著者

IZAWA MASAO

出版者

The Japan Endocrine Society

雑誌

Endocrinologia Japonica (ISSN:00137219)

巻号頁・発行日

vol.37, no.2, pp.233-238, 1990

被引用文献数

2

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When the <I>in vitro</I> translation products of mRNAs from castrated animals (48h) were compared with those from androgen-treated animals (48 h) to survey the molecular mechanism of androgen-responsive gene expressions in the rat seminal vesicles, some peptide bands which were repressed in response to androgen were observed. From these findings, we constructed a partial cDNA library of poly (A+) RNAs which had been isolated from the seminal vesicles of castrated rats (48 h) and modestly enriched with respect to the concentration of androgen-repressed mRNAs by sucrose density gradient centrifugation, and screened by differential colony hybridization. One cDNA clone, pSvr-1, whose mRNA is markedly induced within 24h after castration of the animal in the seminal vesicles as well as in the ventral prostate, was isolated. pSvr-1 hybridized to a mRNA of 1, 700 nucleotides in length. Partial sequence analysis showed that this clone had highly homologous but not identical sequences to those reported for rat sulfated glycoprotein-2. This cDNA clone may provide a useful probe for the study of the negative regulation mechanism of gene expression by androgens.

著者

IZAWA MASAO

出版者

The Japan Endocrine Society

雑誌

Endocrinologia Japonica (ISSN:00137219)

巻号頁・発行日

vol.38, no.1, pp.61-66, 1991

被引用文献数

4

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To further survey the molecular mechanisms underlying the involution of steroid hormone-dependent rat tissues, we undertook experiments to test whether or not any significant correlation between the tissue involution and expressions of rat sulfated glycoprotein 2 (SGP-2) and pSvr-1 genes, which had been initially cloned from the Sertoli cells and the seminal vesicles, respectively, and then identified as androgen repressed messages both in the ventral prostate and in the seminal vesicles, could be observed in steroid hormone-dependent rat tissues. Expressions of these genes were stimulated within 48 h after castration of animals both in the ventral prostate and in the seminal vesicles as reported previously, but not significantly altered by ovariectomy in the uterus. Expressions of these genes in the thymus were significantly repressed by the administration of dexamethasone and/or cycloheximide. Although the roles of expressions of SGP-2 and pSvr-1 genes in steroid hormonedependent tissues remain unclear, their presence might become useful molecular markers of tissue involution not only in androgen-dependent rat tissues but also in glucocorticoid-dependent ones, and also provide excellent model systems for the study of negative regulation mechanism of gene expression by steroid hormones.