著者

石津 日出雄 山本 雄二 守屋 文夫 宮石 智 仙波 昌三

出版者

Okayama Medical Association

雑誌

岡山医学会雑誌 (ISSN:00301558)

巻号頁・発行日

vol.103, no.3, pp.145-153, 1991-06-18 (Released:2009-08-24)

参考文献数

31

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The progress of sex identification methods for forensic materials such as dried bloodstains and others in legal medicine is reviewed.Dixson and Torr (1956) first described a scientific method to identify human sex from forensic materials by detection of X chromatin in female cell nuclei. In 1972, we introduced a method for detecting Y chromatin in the human interphase nuclei by fluorescent microscopy as a new tool for decisive male sex determination from forensic materials. This method was accepted in forensic practice as the only reliable means to identify sex. In 1983, we reported sexes were distinguishable from dried bloodstains by the ratio of sex hormones, testosterone and progesterone, determined by radioimmunoassay. Gene analytical methods to prove the human Y chromosome specific DNA sequences have been introduced in this field. We also examined the method of Witt and Erickson (1989) to detect Y and X chromosome specific sequences using the polymerase chain reaction (PCR), and we improved this technique to exhibit its ability to identify sex in forensic practice. Our improved PCR method is expected to have a broad applicability in forensic practice because of its simplicity, sensitivity and reliability.