著者

須釜 聡 立野 勝彦 灰田 信英

出版者

日本理学療法士協会

雑誌

理学療法学 = The Journal of Japanese Physical Therapy Association (ISSN:02893770)

巻号頁・発行日

vol.23, no.2, pp.72-79, 1996-03-31

参考文献数

14

被引用文献数

13

---

金沢大学大学院医学系研究科機能障害学関節拘縮発生にはコラーゲン中の架橋結合が関与し,この架橋結合はコラーゲンの可溶性に影響を及ぼす。今回我々は,ラットの足関節を7週間固定し筋および腱組織の可溶性の変化を検索しコラーゲン線維内の架橋結合の変化を推察した。その結果,ヒラメ筋については塩可溶コラーゲンとペプシン可溶化率に有意な減少を認めたが,アキレス腱については塩可溶コラーゲンに有意な減少を認めたのみであった。以上の結果から,7週間の固定により,コラーゲン線維内の架橋結合について,筋組織では架橋結合の数や強度の増加が推察されるが,腱組織では架橋結合の変化も少ないものと推察される。このことから,固定による影響は筋組織コラーゲンの方が大きいことが示唆され,組織の柔軟性の低下へつながる方向へ移行しているものと考えられる。 The purpose of this study was to investigate the change in solubility of 7 weeks immobilized Soleus muscle and Achilles tendon collagen. Left hind limb of six rats were immobilized for 7 weeks. Hydroxyproline was determined for the estimation of the collagen content in neutral salt soluble, acid soluble and insoluble collagen. In addition, insoluble collagen was digested with pepsin to determine solubility of insoluble collagen. In the case of Soleus muscle, collagen content to represent as a percent of wet weight was increased significantly during 7-week immobilization. In the case of Achilles tendon, collagen content to represent as a percent of wet weight did not change during the immobilization. In the case of Soleus muscle, the percentage of the salt solbule collagen to the total collagen was decreased significantly during 7-week immobilization. The percentage of the acid soluble and insoluble collagen to the total collagen did not change significantly during the immobilization. In the insoluble collagen, a rate of solubility with pepsin was decreased significantly. In the case of Achilles tendon, the percentage of the salt soluble collagen to the total collagen was decreased significantly during 7-weerk immobilization. The percentage of the acid soluble and insoluble collagen to the total collagen did not change significantly during the immobilization. In the insoluble collagen, a rate of solubility with pepsin did not change significantly. These results suggest that: 1) Solubility of Soleus muscle collagen, affected by intra and intermolecular crosslinks, changes during 7-week immobilization. 2) Solubility of Achilles tendon collagen, affected by intra and intermolecular cross-links, does not change remarkably during 7-week immobilization.