著者

谷田沢 道彦 東野 正三

出版者

一般社団法人日本土壌肥料学会

雑誌

日本土壌肥料學雜誌 (ISSN:00290610)

巻号頁・発行日

vol.24, no.4, pp.193-196, 1953-12-20

被引用文献数

1

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In the preceeding paper, the authors have found the rapid incorporation of inorganic phosphate absorbed through lear epidermis into some organic combinations including acid soluble-barium soluble phosphorus compounds primarily. In the present paper, resting on the basis of fine separation of phosphorus compounds in the use of paper-chromatography, the discussion on the forms of transferring phosphate after the foliar absorption are made. Thus one of the leaves of shakshina (Brassica chinensis L.) grow in field, about 30cm high, was applied with ca 1.5ml. of 1/75 M potassium dihydrogen phosphate containing P-32 in the strengh of 5 micro curie/ml. on a fine day. After 4 hours of this application, the poetiole was cut, and the exudation from this cut end was used for paper-chromatography as it was recommended by R. S. BANDURSKI and B. AXELROD. The developed paper strip was examined for its phosphorus spots by spraying HANES-ISHERWOOD reagent and its radio-activity by means of Geiger-Muller counter as it was reported by R. M. TOMARELI and K. FLOREY. Obtained paper-chramatograms and radiograms are shown in Fig. 1 and 2. Compared with Rf value of each particular known phosphorus compounds that had been determined in the same condition as that employed in the experimental procedure, transferring chief phosphorus compounds which have been newly synthesized in leaves are estimated to be confined to a few other compounds than any of ortho-phosphoric acid, glucose-1-phosphate, and fructose-6-phosphate. Under the condition which have been made, the sum of radio ortho-phosphoric acid, glucose-1-phosphate, and fructose-6-phosphate in the exudate is concieved less than 10% of total radio phosphorus in the exudate. S.ARONOFF has shown that the primary products of phosphate assimilation by soybean roots is fructose-1,6-diphosphate together with some phosphorylated organic acids, and also in our experiment, inorganic phosphate absorbed through leaf-surface is illustrated to be easily assimilated in leaf tissue and converted to such products.