著者

中潟 直己

出版者

Japanese Association for Laboratory Animal Science

雑誌

Experimental Animals (ISSN:00075124)

巻号頁・発行日

vol.43, no.1, pp.11-18, 1994-01-01 (Released:2010-08-25)

参考文献数

44

被引用文献数

4 6

---

Embryos, oocytes and spermatozoa of mice could be successfully preserved at -1961 by simple freezing methods. The survival rate of frozen embryos was very high at thawing and they developed into normal young after embryo transfer. In gametes, the cryopreserved oocytes could be fertilized in vitro by fresh spermatozoa and the cryopreserved spermatozoa could fertilize fresh oocytes. Moreover, the cryopreserved oocytes could be fertilized by the cryopreserved spermatozoa and the embryos obtained by in vitro fertilization between cryopreserved gametes could develop into normal, live young after embryo transfer. In the future, if not only the embryos but also the oocytes and spermatozoa of many mouse strains are frozen, normal young can be produced from cryopreserved embryos and gametes.