著者

花海 清 熊谷 勝男

出版者

日本炎症・再生医学会

雑誌

炎症 (ISSN:03894290)

巻号頁・発行日

vol.7, no.3, pp.253-258, 1987-05-01 (Released:2010-04-12)

参考文献数

23

---

Carboxyethylgermanium sesquioxide, Ge-132 was investigated for its effect on the expression of HLA-DR ant·pens or IgG-Fc receptors (FcRl) on human peripheral blood monocytes and a human myelogenous leukemia cell line U937 in culture. Ge-132, when added in the cultures of either purified peripheral monocytes or U937 cells, did not produce any significant effect on expression of either HLA-DR antigens or FcRl on the cell. The peripheral blood mononuclear cells (PBMC) when being incubated with Ge-132 (800μg/ml), however, produced a factor inducing augmentation of HLA-DR and FcR expression on the monocytes or U937. Pretreatment of this PBMC culture fluid with pH2.0 or specific antiserum against human interferon γ (IFNγ) resulted in a marked reduction of both HLA-DR and FcR-inducing activities, suggesting that a major factor induced by Ge-132 in the PBMC may be IFNγ. These results indicate that Ge-132 stimulates PBMC to produce a soluble factor (IFNγ), which in turn augments the expression of HLA-DR antigens and FcRl on the cell of myelogenous or macrophage series.

著者

深澤 俊行 菊地 誠志 濱田 毅 田代 邦雄

出版者

日本炎症・再生医学会

雑誌

炎症 (ISSN:03894290)

巻号頁・発行日

vol.20, no.3, pp.209-213, 2000-05-29 (Released:2010-04-12)

参考文献数

18

---

The clinical features of MS in Japanese are said to be characterized by high incidence of optico-spinal MS (OS-MS), although the frequency of it has recently decreased. The term OS-MS is used to describe a subgroup of patients diagnosed with MS, in which the clinically determined lesions are confined to the optic nerves and the spinal cord. The OS-MS is characterized by a female preponderance, a later onset, a higher score of the expanded disability status scale of Kurtzke (EDSS), milder abnormalities on brain MRIs, higher sero-positive rates for some autoantibodies, and higher cell counts and protein levels in the cerebrospinal fluid, compared with conventional MS. The OS-MS is positively associated with HLA-DPB1*0501 allele, and the HLA prohile are different between OS-MS and conventional MS. Therefore, the OS-MS is immunogenetically as well as clinically a distinct subtype of MS.

著者

吉良 潤一

出版者

日本炎症・再生医学会

雑誌

炎症 (ISSN:03894290)

巻号頁・発行日

vol.20, no.2, pp.137-144, 2000-03-29 (Released:2010-04-12)

参考文献数

8

被引用文献数

2 1

---

We recently reported the occurrence of myelitis in adults with atopic dermatitis (AD) . In order to clarify the clinical features of myelitis with atopy, we retrospectively studied 68 consecutive patients with acute or subacute myelitis diagnosed in Kyushu University Hospital during the past 20 years. Of 40 patients with myelitis seen between 1994 and 1998, 19 (48%) had either AD or bronchial asthma (BA), while 2 of 28 (7%) myelitic patients treated between 1979 and 1993 did. Of the 40 patients with myelitis diagnosed between 1994 and 1998, 29 (73%) had hyperlgEaemia and 32 (80%) had mite antigen-specific IgE, while of 82 healthy controls and 43 patients with neurodegenerative disorders, 21% and 23% had hyperlgEaemia and 40% and 32% had mite antigen-specific IgE respectively. Seventeen myelitic patients with AD showed an involvement of primarily the posterior column of the spinal cord and paresthesia/dysesthesia in all four limbs, whereas 3 patients with BA, including 2 adults, showed most common involvement of the anterior horn cells with muscle weakness and atrophy following acute asthmatic attacks. In addition, 12 myelitic patients who had hyperlgEaemia and IgE antibodies to mite antigens but neither AD nor BA also showed partial involvement of the spinal cord. Among these myelitis patients with atopy, pleocytosis in the cerebrospinal fluid was rare, peripheral blood eosinophilia was frequent and corticosteroids were less beneficial. These findings suggest an emergence of myelitis associated with atopy in Japan.

著者

藤井 恵美子 村木 篁

出版者

日本炎症・再生医学会

雑誌

炎症 (ISSN:03894290)

巻号頁・発行日

vol.15, no.2, pp.155-160, 1995-03-31 (Released:2010-04-12)

参考文献数

17

被引用文献数

1 1

---

We examined the changes in vascular permeability induced by inflammatory mediators in mouse skin by the Pontamine sky blue (PSB) leakage technique. Five min after i.v. administration of PSB, mediators or saline were injected (0.1 ml/site, s.c.) into the back. Sixty min later, the dye accumulated in the skin was determined colorimetrically. Plateletactivating factor (PAF, 45-180 pmol/site), 5-hydroxy-tryptamine (5-HT, 120-960 pmol/site), substance P (100-1, 000 pmol/site) and histamine (550-5, 500 pmol/site) produced dose-related increases in vascular permeability. The dose response curve induced by bradykinin (20-80 nmol/site) was bellshaped. Pretreatment with indomethacin significantly reduced the increase in vascular permeability elicited by the 5 substances tested. Coadministration of prostaglandin (PG) E2 partially reversed the inhibitory effect of indomethacin on the PAF- or histamine induced-increase in vascular permeability. Increases in vascular permeability induced by 5-HT, substance P and bradykinin were inhibited by NG-nitro-l-arginine methyl ester (L-NAME) and methylene blue, but not by D-NAME ; however, PAF-and histamine-induced increases in vascular permeability were not affected by L-NAME.These results suggest that eicosanoids may play a role in the effect of all 5 substances tested, and especially the increases in plasma extravasation induced by PAF and histamine are mediated by the production of PGE2, and that NO may play a role, at least partly, in the case of 5-HT, substance P and bradykinin.

著者

中川 武正

出版者

日本炎症・再生医学会

雑誌

炎症 (ISSN:03894290)

巻号頁・発行日

vol.4, no.3, pp.201-207, 1984-07-01 (Released:2010-04-12)

参考文献数

30

---

The role of IgG4 antibodies in immediate hypersensitivity will be discussed in this paper, both from experimental and clinical point of view. The data described will show that 1) not only IgE but also IgG4 molecules are present on human basophils, 2) IgE-mediated basophil degranulation may be inhibited by passive sensitization with excess amounts of IgG4 antibodies, 3) allergen-specific IgG4 antibodies become prominent upon repeated parenteral stimulation with antigen, such as immunotherapy and bee-stings, in asthmatics and in bee keepers. The overall results suggest that IgG4 antibodies may react as blocking antibodies which produce protective effect during clinical course, in a certain type of allergic disorders. The role of IgG4 antibodies as anaphylactic antibodies will be also briefly discussed.

著者

河野 善行 宮地 良樹

出版者

日本炎症・再生医学会

雑誌

炎症 (ISSN:03894290)

巻号頁・発行日

vol.20, no.2, pp.119-129, 2000-03-29 (Released:2010-04-12)

参考文献数

27

---

Cosmetics and Quasi-drugs will play new important roles in the aging and stressful society. We have proposed 3 clinical approaches, i.e., anti drying, anti-UV radiation and antioxidation to prevent the skin disorders and aging. Especially, the importance of anti-oxidation is discussed in this paper. The results of the investigation of the peroxidation on the skin surface using a CL-HPLC system, it was considered that singlet oxygen was the key active oxygen species on a human skin. We have clarified the reaction rate constants of the skin surface lipids and thiotaurine with singlet oxygen. Thiotaurine is known as a compound in a metabolism of sulfur containing amino acids in a mammal. We propose that the reconstruction and the reinforcement of the anti-oxidation mechanism in the living system will be useful new clinical approaches in the skin.

著者

武元 則人 丸山 博文 川村 秀樹 小松 靖弘 油田 正樹 細谷 英吉

出版者

日本炎症・再生医学会

雑誌

炎症 (ISSN:03894290)

巻号頁・発行日

vol.9, no.2, pp.137-140, 1989-03-10 (Released:2010-04-12)

参考文献数

8

---

The mitogenic activity of TJ-48 in murine lymphoid cells was examined to get a clue for the augmentation of antibody production by TJ-48.Mitogenic activity of TJ-48 was detected in spleen cells, lymphonodus cells but not in thymocytes. The effect was abolished by pretreatment with anti-Ig antibody but not affected by pretreatment with anti-Thy 1.2 antibody. Coculture of spleen cells with TJ-48 resulted in increased number of sIgM-, sIgG- or sIgD-positive cells according to FAGS analysis. The increase in mitosis by TJ-48 was abolished by the elimination of adherent cells and the readdition of adherent cells recovered the effect of TJ-48. These data indicate that TJ-48 is a B cell mitogen and the activity is T cell-independent and adherent cell-dependent. The mitogenic activity of TJ-48 may account for the augmentation of the immune response.

著者

湯尾 明

出版者

日本炎症・再生医学会

雑誌

炎症 (ISSN:03894290)

巻号頁・発行日

vol.16, no.5, pp.307-318, 1996-09-10 (Released:2010-04-12)

参考文献数

26

---

New method for isolation of human monocytes without adhesion process by using centrifugal elutriator has been recently established, and the effects of inflammatory cytokines such as TNF and MCAF on suspended monocytes were investigated. Both cytokines did not induce or only minimally induced superoxide release in human monocytes, but potently primed monocytes for enhanced release of superoxide upon stimulation with subsequent agonists, and these two cytokines primed monocytes in an additive manner. TNF induced minimal effects on intracellular signaling pathways, whereas MCAF rapidly induced intracellular signalings such as pH changes in a similar manner with FMLP.To clarify interaction between monocytes and endothelial cells during inflammatory responses, double chamber in vitro migration assay system was established. Both monocytes and endothelium produced several inflammatory cytokines such as TNF, IL 1, MCAF, and GM-CSF into the apical but not basilar side of the endothelial cell monolayer during monocyte adhesion to and transmigration through endothelial cells. Thus, monocyte chemoattractant such as MCAF released into the apical side could inhibit monocyte transendothelial migration, suggesting possible in vivo selfregulatory system of inflammation.In regard to the signal transduction in human monocytes, tyrosine kinases and their substrates are of particular importance, and several signaling molecules such as MAP kinase, STAT 5, and vav product have been identified. Although the signaling pathways utilized by soluble inflammatory stimuli such as cytokines and chemotactic factors have been substantially clarified, those utilized by adhesive stimuli are still largely unknown particularly in human phagocytes, and awaits much further investigation.

著者

水島 裕

出版者

日本炎症・再生医学会

雑誌

炎症 (ISSN:03894290)

巻号頁・発行日

vol.15, no.2, pp.93-103, 1995-03-31 (Released:2010-12-10)

参考文献数

34

著者

正田 悦朗 石川 斉 広畑 和志 渡辺 信

出版者

日本炎症・再生医学会

雑誌

炎症 (ISSN:03894290)

巻号頁・発行日

vol.4, no.4, pp.535-538, 1984-11-01 (Released:2010-04-12)

参考文献数

7

著者

細田 〓弘

出版者

日本炎症・再生医学会

雑誌

炎症 (ISSN:03894290)

巻号頁・発行日

vol.20, no.5, pp.567-569, 2000-09-29 (Released:2010-04-12)

参考文献数

1

著者

鏑木 淳一 東條 毅 本間 光夫

出版者

日本炎症・再生医学会

雑誌

炎症 (ISSN:03894290)

巻号頁・発行日

vol.1, no.5, pp.607-613, 1981-11-01 (Released:2010-04-12)

参考文献数

32

---

Profiles of autoantibodies in patients with scleroderma were reviewed and their possible clinical significance was discussed in this paper.Many autoantibodies including antinuclear antibodies and rheumatoid factors are detected in patients with scleroderma, especially in those with hypergammaglobulinemia. Fluorescent antinuclear antibodies were found in 36-97% of the serum of patients with scleroderma. The specificities of these antinuclear antibodies have been actively investigated in various laboratories using various nuclear antigens and many specificities were recently identified. Among them, two new antibody systems; anti-Og (Scl-1/Scl-70) antibody and anti-centromere antibody, were found to be highly specific for this disorder. Anti-Og antibody was detected in 33% of scleroderma patients and the patients with this antibody tended to have relatively advanced dermal sclerosis and pulmonary fibrosis. Anti-centromere antibody was reported to be present in the serum of patients mainly with CREST syndrome. Other antinulcear antibody systems; e.g. anti-DNA, anti-RNP, and anti-SS·B, were also seen in patients with scleroderma. However, when the patients with overlap syndrome were excluded, anti-RNP antibody was major antibody system in scleroderma. In contrast to the patients with anti-Og antibody, those with antiRNP antibody tended to have much milder sclerodermatous skin change and better prognosis.The presence of the serum autoantibodies in scleroderma patients and the association of these antibodies to patient's clinical characteristics suggest that these antibodies may play some significant roles in developping clinical features and/or might be closely related to the pathogenesis of this disease. Therefore it seems to be important to investigate these antibodies in order to clarify the pathogenesis of scleroderma.

著者

吉川 敏一 古川 泰正 村上 正志 渡辺 幸市 杉野 成 近藤 元治

出版者

日本炎症・再生医学会

雑誌

炎症 (ISSN:03894290)

巻号頁・発行日

vol.4, no.4, pp.605-607, 1984-11-01 (Released:2010-04-12)

参考文献数

26

著者

吉川 敏一 吉田 憲正 近藤 元治

出版者

日本炎症・再生医学会

雑誌

炎症 (ISSN:03894290)

巻号頁・発行日

vol.13, no.5, pp.413-421, 1993-09-30 (Released:2010-04-12)

参考文献数

64

被引用文献数

1 1

---

Oxygen-derived free radicals are mainly produced by phagocytic cells and hypoxanthine-xanthine oxidase system at inflammatory site. These oxygen metabolites and lipid peroxidation have been implicated in cell damage, interactions with other inflammatory mediators and modulation of vascular response. It is very important to modulate free radical-induced inflammatory reaction in various organs by administration of radical scavengers and antioxidants.

著者

室田 誠逸

出版者

日本炎症・再生医学会

雑誌

炎症 (ISSN:03894290)

巻号頁・発行日

vol.20, no.2, pp.103-105, 2000-03-29 (Released:2010-04-12)

著者

近藤 元治

出版者

日本炎症・再生医学会

雑誌

炎症 (ISSN:03894290)

巻号頁・発行日

vol.20, no.2, pp.99-102, 2000-03-29 (Released:2010-04-12)

著者

三橋 秀基 梶山 浩 野島 美久

出版者

日本炎症・再生医学会

雑誌

炎症 (ISSN:03894290)

巻号頁・発行日

vol.19, no.3, pp.137-142, 1999-05-31 (Released:2010-04-12)

参考文献数

16

---

Sulfite is a major air pollutant which can cause inflammatory reactions in the respiratory tract characterized by an influx of neutrophils. In addition, sulfating agents are widely used in the food and beverages industries as antimicrobials or antioxidants. Sulfite is also an important intermediatory compound in the metabolic pathway from sulfur containing amino acids to sulfate in mammalians. Human serum contains some amounts of sulfite. But the production and physiological role of sulfite in mammalians are still unclear. We have recently shown that human neutro-phils produced significant amounts of sulfite in vitro in response to lipopolysaccharide (LPS) stimulation. In a rat model of sepsis induced by in vivo injection of LPS, sulfite concentration in serum was significantly increased. These results suggest that sulfite can be actively generated by mammalian cells in vivo and in vitro. In this brief review article, we discuss a potential role of sulfite as an endogenous mediator of inflammation.

著者

能勢 高志 鶴見 介登 川田 憲司 永井 博弌 山田 博章 薬王 郁久 尾島 昭次 江田 昭英

出版者

日本炎症・再生医学会

雑誌

炎症 (ISSN:03894290)

巻号頁・発行日

vol.10, no.6, pp.471-476, 1990

---

A glomerulonephritis was induced in mice by injection of subnephrotoxic dose of nephrotoxic serum (NTS) after preimmunization with rabbit IgG. In order to characterize this glomerulonephritis, light, electron and immunofluorescence microscopic studies were carried out 15 days after NTS injection, the time when increases in urinary protein and serum cholesterol and a decrease in serum albumin were apparent.<BR>Characteristic changes were widespread thickening of capillary walls and narrowing of the capillary lumen owing to widening of mesangial areas. In those capillary walls, the mesangial interposition into subendothelial areas was often noted ultrastructurally and double track was confirmed on sections stained with PAM. Linear deposition of mouse IgG was detected in capillary walls by immunofluorescence. In severely affected glomeruli, PAS-positive hyaline nodular lesion was observed light microscopically and massive mesangial deposits ultrastructurally.<BR>Visceral epithelial cells demonstrated fusion of the foot processes, microvilli formation, occasional proliferation and enlargement. Parietal epithelial cells proliferated, forming cellular or fibrocellular crescent.<BR>Based on these characteristics, it appears this nephritic model shares a common pathology with human membranoproliferative glomerulonephritis type 1 and crescentic glomerulonephritis and can be considered an appropriate model for producing severe nephritis for short periods.

著者

谷中 昭典

出版者

日本炎症・再生医学会

雑誌

炎症 (ISSN:03894290)

巻号頁・発行日

vol.19, no.3, pp.129-135, 1999-05-31 (Released:2010-04-12)

参考文献数

49

---

Nitric oxide (NO) has been shown to elicit both favorable and unfavourable effects on gastrointestinal (GI) system. NO is generated from L-arginine by two different types of NO synthase (NOS) . One type of NOS, constitutive NOS (cNOS), is con-stitutively expressed by endothelial cells (eNOS) and by neural cells (nNOS) . cNOS-derived NO causes a variety of physiogical effects by stimulating guanylate cyclase-cylic GMP system, effects genereally beneficial to GI system. For example, nNOS-derived NO modulates GI motility. eNOS-derived NO increases mucosal blood flow, and stimulates production of mucus and HCO3-, all of which enhance mucosal defense and repair system. Based on these background, several attemps have been made to use NO as a therapeutic drugs. For example, tetraprenylacetone (teprenone) has been shown to enhance gastric mucosal restitution by upregulating cNOS activity. NO-releasing NSAIDs have been developed to mitigate NSAIDs induced mucosal injury by protection afforded by NO. In contrast, other type of NOS, inducible NOS (iNOS), is transiently expressed in macrophages in response to stimuli, such as bacterial invasion. iNOS generates huge amount of NO, that pre-vents invasion of bacteria, but causes serious injury in host tissues. For example, Helicobacter pylori upregulates iNOS expression in gastric mucosa, and enhances release of NO, thereby accelerating epithelial cell apoptosis. In chronic inflammatory bowel diseases, inceased expression of mucosal iNOS is closely associated with developement of mucosal injury. However, it should be noted that in certain circum-stances iNOS affords mucosal protection against noxious stimuli, and promotes mucosal repair after injury.

著者

小田 隆治 鹿取 信

出版者

日本炎症・再生医学会

雑誌

炎症 (ISSN:03894290)

巻号頁・発行日

vol.11, no.1, pp.9-17, 1991-01-10 (Released:2010-04-12)

参考文献数

30

---

A series of the process of neutrophil extravasation induced by topical application of leukotriene B4 (LTB4) or formyl-methionyl-leucyl-phenylalanine (fMLP) on microvasculature of the hamster cheek pouch can be divided into five steps; I) rolling on the venular endothelium, II) adhesion on the endothelium, III) passage between the endothelial cells, IV) staying in the venular wall, V) migration from the venular wall to the interstitial space. In step II, topical application of chemoattractants on the microvasculature caused an increase in the number of neutrophils adhered to the venules. In step III, the size of the adhered neutrophils became gradually smaller and finallly disappear from the vascular lumen, as they were observed on the monitor screen. Over 80-90% of the adhered neutrophils passed through the endothelial cells. The whole process from step II to III took about 7 minutes. In step IV, the neutrophils which passed through the endothelial cells stay for about 30 minutes in the venular wall. In step V, neutrophils penetrated the basement membrane and migrated into the interstitial space.Step II and III were suppressed by a cyclic AMP phosphodiesterase inhibitor, fibronectin inhibitor and cytochalasin B. From these results, it was suggested that chemoattractant may cause the contraction of microfilament of neutrophil, and the contraction may induce the expression of adhesive glycoprotein on the neutrophil membrane. The increase of cyclic AMP in the neutrophil may inhibit the contraction of microfilament and/or the expression of adhesive glycoprotein of neutrophil. Dexamethasone did not affect the step I to IV, but suppressed the step V. Dexamethasone may inhibit synthesis and/or release of protease (s) in the neutrophil granules which degrade the basement membrane. In fact, the collagenase inhibitor suppressed the step V, although it did not the step I to IV. Thus, neutophil-derived collagenase may act on the passage of neutrophil through the basement membrane. The process of neutrophil extravasation is the chain of reaction.