著者

草桶 秀夫 酒井 宏晃

出版者

福井工業大学

雑誌

福井工業大学研究紀要. 第一部 (ISSN:02868571)

巻号頁・発行日

vol.35, pp.191-196, 2005-03-18

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Luciferase gene (Luc) was introdeced into plants by using praticle gun methods and expression of the luciferase gene in the trasgenic Tobacco cells and leaves was studied by bonitoring lumineascence agter feeding the dsubstarate, luciferin. Luminescence in the cells and leaves transformed with pBE2113-luc vector, which contains a duplicated enhancer of caulifolwer mosaic virus 35s high expression promoter, was markedly stronger than that with pBI-luc and pMLH2113-Luc. The luminescence in the ransgenic Begonia leaves transhormed with pBE2113-luc vector using gold particle of 1.0 μm in deametor was higher than that using gold particle of 0.6 μm in deametor.