- 著者
-
小林 豊一
原田 ひとみ
柴田 健介
廣瀬 俊一
- 出版者
- The Japanese Society of Inflammation and Regeneration
- 雑誌
- 炎症 (ISSN:03894290)
- 巻号頁・発行日
- vol.13, no.4, pp.383-386, 1993
The effect of several agents on mitogen-induced proliferative response<I>in vitro</I>was studied by using colorimetric MTT [3- (4, 5-dimenthyl-2-thiazolyl) -2, 5-diphenyl-2H tetrazolium bromide] assay. Immuno-modulator levamisole and TOK-8801 enhanced concanavalin A and lipopolysaccharide response in murine splenocytes at concentrations of 10<SUP>-6</SUP>-10<SUP>-5</SUP>M and 10<SUP>-4</SUP>M, respectively. Immunosuppressant mizoribine decreased dose dependently this response.<BR>These results show that MTT assay may be a useful method for the evaluation of immunological affecting agents on cell proliferative response.