著者
永井 教之 村田 勝 辻極 秀次 井上 正久 長塚 仁 中野 敬介 赤木 巧 衣田 圭宏 完山 学 秦 春林 張 紹全 此内 浩信 韓 松 黄 炳珍 譚 軍 劉 桂需 Gomah Atia 日比 一光 Noriyuki Nagai Masaru Murata Hidetsugu Tsujigiwa Masahisa Inoue Hitoshi Nagatsuka Keisuke Nakano Takumi Akagi Yoshihiro Kinuta Manabu Kanyama Chun-Lin Qin Shao-Quan Zhang Hironobu Konouchi Song Han Bing-Zhen Huang Jun Tan Gui-Ru Liu Atia Gomah kazumitsu Hibi 岡山大学歯学部口腔病理学講座 岡山大学歯学部口腔病理学講座 岡山大学歯学部口腔病理学講座 岡山大学歯学部口腔病理学講座 岡山大学歯学部口腔病理学講座 岡山大学歯学部口腔病理学講座 岡山大学歯学部口腔病理学講座 岡山大学歯学部口腔病理学講座 岡山大学歯学部口腔病理学講座 岡山大学歯学部口腔病理学講座 岡山大学歯学部口腔病理学講座 岡山大学歯学部口腔病理学講座 岡山大学歯学部口腔病理学講座 岡山大学歯学部口腔病理学講座 岡山大学歯学部口腔病理学講座 岡山大学歯学部口腔病理学講座 岡山大学歯学部口腔病理学講座 岡山大学歯学部口腔病理学講座 Department of Oral Pathology Okayama University Dental School Department of Oral Pathology Okayama University Dental School Department of Oral Pathology Okayama University Dental School Department of Oral Pathology Okayama University Dental School Department of Oral Pathology Okayama University Dental School Department of Oral Pathology Okayama University Dental School Department of Oral Pathology Okayama University Dental School Department of Oral Pathology Okayama University Dental School Department of Oral Pathology Okayama University Dental School Department of Oral Pathology Okayama University Dental School Department of Oral Pathology Okayama University Dental School Department of Oral Pathology Okayama University Dental School Department of Oral Pathology Okayama University Dental School Department of Oral Pathology Okayama University Dental School Department of Oral Pathology Okayama University Dental School Department of Oral Pathology Okayama University Dental School Department of Oral Pathology Okayama University Dental School Department of Oral Pathology Okayama University Dental School
雑誌
日本硬組織研究技術学会雑誌 = Journal of hard tissue biology (ISSN:13405179)
巻号頁・発行日
vol.5, no.2, pp.163-173, 1996-07-10
参考文献数
32
被引用文献数
3

A modofied method was established to extracted BMP from bovine bone. SDS-PAGE and Westen blotting were apllied to analize the components of this partially purified BMP. In addition the amino acid sequence was studied using protein sequencer to analyze the unknown bands. Type I collagen derived from bone was used as a carrier whose properties and advantages were studied. The following conclusions are made from this study. 1. The limitation of molecluar weight in tbe early step of BMP purification procedure improves purification efficiency and stability, which makes the applicadon of BMP in big animalos possible. 2. In this method the purified BMP product also contains histone H3, histone 2B and other unknown proteins as well as BMP-2. 3. The atelocollagen derived from bone has proved to be an useful carrier for BMP. This carrier shows characteristics of self absorption and recalcification when embedded in tissues. 4. The minimun bone induction dose of BMP at each step (G-Ext:500μg, Hep:30μg, S200:50μg) and the optimal bone induction dose(G-Ext:5.0mg, Hep:2.0mg, S200:0.3mg) are determined, which makes the applicalion of BMP in cellular biology possible.
著者
及川 栄二 鈴木 直人 大越 哲也 海谷 幸利 福岡 雅人 前野 正夫 大塚 吉兵衛
出版者
硬組織再生生物学会
雑誌
日本硬組織研究技術学会雑誌 (ISSN:13405179)
巻号頁・発行日
vol.3, no.2, pp.85-92, 1994-10-29
被引用文献数
12

An attempt was made to compare cell growth and three different phosphatase activities between human alveolar bone-derived (HAB) cells from young (13-22 years old) patients and those from older (55-73 years old) patients. HAB cells growing out of individual bone fragments were harvested separately, and subcultured up to the 3rd passage. The growth and phosphatase activities of HAB cells from each individual were detemined on days 8, 24 and 40 of culture after seeding. The enzymes determined were alkaline phosphatase (ALPase), acid phosphatase (ACPase) and tartrateresistant acid phosphatase (TRACPase). Morphologically, the HAB cells used in this study were spindle-shaped in spite of aging. The individual data obtained were averaged within each age group, and the means (SD) of the two groups were compared. Cell growth showed a logarithmic pattern from day 3 in both age groups. The HAB cells from the young age groupreached confluence on day 11 of culture, whereas those from the older age group did so on day 21. The replication capacity of HAB cells from the young group was greater than that of cells from the older group. ALPase activity in both groups increased from day 8 to reach a maximum on day 24 of culture, followed by a decrease on day 40. On day 24, HAB cells from the young group showed in approximately 2.2-fold higher ALPase activity than those from the older group. On the other hand, no significant differences were observed in ACPase and TRACPase activities between the two groups between days 8 and 40.