著者

村上 洋 瀬古 亜紀子 安積 真澄 上嶋 夏子 吉栖 肇 中野 博文 北畑 寿美雄

出版者

日本応用糖質科学会

雑誌

Journal of Applied Glycoscience (ISSN:13447882)

巻号頁・発行日

vol.50, no.2, pp.117-120, 2003

被引用文献数

4 36

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ラクトビオン酸(LA)はラクトースから合成されるアルドビオン酸で,ビフィズス菌増殖活性をもち,溶解性の高いミネラル補給剤として利用可能であるなど,用途に富んだ糖質素材である.しかし,現在までは,実用に耐える大量生産方法がなく,日本では化学合成法により試薬として少量市販されているのみであった.筆者らは,ラクトビオン酸の安価な大量生産方法の開発をめざし,Burkholderia cepaciaの1菌株を用いた発酵生産条件を検討した.その結果,回分培養では,4日間の培養により,2009/Lのラクトビオン酸が生成した.また乳糖と炭酸カルシウムの逐次添加を行う流加培養では,10日間の培養により,培養液中に4009/Lのラクトビオン酸を蓄積した.いずれの場合も,原料の加水分解や副生成物の生成を伴わず,収率は100%であった.得られた発酵培養液上清にエタノールを加えエタノール濃度75%とすることで,純度100%のLAが収率98%で得られた.

著者

橋本 博之 山下 亜希子 藤田 孝輝 岡田 正通 森 茂治 園山 慶 北畑 寿美雄

出版者

日本応用糖質科学会

雑誌

Journal of Applied Glycoscience (ISSN:13447882)

巻号頁・発行日

vol.51, no.2, pp.169-176, 2004 (Released:2008-03-24)

参考文献数

42

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Oligosaccharides with α-D-galactosidic linkages, especially the positional isomers of α-galactobiose (α-Gal 2), participate in such biological processes as immunity and pathogen-to-cell adhesion on the basis of molecular mechanisms for ligand-receptor interaction. α-Linked galactooligosaccharide (α-GOS) containing positional isomers of α-Gal 2 produced from galactose by the reverse reaction of α-galactosidase, is a potential food for the prevention of infections by pathogens, neutralization of toxins, and regulation of immune system. α-GOS (7.5 kg) was produced from galactose (18 kg; prepared from lactose hydrolyzate) by the reverse reaction of α-galactosidase from Aspergillus niger APC-9319. α-GOS contained 58% disaccharide, 28% trisaccharide and 14% oligosaccharides larger than trisaccharide. The disaccharide in α-GOS consisted largely of α-1,6-galactobiose (73%). α-GOS was stable at neutral and acidic pHs, and high temperatures. The sweetness of α-GOS was 25% of that of sucrose. The taste of α-GOS was mild and mellow. α-GOS reduced acid taste, bitterness, astringency, and harsh taste without spoiling the original taste in various fruit juices, and emphasized the original flavor of food materials in various cooked foods. α-GOS was not hydrolyzed in an in vitro digestion method. In oral tolerance tests of α-GOS, the blood glucose level did not change at all. α-GOS had a strong selective growth activity for Bifidobacterium sp. and Clostridium butyricum. An α-GOS intake in rats made the contents of lactate and n-butyrate in the cecum higher, and pH in the cecum lower than for a control intake. The viable count of Candida albicans in cecum and colon of mouse was decreased by an α-GOS intake more than by a raffinose intake. Therapeutic oral treatment with α-GOS had a potent inhibitory effect on adjuvant arthritis in Wister rats and on type II collagen-induced arthritis in DBA/1 J mice. Dietary α-GOS ameliorated allergic airway eosinophilia at least partly via post-absorptive mechanisms in Brown Norway rats.