著者
IZAWA MASAO
出版者
The Japan Endocrine Society
雑誌
Endocrinologia Japonica (ISSN:00137219)
巻号頁・発行日
vol.37, no.2, pp.233-238, 1990

When the <I>in vitro</I> translation products of mRNAs from castrated animals (48h) were compared with those from androgen-treated animals (48 h) to survey the molecular mechanism of androgen-responsive gene expressions in the rat seminal vesicles, some peptide bands which were repressed in response to androgen were observed. From these findings, we constructed a partial cDNA library of poly (A+) RNAs which had been isolated from the seminal vesicles of castrated rats (48 h) and modestly enriched with respect to the concentration of androgen-repressed mRNAs by sucrose density gradient centrifugation, and screened by differential colony hybridization. One cDNA clone, pSvr-1, whose mRNA is markedly induced within 24h after castration of the animal in the seminal vesicles as well as in the ventral prostate, was isolated. pSvr-1 hybridized to a mRNA of 1, 700 nucleotides in length. Partial sequence analysis showed that this clone had highly homologous but not identical sequences to those reported for rat sulfated glycoprotein-2. This cDNA clone may provide a useful probe for the study of the negative regulation mechanism of gene expression by androgens.

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こんな論文どうですか? cDNA Cloning of Androgen-Repressed mRNA in Rat Seminal Vesicles: Partial Charact(IZAWA MASAO),1990 … https://t.co/LwOuaPHPLn
こんな論文どうですか? cDNA Cloning of Androgen-Repressed mRNA in Rat Seminal Vesicles: Partial Charact(IZAWA MASAO),1990 … https://t.co/LwOuaPHPLn
こんな論文どうですか? cDNA Cloning of Androgen-Repressed mRNA in Rat Seminal Vesicles: Partial Charact(IZAWA MASAO),1990 … https://t.co/LwOuaPHPLn
こんな論文どうですか? cDNA Cloning of Androgen-Repressed mRNA in Rat Seminal Vesicles: Partial Charact(IZAWA MASAO),1990 … https://t.co/LwOuaPHPLn
こんな論文どうですか? cDNA Cloning of Androgen-Repressed mRNA in Rat Seminal Vesicles: Partial Charact(IZAWA MASAO),1990 … https://t.co/LwOuaPZr9X
1 1 https://t.co/RahYK6gaPv
こんな論文どうですか? cDNA Cloning of Androgen-Repressed mRNA in Rat Seminal Vesicles: Partial Charact(IZAWA MASAO),1990 … https://t.co/LwOuaPHPLn

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