aulia Raihani Nilly Inubushi Kazuyuki

出版者

一般社団法人日本土壌肥料学会

雑誌

Soil Science and Plant Nutrition (ISSN:00380768)

巻号頁・発行日

vol.51, no.5, pp.689-691, 2005-09

被引用文献数

1

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A field experiment was carried out to elucidate the effect of chicken manure (CM) and effective microorganisms (EM) on the yield of corn and chemical and microbial properties of acidic wetland soils. A split-split plot experimental design with three replications was used in this research. The main plot consisted of two types of soil. The sub-plot was for the application of CM, while the sub-sub plot was for the application of EM. Sweet corn seeds were planted and cultivated until harvest. Above ground plant biomass and round of the stem of an ear of corn were observed. Soil samples were taken and analyzed for chemical and microbial properties. The results showed that the interaction between soil types and CM application affected the corn yield, while EM had no effect. Similarly, the chemical and microbial characteristics of soils used were affected by soil type and the application of CM, while the effect of EM was not clear.

著者

Gotoh Shigeyoshi Yamashita Keiichi

出版者

Informa UK Limited

雑誌

Soil Science and Plant Nutrition (ISSN:00380768)

巻号頁・発行日

vol.12, no.6, pp.24-32, 1966-11-27

著者

YAMZAKI Masatsugu ASAKAWA Susumu MURASE Jun KIMURA Makoto

出版者

一般社団法人日本土壌肥料学会

雑誌

Soil science and plant nutrition (ISSN:00380768)

巻号頁・発行日

vol.58, no.1, pp.11-23, 2012-02-01

参考文献数

40

---

Free-living fresh water Platyhelminthes except for the order Tricladida (planaria) are collectively called microturbellaria, most species of which are less than a few millimeters in length. The ecology and the fauna of microturbellarians in rice fields has not been clarified in detail since Okugawa (1932) reported the morphological and ecological characters of all microturbellarian species that had been observed in Japan. This might be because the observation of living microturbellarians is needed for their identification. To survey phylogenetic diversity of microturbellarians in ten Japanese rice paddy fields, after DNA was extracted from all microturbellarian individuals that were collected in each field, the composition of the amplified 18S rRNA gene fragments of each sample was surveyed by clone library analysis. We also collected Stenostomum species in some of the same fields, identified them on the basis of morphological characters, and checked whether the 18S rRNA fragments of these species were contained within those of microturbellarians that were obtained by clone library analysis. Twenty-eight types of 18S rRNA gene fragments of microturbellarians were isolated on the basis of the difference in the sequence, and they were divided into seven families of microturbellarians (Stenostomidae, Catenulidae, Typhloplanidae, Dalyellidae, Gyratricidae, Macrostomidae, and Microstomidae). We identified four Stenostomum species of Stenostomidae (Stenostomum grande Child 1902, Stenostomum simplex Kepner & Carter 1931, Stenostomum tuberculosum Nuttycombe & Waters 1938, Stenostomum saliens Kepner & Carter 1931) that had never been reported in rice fields. The sequences of 18S rRNA gene fragments of Stenostomidae in the floodwater around rice plants showed the highest diversity, and they were closely related to Stenostomum leucops (Duges 1828) O. Schmidt 1848, S. grande, S. simplex, S. tuberculosum, S. saliens, Stenostomum heebuktense Larsson & Willems 2010, and Rhynchoscolex simplex Leidy 1851. 18S rRNA gene fragments of Catenulidae and Typhloplanidae showed high diversity, and those of Microstomidae, Macrostomidae, Gyratricidae, and Dalyellidae were also retrieved. These molecular biological techniques revealed that many unidentified groups of microturbellarians inhabited rice fields.

著者

小林 理志 関 聡史 坂田 真一 松岡 誠一 藤井 広志

出版者

一般社団法人日本土壌肥料学会

雑誌

Soil science and plant nutrition (ISSN:00380768)

巻号頁・発行日

vol.48, no.3, pp.315-324, 2002-06-01

参考文献数

39

被引用文献数

1

---

The underground railway project progresses to relocate the operating railway on the surface in Yokohama city of Metropolitan area. Especially in soft ground with shallow cover, tunnel is excavated by shotcreting method, and various auxiliary measures and monitoring control system are employed because of the need to secure operating track during the excavation. In this project, an approximately 2km long section near Yokohama station, is being relocated to underground railway to enable through train service with the new subway line. The construction of underground relocation is being performed by shotcreting method as selected by considering the topography, geology, depth of cover, and adjacent structures in each section. This paper describes construction of the urban tunnel just beneath operating railway.

著者

Kojima Soichi

出版者

Taylor & Francis

雑誌

Soil Science and Plant Nutrition (ISSN:00380768)

巻号頁・発行日

vol.64, no.5, pp.616-622, 2018-08-06

被引用文献数

3

著者

Kushari D.P. Watanabe Iwao

出版者

一般社団法人日本土壌肥料学会

雑誌

Soil science and plant nutrition (ISSN:00380768)

巻号頁・発行日

vol.38, no.1, pp.65-73, 1992-03

被引用文献数

1

---

The growth of Azolla in the field is often limited by the phosphorous supply. To develop a screening technique for the ability of Azolla to grow and fix N_2 more efficiently under P-deficient conditions, water culture experiments were conducted under concentration-controlled conditions. Azolla was grown in a 60-liter bucket with a P concentration ranging from 0.02 to 0.06 ppm. At 0.03 ppm, Azolla became P-deficient. Under P-deficient condition, Azolla pinnata strains #5 and #7001 produced a larger amount of biomass and showed a lower tissue P concentration than A. microphylla #4018 and A. mexicana #2026. The P concentration in the culture medium decreased from 0.03 to 0.02 ppm during the growth of Azolla. A device for the supply of acid or alkaline and P solutions was designed to maintain a constant pH and P concentration in the water culture medium. Even when the P concentration was kept at 0.03-0.035 ppm, Azolla suffered from P deficiency. Under this condition, the A. microphylla, A. mexicana, A. filiculoides, and A. nilotica strains produced a smaller amount of biomass and showed a higher tissue P content than the A. pinnata strains. Growth responses of Azolla species under P concentration-limited conditions were, thus, similar to those under quantity-limited conditions. Quantity-limited water culture is more convenient for screening a large number of Azolla strains for their ability to grow under P-deficient conditions.

著者

TAKAGI Sei-ichi

出版者

一般社団法人日本土壌肥料学会

雑誌

Soil science and plant nutrition (ISSN:00380768)

巻号頁・発行日

vol.22, no.4, pp.423-433, 1976-12

被引用文献数

29

---

The root washings of water-cultured oat and rice (non-sterile) contained some sort of amphoteric, iron-solubilizing chelating (or complexing) compound(s), which could be separated into a "cationic fraction" by elution in a cation exchanger column with 1N NH_4OH. In oats, the release of the chelator into the washings greatly increased under iron-stress conditions. Under the same condition "iron-inefficient" rice plants also increased the release of the chelator, though to a much less extent than did the oats. Further examination of the "cationic fraction" revealed that this chelator may be a heat-stable, acid-hydrolyzable non-macromolecule of extraordinarily high polarity. In the absence of interfering ions, the chelator was able to solubilize hydrated Fe (III) oxide effectively within a range of pH 4 to 9. Its Fe-solubilizing action was inhibited by the presence of divalent metals, the extent being in the order, Cu>Co≧Zn>Mn>Ca (no inhibition).

著者

OMOTE Yushi TAKATA Yusuke

出版者

一般社団法人日本土壌肥料学会

雑誌

Soil science and plant nutrition (ISSN:00380768)

巻号頁・発行日

vol.60, no.4, pp.470-474, 2014-08

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To enhance the efficiency of soil information in the field survey, we developed the mobile application called "e-SoilMap" for creating a new user-oriented soil map. This application has the following three unique functions: annotatable map, data sharing over the cloud and offline support for allowing the user to create a high-value-added soil map in their Internet space. Both iOS and Android versions of the mobile application mainly consist of three key components: (1) a map manager, (2) a soil manager and (3) a user manager. The map manager is responsible for displaying background maps to the user. The soil manager displays a soil map and related soil information, which are provided by the National Institute for Agro-Environmental Sciences (NIAES), on the background maps. And the user manager is responsible for creating an annotatable map and sharing the user data in the cloud. The "e-SoilMap" provides soil information to users using location-based spatial query processing and allows the creation of annotatable pins on the soil map. The main target of this application was designed to be a wide variety of technical fields such as agronomic assessment, engineering applications, hydrology and hydrogeologic assessments, environmental assessment and policy decisions.

著者

Shtangeeva Irina Ayrault Sophie Jain Jinesh

出版者

一般社団法人日本土壌肥料学会

雑誌

Soil science and plant nutrition (ISSN:00380768)

巻号頁・発行日

vol.50, no.6, pp.877-883, 2004-12-01

参考文献数

26

---

Uptake of scandium (Sc) by wheat seedlings was studied during 6-d germination of wheat seeds in Sc supplemented medium and during 1-week growth of wheat seedlings germinated under normal conditions in soil amended with Sc. The most significant increase in Sc content in the seedlings was observed during germination of seeds in Sc supplemented medium. Transfer of the seedlings to normal soil resulted in a decrease of Sc level in upper plant parts, but Sc concentration in roots remained rather high. Scandium bioaccumulation caused variations in concentrations of Na, K, Ca, and Zn in different parts of the seedlings. The least variations were observed in leaves.

著者

Shtangeeva Irina Ayrault Sophie Jain Jinesh

出版者

一般社団法人日本土壌肥料学会

雑誌

Soil science and plant nutrition (ISSN:00380768)

巻号頁・発行日

vol.50, no.6, pp.877-883, 2004-12

---

Uptake of scandium (Sc) by wheat seedlings was studied during 6-d germination of wheat seeds in Sc supplemented medium and during 1-week growth of wheat seedlings germinated under normal conditions in soil amended with Sc. The most significant increase in Sc content in the seedlings was observed during germination of seeds in Sc supplemented medium. Transfer of the seedlings to normal soil resulted in a decrease of Sc level in upper plant parts, but Sc concentration in roots remained rather high. Scandium bioaccumulation caused variations in concentrations of Na, K, Ca, and Zn in different parts of the seedlings. The least variations were observed in leaves.

著者

HATANO Ryusuke IWANAGA Keiko OKAJIMA Hideo SAKUMA Toshio

出版者

一般社団法人日本土壌肥料学会

雑誌

Soil science and plant nutrition (ISSN:00380768)

巻号頁・発行日

vol.34, no.4, pp.535-546, 1988-12

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We studied the role of soil macropores in the promotion of root elongation. The roots of maize plants were grown in undisturbed soil columns 30 cm in diameter and 10 cm in height. The number of visible macropores with or without roots was counted in horizontal sections cut every 2-3 cm. The soils used were taken from the BC and C horizons of a Brown Lowland Soil, the BCg and Cg horizons of a Pseudogley and the B horizon of an Ordinary Andosol. The distribution patterns of the soil macropores varied with the soil types. Brown Lowland Soil was characterized by a large number of tubular macropores with a clustered distribution pattern. Ordinary Andosol was characterized by a small number of tubular macropores with a scattered distribution. Pseudogley was characterized by a linked distribution of interstitial macropores. The degree of clustering or linking of the macropores was more pronounced in the B horizons of both the Brown Lowland Soil and Pseudogley than in the C horizons. X^2 test revealed that the root elongation depended on the presence of macropores in each type of soil. Moreover, the distribution of the roots corresponded fairly well with the distribution of the macropores. The proportion of roots that elongated into macropores was higher in the soils with abundant clusters or linkages of macropores. To analyze this relationship, we introduced the concept of entropy as an index to characterize the distribution of macropores. The more pronounced the degree of clustering or linking of the macropores, the lower the value of entropy. The value of entropy, however, was also affected by the mean density of the macropores, and the larger the number of the macropores the higher the value of entropy. To eliminate the influence of the mean density, the ratio of the actual entropy (S') to the entropy (S) calculated on the assumption that all the macropores were distributed independently. The values of S'/S were low in the B horizons of the Brown Lowland Soil and Pseudogley while high in the Ordinary Andosol. The values of S'/S showed a strong negative correlation with the proportion of the roots penetrating the clustered or linked macropores. This fact suggests that the distribution patterns of the macropores may reflect the mechanical strength, bulk density, and other factors in the soil which hamper root elongation.

著者

Yoshinaga N. AOMINE Shigenori

出版者

一般社団法人日本土壌肥料学会

雑誌

Soil science and plant nutrition (ISSN:00380768)

巻号頁・発行日

vol.8, no.3, pp.114-121, 1962-05

被引用文献数

5

著者

ISHIKURI Shu SUWA Yuichi HATTORI Tsutomu

出版者

一般社団法人日本土壌肥料学会

雑誌

Soil science and plant nutrition (ISSN:00380768)

巻号頁・発行日

vol.30, no.2, pp.249-253, 1984-06

著者

Murakami Hiroharu Tsushima Seiya Kuroyanagi Yukiko Shishido Yoshihiro

出版者

一般社団法人日本土壌肥料学会

雑誌

Soil science and plant nutrition (ISSN:00380768)

巻号頁・発行日

vol.48, no.5, pp.685-691, 2002-10

被引用文献数

6

---

Relationships between the disease severity of clubroot caused by Plasmodiophora brassicae, the soil pH value and the concentration of exchangeable calcium associated with liming were investigated under controlled density of resting spores. Disease indices were lower in the plots treated with lime than in the control plots without lime application. The disease index was significantly lower when lime materials were mixed two weeks before sowing compared with four weeks before sowing. The reduction rate of the disease index was larger for a concentration of 2.0 g kg^-1 than 1.0 g kg^-1 of lime in soil. The density of the resting spores in soil at the time of sowing was significantly reduced by liming. The reduction rate was 17-31% for calcium cyanamide, 12-29% for dolomite, and 20-39% for calcium carbonate compared with the control plot. It was suggested that the disease severity was influenced by both the soil pH and the content of exchangeable calcium in soil based on the analysis of covariance.

著者

Katoh Masahiko Murase Jun Hayashi Motoki Matsuya Kazuo Kimura Makoto

出版者

一般社団法人日本土壌肥料学会

雑誌

Soil science and plant nutrition (ISSN:00380768)

巻号頁・発行日

vol.50, no.5, pp.721-729, 2004-10

被引用文献数

6

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To estimate the impact of water percolation on the nutrient status in paddy fields, the seasonal variations of the concentrations of cations, anions, inorganic carbon (IC), and of dissolved organic carbon (DOC) in percolating water that was collected from just below the plow layer (PW-13) and from drainage pipes at the 40 cm depth (PW-40), as well as in irrigation water were measured in an irrigated paddy field. Total amounts of Ca, Mg, K, Fe, and Mn leached from PW-13 during the period of rice cultivation were estimated to range from about 390 to 770, 65 to 130, 33 to 66, 340 to 680, and 44 to 87 kg ha^<-1>, respectively. Amounts of losses that were estimated from the differences between the input by irrigation water and the output by percolation water from the plow layer corresponded to 11 to 26, 22 to 47, 5.9 to 12, and 13 to 26% of exchangeable Ca and Mg, amorphous Fe, and easily reducible Mn in the plow layer, respectively. The concentrations of Ca, Mg, K, Fe, and Mn in PW-13 were higher than those in PW-40. The amounts of these nutrients that were retained in the subsoil between the 13 cm and 40 cm soil depth corresponded to 83, 86, 61, 99, and 89% of the amounts that percolated from the plow layer, respectively. Total amounts of IC and DOC that percolated from the plow layer ranged from 750 to 1,500 and 85 to 170 kg-C ha^<-1>, which corresponded to 5.0 to 10.0% and 0.6 to 1.1% of the total carbon content in the plow layer, respectively. Eighty eight % of IC in the percolating water from the plow layer was also retained in the subsoil.

著者

Tokuda Shin-ichi Hayatsu Masahito

出版者

一般社団法人日本土壌肥料学会

雑誌

Soil science and plant nutrition (ISSN:00380768)

巻号頁・発行日

vol.50, no.3, pp.365-374, 2004-06

被引用文献数

7

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Nitrous oxide (N_2O) is one of the main greenhouse gases, and accurate estimation of the N_2O flux from fertilized arable land is required. It is known that acidic tea field soil displays a higher N_2O production activity than neutral arable soil and that tea fields could be a major source of N_2O. Therefore, N_2O fluxes from four plots (Std, 2N, 2Ca and -Ca plots) in a tea field that had been subjected to different conditions of fertilizer management were measured using the closed chamber method over a period of two years, and the relationships between the N_2O flux and soil environmental factors were analyzed. The amounts of nitrogen fertilizer and liming material (dolomite) applied to the Std plot were 600 kg N ha^<-1> y^<-1> and 1,500 kg ha^<-1> y^<-1>, respectively. The amount of nitrogen fertilizer applied to the 2N plot was two-times larger than that applied to the Std plot and corresponded to the conventional level in Japanese tea fields. The soil was acidified due to heavy nitrogen fertilization in the 2N plot. The 2Ca plot was amended with two-times the amount of liming material of the Std plot and in the -Ca plot no liming material was applied. There were significant differences among the N_2O fluxes from the plots, and the highest value of N_2O flux was 8.785 mg N m^<-2> h^<-1> in the 2N plot. Annual emission rate and emission factor of N_2O in the 2N plot were 25.22kg N_2O-N ha^<-1> and 2.10%, respectively. Both long-term heavy nitrogen fertilization and subsequent soil acidification possibly enhanced the N_2O flux from the tea field. The N_2O flux from the tea field showed temporal variations, namely the N_2O flux was relatively low from December to March possibly due to the low soil temperature and it increased gradually after March as the soil temperature increased over 10℃. The N_2O flux reached the first major peak in July, decreased transiently in August presumably due to the drying of soil, increased again and reached the second peak in September or October, and then decreased after November. Multiple linear regression analysis of the relationships between the N_2O flux and soil environmental factors indicated that the N_2O production activity was significantly related to the N_2O flux from the tea field. The N_2O production activity showed temporal variations corresponding to the temporal variations of the N_2O flux.

著者

Khan Harunor Rashid Rahman Shafiur Hussain Mohammad Sultan Adachi Tadashi

出版者

一般社団法人日本土壌肥料学会

雑誌

Soil science and plant nutrition (ISSN:00380768)

巻号頁・発行日

vol.40, no.2, pp.231-242, 1994-06

被引用文献数

3

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A pot experiment was conducted in relation to the growth, yield, and nutrition of rice plants cultivated in an acid sulfate soil treated with chemical fertilizers, basic slag, lime (CaCO_3) MnO_2, and leaching. The soil showed a low pH (4.0-4.5 : field), high ECe (0.7-1.6 Sm^<-1>) and consisted of a sulfuric horizon within the 1.2 m depth of the soil surface. Maximum growth and yield of rice cultivated in the soil were attained by the application of leaching plus basic slag treatment at the rate of 12.5 g kg^<-1>. The other treatments also resulted in a significantly (p <__- 0.05) improved performance compared with the control treatment (where no amendment was applied). Leaching, basic slag, MnO_2, and lime as single amendment brought about a grain yield increase of 500-900%, while leaching plus various doses of basic slag and lime resulted in an increase of grain yield of more than 1,500% over the control treatment. The highest N content of rice was obtained by the application of leaching plus basic slag (12.5 g kg^<-1>) and leaching plus lime (7.5 g kg^<-1>) treatments. The highest P, Ca, and Mg contents were obtained by the application of leaching plus lime treatment at the rate of 7.5 g kg^<-1> and the maximum content of K was recorded after basic slag treatment at the rate of 7.5 g kg^<-1>. Leaching, lime and basic slag treatments led to a decrease of the Fe, Mn, and Zn concentrations in the plants and soil solutions (leachates). The addition of lime, basic slag, or MnO_2 exerted a significant effect (p <__- 0.05) on the decrease of the S content, increase of the soil solution pH, and optimization of some element concentrations in the plants and soil solutions.

著者

Aoyama Masakazu Nagumo Toshiyuki

出版者

一般社団法人日本土壌肥料学会

雑誌

Soil science and plant nutrition (ISSN:00380768)

巻号頁・発行日

vol.42, no.4, pp.821-831, 1996-12

被引用文献数

2

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The size of the microbial biomass and dehydrogenase activity were measured in air-dried and rewetted apple orchard surface soils with accumulation of Cu, Pb, and As due to the application of Bordeaux mixtures and lead arsenate. The largest amounts of total Cu, Pb, and As found in the soils used were 1, 108, 1, 271, and 209 mg kg^<-1> soil, respectively. The amounts of 0.1 M HCl-extractable heavy metals were strongly correlated with the total amounts, while those of 0.1 M CaCl_2-extractable heavy metals, except for As, increased significantly with decreasing soil pH. The amounts of microbial biomass C and N, expressed on a soil organic C and total N basis, respectively, were each negatively correlated with the amounts of total and 0.1 M HCl-extractable Cu. On the other hand, the dehydrogenase activity was not affected by the amounts of total and 0.1 M HCl-extractable heavy metals, and was negatively correlated with the amount of 0.1 M CaCl_2-extractable Cu and positively with the soil pH. Higher significant correlations were observed when the dehydrogenase activity was calculated per unit of soil organic C. Thus the microbial biomass was adversely affected by the slightly soluble fractions of Cu accumulated in apple orchard soils, whereas the dehydrogenase activity was affected by the water-soluble and exchangeable Cu of which amount depended on the soil pH. It is suggested that the microbial biomass and dehydrogenase activity expressed on a soil organic matter basis could become useful indicators for assessing the effects of heavy metals on the size and activity of the microbial biomass in soils differing in organic matter contents

著者

Kim Hoyeun Fujiwara Toru Hayashi Hiroaki Chino Mitsuo

出版者

一般社団法人日本土壌肥料学会

雑誌

Soil science and plant nutrition (ISSN:00380768)

巻号頁・発行日

vol.43, pp.1119-1123, 1997-12

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Composition of seed storage proteins in in vitro cultured cotyledons is known to be affected by exogenous application of abscisic acid (ABA) and sulfur deficiency. In this paper, we analyzed effects of exogenous ABA on regulation of soybean seed storage protein accumulation in relation to that of sulfur deficiency. When exogenous ABA was applied at 10 μM and 100 μM, accumulation of the β subunit of β-conglycinin, a major seed storage protein, increased, whereas that of glycinin, another major seed storage protein, decreased. Free sulfate concentrations in cotyledons were not significantly affected by application of 10 μM ABA, but doubled by application of 100 μM ABA. As we have previously shown that concentrations of O-acetyl-L-serine (OAS), a precursor of cysteine biosynthesis, in in vitro cultured soybean cotyledons increased under sulfur deficiency, effects of exogenous ABA application on OAS concentration in soybean cotyledons were determined. The concentrations of OAS increased in accordance with the increase of exogenous ABA concentrations. These results suggested that exogenous application of ABA induces changes in soybean storage protein composition through the increase of OAS concentration.