著者

小林 豊一 原田 ひとみ 柴田 健介 廣瀬 俊一

出版者

The Japanese Society of Inflammation and Regeneration

雑誌

炎症 (ISSN:03894290)

巻号頁・発行日

vol.13, no.4, pp.383-386, 1993

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The effect of several agents on mitogen-induced proliferative response<I>in vitro</I>was studied by using colorimetric MTT [3- (4, 5-dimenthyl-2-thiazolyl) -2, 5-diphenyl-2H tetrazolium bromide] assay. Immuno-modulator levamisole and TOK-8801 enhanced concanavalin A and lipopolysaccharide response in murine splenocytes at concentrations of 10<SUP>-6</SUP>-10<SUP>-5</SUP>M and 10<SUP>-4</SUP>M, respectively. Immunosuppressant mizoribine decreased dose dependently this response.<BR>These results show that MTT assay may be a useful method for the evaluation of immunological affecting agents on cell proliferative response.