著者

谷田沢 道彦 石原 隆

出版者

一般社団法人日本土壌肥料学会

雑誌

日本土壌肥料学会講演要旨集 (ISSN:02885840)

巻号頁・発行日

no.1, pp.82-83, 1955-04-01

著者

谷田沢 道彦 石原 隆

出版者

公益社団法人 日本農芸化学会

雑誌

日本農芸化学会誌 (ISSN:00021407)

巻号頁・発行日

vol.29, no.3, pp.229-234, 1955

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In the preceeding papers⁽³⁾the authors reported the radioactive contamination of plants⁽³⁾ in Japan covered with rain-out from H-bomb detonated in Spring 1954, at Bikini-atoll. In this paper; absorption of the fission products by sweet-clover and rice plants are released.<br> <b>I. Investigation on the radioactiv contamination of sweet-clover</b>.: Sample plants were taken from grass-land of Shiga Agr. College at three different period of time. In the field, sweet-clovers were cut down at about three weeks interval, and every time new leaves sprouted before long. Since early of June, radioactive density of rain had greatly decreased.<br> Plants ash was analysed in routine procedure of inorgenic separation⁽⁶⁾. Ratio of radioactivity of each analytical group was shown in Table. 1. By examining the table, it may be concluded that radioactive alkaline earths especially Sr₈₉ and S_??_⁹⁰ were selectively accumulated in plants from the fact that the increasing ratio of alkaine earths highly exceeded what was brouhht from different half-life time of each radioactive elements.<br> <b>II. Selective absorption of Bikini-ash by rice plants</b>.: A part of long-line (rope) on board No. 5 Fukuryu-Maru was ashed and then dissolved in nitric acid. Excess of acid were neutralized with ammonia to pH 5.2, and diluted with 1 distilled water. Resulted solution was evaluated as about 4m&mu;c/m<i>l</i> in its radioactivity.<br> Nbn-contaminated rice plants were cultivated in this radioactive solution for 20 days in greenhouse, then the absorption by plant of Bikini-ash was examined radiochemically. Group separations of radioactive elements in plant ashes and remaining culture solution were made chromatographically in the use of cation exchanger Amberlite IR-120. Elution curves were shown in Fig. 1 to 3. Total radioactivity and ratio of each separation group was shown in Table 2. From these figures and table, it becomes clear that rice plants accumulated larger parts of fission products in their roots, and selectively absorbed and translocated radioctive alkaline earths in their shoots even if the absorption ratio of Bikini fission products was comparatively small.

著者

谷田沢 道彦 東野 正三

出版者

一般社団法人日本土壌肥料学会

雑誌

日本土壌肥料學雜誌 (ISSN:00290610)

巻号頁・発行日

vol.24, no.4, pp.193-196, 1953-12-20

被引用文献数

1

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In the preceeding paper, the authors have found the rapid incorporation of inorganic phosphate absorbed through lear epidermis into some organic combinations including acid soluble-barium soluble phosphorus compounds primarily. In the present paper, resting on the basis of fine separation of phosphorus compounds in the use of paper-chromatography, the discussion on the forms of transferring phosphate after the foliar absorption are made. Thus one of the leaves of shakshina (Brassica chinensis L.) grow in field, about 30cm high, was applied with ca 1.5ml. of 1/75 M potassium dihydrogen phosphate containing P-32 in the strengh of 5 micro curie/ml. on a fine day. After 4 hours of this application, the poetiole was cut, and the exudation from this cut end was used for paper-chromatography as it was recommended by R. S. BANDURSKI and B. AXELROD. The developed paper strip was examined for its phosphorus spots by spraying HANES-ISHERWOOD reagent and its radio-activity by means of Geiger-Muller counter as it was reported by R. M. TOMARELI and K. FLOREY. Obtained paper-chramatograms and radiograms are shown in Fig. 1 and 2. Compared with Rf value of each particular known phosphorus compounds that had been determined in the same condition as that employed in the experimental procedure, transferring chief phosphorus compounds which have been newly synthesized in leaves are estimated to be confined to a few other compounds than any of ortho-phosphoric acid, glucose-1-phosphate, and fructose-6-phosphate. Under the condition which have been made, the sum of radio ortho-phosphoric acid, glucose-1-phosphate, and fructose-6-phosphate in the exudate is concieved less than 10% of total radio phosphorus in the exudate. S.ARONOFF has shown that the primary products of phosphate assimilation by soybean roots is fructose-1,6-diphosphate together with some phosphorylated organic acids, and also in our experiment, inorganic phosphate absorbed through leaf-surface is illustrated to be easily assimilated in leaf tissue and converted to such products.