We frequently produce mispronunciation and hesitation in phonation. This paper analyzes the Japanese tongue twister in order to emphasize the effects of mispronunciation and hesitation. First, a statistical analysis of tongue twisters in phonation was carried out by employing phonemes, moras, and the place and manner of articulation. Next, the phonemes that occurred in mispronunciation and hesitation were surveyed by questioning to ten students. It was concluded that most hesitation and mispronunciation were made by articulating back vowels and front consonants. The results will assist in selecting words and sentences capable of speaking more fluently, as well as establishing speech synthesis by rule.