著者

Mohd G Sghaireen Ahed Alkhatib Rayan Alswilem Jin Toriya Akiko Mizohata Mohammed Alrowili Santosh Patil Naoto Osuga Mohammad Khursheed Alam

出版者

硬組織再生生物学会

雑誌

Journal of Hard Tissue Biology (ISSN:13417649)

巻号頁・発行日

vol.26, no.3, pp.293-296, 2017-07-01 (Released:2017-07-11)

参考文献数

28

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The objectives are to investigate the association between dental caries and H. pylori, the association between dental caries and smoking among Saudi students, and to investigate the association between smoking and H. pylori among students with dental caries. The present cross sectional study included 120 male students from Aljouf University. Participating students were clinically examined for dental caries. Urine samples were tested for cotinine and stool samples were tested for H. pylori. Both cotinine and H. pylori was tested using strips especially prepared based on antigen-antibody reaction (COT, Innovacon Inc., USA, Helicobacter antigen Quick, GA Inc, Germany). Statistical analysis was done using Chi-Square test. The mean age of study participants was 22.37±1.50 years. The mean of teeth with caries was 9.57±4.68. There were 93 (77.5%) students positive for H. pylori, and 100 (83.3%) positive for cotinine. There was a significant relationship between cotinine and caries (P=0.000), and H. pylori and dental caries (P=0.000). The co-existence of H. pylori and cotinine was also significant among cases with caries (P=0.000). The present study showed that cotinine and H. pylori were significantly associated with dental caries. The co-existence of both among cases with dental caries may be a new finding and may work synergistically with each other.

著者

Hiroya Gotouda Ikuo Nasu Tetsuro Kono Yukari Ootani Takeshi Kanno Ryo Tamamura Takao Kuwada-Kusunose Kunihiro Suzuki Tomohiko Hirayama Tatsuya Hirayama Toshiro Sakae Hiroyuki Okada

出版者

硬組織再生生物学会

雑誌

Journal of Hard Tissue Biology (ISSN:13417649)

巻号頁・発行日

vol.26, no.1, pp.81-86, 2017 (Released:2017-01-06)

参考文献数

20

被引用文献数

5

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The aim of this study was to show the reaction varieties of human tooth enamel and dentin with a carbonated soft drink, Sprite. After one week exposing into the soft drink, the sliced tooth specimens were examined by macroscopic and microscopic and then carried out the micro X-ray diffraction analysis using a 100 μm diameter X-ray beam. The macroscopic and microscopic results showed the wide varieties from the one showing a very slight increase white-spot areas in the enamel to the another showing almost complete decay of the enamel. The crystallites in the examined enamel and dentin changed drastically as follows; the one enamel sample showed the decrease of crystal amount and some ionic substitutions, and the another dentin sample showed the increase in crystallinity and ionic substitutions. These results clearly showed that the original tooth enamel and dentin had their own crystallographic properties differing from each others. This study provided a crystallographic basic data which will be applied to a tayler-made individual preventive dentistry in near future.

著者

Ling Zheng Lin Wang Jie Qin Xiaolin Sun Tingting Yang Yuxin Ni Yanmin Zhou

出版者

硬組織再生生物学会

雑誌

Journal of Hard Tissue Biology (ISSN:13417649)

巻号頁・発行日

vol.24, no.1, pp.54-60, 2015 (Released:2015-01-20)

参考文献数

20

被引用文献数

1 10

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The temperature-sensitive triblock copolymer poly-(D, L-lactic acid-co-glycolic acid) (PLGA)-polyethylene glycol (PEG)-PLGA (PLGA-PEG-PLGA) is an FDA-approved material that has the ability to provide a sustained release of drugs and/or proteins. Platelet-rich fibrin(PRF)is second generation platelet concentration that contains growth factors such as transforming growth factor-β1 (TGF-β1), platelet derived growth factor-AB (PDGF-AB), and insulin-like growth factor-I (IGF-I). These growth factors affect the migration and proliferation of diverse cell types, including endothelial cells, smooth muscle cells, and osteoblast-like cells. This study sought to combine the hydrogel into scaffolds in order to serve as a sustained release system for PRF-derived growth factors. Poly (lactic-co-glycolic) acid (PLGA) and nano-hydroxyapatite (nHA) were used to prepare the hydrogel-containing scaffolds with the PRF-derived growth factors. We then investigated the effects of the hydrogel on modulating the activity of osteoblasts in vitro. We indicated that the hydrogel (Gel) was well-distributed in the inner surface of scaffolds, which themselves exhibited relatively interconnected pores with uniform sizes. The addition of the hydrogel didn’t affect their inherently high porosity. In vitro release tests indicated that the system containing nHA/PLGA/Gel/PRF provided for a slow and sustained release of PRF-derived growth factors. The results from our in vitro studies indicated that the MG63 cells cultured with both scaffold media extracts did not appear to have cytotoxic responses, and the nHA/PLGA/Gel/PRF system could improve the adhesion and proliferation of MG63 cells when compared to controls (p < 0.05). This in vitro evaluation suggests that the hydrogel-scaffold system is suitable as a model for bone tissue engineering, and that it allows for the sustained release of growth factors to improve bone reconstruction.

著者

Anuudari Erkhembaatar Ryoko Kawai Seeta Kato Waka Yoshida Kei Kamiya Yoshitaka Nagaya Tomofumi Hattori Yoshihiko Sugita Katsutoshi Kubo Hatsuhiko Maeda

出版者

硬組織再生生物学会

雑誌

Journal of Hard Tissue Biology (ISSN:13417649)

巻号頁・発行日

vol.23, no.2, pp.149-154, 2014 (Released:2014-04-14)

参考文献数

28

被引用文献数

1 1

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To date, more than 120 total types of HPV have been identified, and in a recent meta-analysis, HPV was confirmed as an independent risk factor for oral carcinoma. Some investigators have reported that HPV infection is related to certain factors, including the gender, age, alcohol consumption, smoking habit, sexual behavior and denture wearing. Denture wearing can lead to denture epulis, which is a hyperplasia of fibrous connective tissue caused by denture irritation. Recently, HPV infection was detected in the hyperplastic epithelium of denture fibroma, but it still has received little study. The objective of the present investigation, therefore, was to clarify the relationship between the hyperplastic epithelium of the denture epulis and HPV infection. DNA of 118 formalin-fixed, paraffin-embedded hyperplastic epithelium biopsies of epulis specimens was extracted. Firstly, HPV infection was detected by PCR using consensus primers. Secondary, PCR using HPV type-specific primers (low risk types 6 and 11; high risk types 16, 18 and 33) was done in positive PCR samples. HPV infection was also detected by in situ hybridization and immunohistochemical techniques. Eighteen (23.1 %) of the 78 cases of denture epulis were positive, and 2 (5.0 %) of the 40 cases of non-denture epulis were positive. The difference was statistically significant (p<0.05, using Fisher’s exact test). The most frequent type of HPV found in the 14 positive samples was HPV 16. It seems that the hyperplastic epithelium of denture epulis is easily infected with viruses because the epithelium is exposed daily to traumatic irritation from dentures. These results suggest that the hyperplastic epithelium of denture epulis might be an important reservoir for HPV infection of the oral region where later HPV-associated diseases, such as oral cancer and other oral lesions, may develop.

著者

及川 栄二 鈴木 直人 大越 哲也 海谷 幸利 福岡 雅人 前野 正夫 大塚 吉兵衛

出版者

硬組織再生生物学会

雑誌

日本硬組織研究技術学会雑誌 (ISSN:13405179)

巻号頁・発行日

vol.3, no.2, pp.85-92, 1994-10-29

被引用文献数

12

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An attempt was made to compare cell growth and three different phosphatase activities between human alveolar bone-derived (HAB) cells from young (13-22 years old) patients and those from older (55-73 years old) patients. HAB cells growing out of individual bone fragments were harvested separately, and subcultured up to the 3rd passage. The growth and phosphatase activities of HAB cells from each individual were detemined on days 8, 24 and 40 of culture after seeding. The enzymes determined were alkaline phosphatase (ALPase), acid phosphatase (ACPase) and tartrateresistant acid phosphatase (TRACPase). Morphologically, the HAB cells used in this study were spindle-shaped in spite of aging. The individual data obtained were averaged within each age group, and the means (SD) of the two groups were compared. Cell growth showed a logarithmic pattern from day 3 in both age groups. The HAB cells from the young age groupreached confluence on day 11 of culture, whereas those from the older age group did so on day 21. The replication capacity of HAB cells from the young group was greater than that of cells from the older group. ALPase activity in both groups increased from day 8 to reach a maximum on day 24 of culture, followed by a decrease on day 40. On day 24, HAB cells from the young group showed in approximately 2.2-fold higher ALPase activity than those from the older group. On the other hand, no significant differences were observed in ACPase and TRACPase activities between the two groups between days 8 and 40.