著者

増田 義徳 高橋 秀二 村松 順信 小宮山 信雄 鶴田 亟次

出版者

公益社団法人日本産業衛生学会

雑誌

産業医学 (ISSN:00471879)

巻号頁・発行日

vol.1, no.5, pp.451-457, 1959-09

---

A method for estimating CS_2 in the biological materials was developed by modifying the existing procedure. The present procedure involves aeration of volatile CS_2 from biological materials and absorption of CS_2 in diethylamine-copper reagent. Conditions of aeration, such as temperature, pH of materials, air pressure in the aerator and flow rate through it were studied, and the concentration of CS_2 in different organs and body fluids of rabbits exposed to CS_2 was determined by the method with the following results. 1) On the condition that aeration time was CS_2 in body fluids or organs was highest in the blood, especially in blood corpuscles. Levels of CS_2 in the liver, the kidney, and the brain were nearly equal. In 25 hours after exposure to CS_2, free CS_2 was not over sixty minutes, a satisfactory recovery of CS_2 from materials was obtained, irrespective of temperature, pH of materials or air pressure in the aerator. 2) In the course of aeration for thirty minutes, most of free CS_2 of materials was recovered. Ninety minutes was found satisfactory to recover free CS_2 completely. 3) The recovery ratio for free CS_2 from biological materials was between 95 and 105 per cent. 4) Immediately after exposure to CS_2 at the concentration of 500 ppm, the amount of demonstrated in the body fluids or organs. 5) Concentration of CS_2 in the cerebrospinal fluid was relatively low, but it was almost of the same concentration as that in the serum.