著者

西村 武 市川 陽三 千葉 忠二郎

出版者

Japanese Society of National Medical Services

雑誌

医療 (ISSN:00211699)

巻号頁・発行日

vol.11, no.8, pp.638-643, 1957

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The present paper deals with the characteristics of cold-agglutinin, found in a particular case of blood-group B. The case was noted at the time of cross-match test, when its serum showed pan-agglutination to all groups of tested red cells, including group B.<br>At room temperature, its serum agglutinated all types of human red cells tested. At 20°C, the serum agglutinated its own red cells and the reaction disappeared at 37°C. The reaction however, re-appeared when these sets of test-tubes were cooled down to 20°C. Thus the reversibllity of the reaction was apparent.<br>The colder the temperature, the higher the agglutinin titer. With group B and O cells, titers of 64 were obtained at 0°C and at 5°C, titers of 16, 8, 4 and 2 were obtained at 10°C, 15°C, 20°C and 25°C, respectively. At 37°C, no reaction occurred.<br>Once the agglutinins were consumed in the cold agglutination, the supernatant showed normal reactibility in cross-match tests. The absorbed agglutinins were eluted again from red cells with saline solution at high temperature.<br>The agglutinin titer was fairly stable. It did not decrease even after 30 days in an icebox. The agglutinin was resistant to the heating at 60°C for 30 days, but not at 65°C.<br>Normal specimens of group B tested for the control showed titers of cold-agglutinin raging from 1 to 16, and their average was 5. None of the control reacted at 20°C. The authors discussed the possibility of finding out such particular specimens, at the time of cross-match tests.