- 著者
-
伊藤 貞嘉
阿部 圭志
尾股 健
保嶋 実
吉永 馨
- 出版者
- 社団法人 日本腎臓学会
- 雑誌
- 日本腎臓学会誌 (ISSN:03852385)
- 巻号頁・発行日
- vol.30, no.1, pp.85-90, 1988
To examine the role of prostaglandins (PGs) in the macula densa mechanism of renin release, rabbit afferent arteriole (Af) alone and afferent arteriole with macula densa attached (Af+MD) were microdissected and incubated consecutively. Hourly renin release rate from a single Af (or Af+MD) was calculated and expressed as ngAI·h<SUP>-1</SUP>·Af<SUP>-1</SUP> (or Af+MD<SUP>-1</SUP>)/h (where AI is angiotensin I). Basal renin release rate from Af was 0.84±0.14ngAI·h<SUP>-1</SUP>·Af<SUP>-1</SUP>/h (X±SEM, n=23) and remained stable throughout the incubations. Basal renin release rate from Af+MD was 0.33±0.04ngAI·h<SUP>-1</SUP>Af+MD<SUP>-1</SUP>/h (n=17), which was significantly lower (p<0.01) than that from Af. When furosemide (1.5 mM) was added to Af, no significant change in renin release rate was observed. However, when furosemide was added to Af+MD, renin release rate increased from 0.40±0.05 to 1.59±0.15ngAI·h<SUP>-1</SUP>·Af+MD<SUP>-1</SUP>/h (n=10, p<0.01). After the pretreatment with indomethacin, a cyclooxygenase inhibitor, furosemide still increased renin release rate from 0.17±0.02 to 0.56±0.09 ng AI·h<SUP>-1</SUP>·Af+MD<SUP>-1</SUP>/h (n=5, p<0.05) ; however, indomethacin pretreatment reduced both basal and furosemide-stimulated renin release rate (p<0.05). In the presence of PGI<SUB>2</SUB> (10 μM), renin release rate from Af increased from 0.45±0.14 to 1.49±0.53 ng AI·h<SUP>-1</SUP>·AN/h (n=9, p<0.05), and further increased to 4.50±1.24 ng AI·h<SUP>-1</SUP>·Af<SUP>-1</SUP>/h (p<0.02) after removal from PGI<SUB>2</SUB>. When PGE<SUB>2</SUB> (10 μM) was added to Af+MD, renin release rate increased from 0.54±0.09 to 1.26±0.24ng AI·h<SUP>-1</SUP>Af+MD<SUP>-1</SUP>/h (n=8, p< 0.05). However PGE<SUB>2</SUB> had no effect on renin release rate from Af alone. We concluded that (1) the prostaglandin system may be a modulating factor of response in the macula densa mechanism of renin release, (2) PGI<SUB>2</SUB> has direct action on renin release from affer-ent arteriole, and (3) PGE<SUB>2</SUB> may participate in the control of renin release through the action on the macula densa.