著者

及川 栄二 鈴木 直人 大越 哲也 海谷 幸利 福岡 雅人 前野 正夫 大塚 吉兵衛

出版者

硬組織再生生物学会

雑誌

日本硬組織研究技術学会雑誌 (ISSN:13405179)

巻号頁・発行日

vol.3, no.2, pp.85-92, 1994-10-29

被引用文献数

12

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An attempt was made to compare cell growth and three different phosphatase activities between human alveolar bone-derived (HAB) cells from young (13-22 years old) patients and those from older (55-73 years old) patients. HAB cells growing out of individual bone fragments were harvested separately, and subcultured up to the 3rd passage. The growth and phosphatase activities of HAB cells from each individual were detemined on days 8, 24 and 40 of culture after seeding. The enzymes determined were alkaline phosphatase (ALPase), acid phosphatase (ACPase) and tartrateresistant acid phosphatase (TRACPase). Morphologically, the HAB cells used in this study were spindle-shaped in spite of aging. The individual data obtained were averaged within each age group, and the means (SD) of the two groups were compared. Cell growth showed a logarithmic pattern from day 3 in both age groups. The HAB cells from the young age groupreached confluence on day 11 of culture, whereas those from the older age group did so on day 21. The replication capacity of HAB cells from the young group was greater than that of cells from the older group. ALPase activity in both groups increased from day 8 to reach a maximum on day 24 of culture, followed by a decrease on day 40. On day 24, HAB cells from the young group showed in approximately 2.2-fold higher ALPase activity than those from the older group. On the other hand, no significant differences were observed in ACPase and TRACPase activities between the two groups between days 8 and 40.