- 著者
- 友田 正司/加藤 祥子
- 出版者
- 慶應義塾大学
- 雑誌
- 共立薬科大学研究年報 (ISSN:04529731)
- 巻号頁・発行日
- vol.11, pp.6-9, 1966-05-20
The water-soluble substances of the tuberous roots of Ophiopogon japonicus Ker-Gawler var. genuinus Maximowicz were obtained in 92% yield of the dry crude drug. The water-extract was fractionated by the chromatography on a column of charcoal-Celite, and D-glucose, D-fructose, sucrose and the other five oligosaccharide fractions were obtained. The carbohydrates have been examined by the use of paper partition chromatography and gas-liquid chromatography. D-Glucose, D-fructose and sucrose were identified respectively as D-glucose benzylphenylhydrazone, D-fructose methylphenylosazone, and sucrose octaacetate. The obtained all oligosaccharides were non-reducing glucofructan. The results of quantitative determinations of the monosaccharides and oligosaccharides showed that the water-extract contains 9.1% of D-glucose, 6.7% of D-fructose, 4.8% of sucrose and 56.7% of the other oligosaccharides.
1 0 0 0 OA バクモンドウの水溶性糖質(第 2 報) : 3 種の少糖類の精製, 性質および構造
- 著者
- 友田 正司/加藤 祥子
- 出版者
- 慶應義塾大学
- 雑誌
- 共立薬科大学研究年報 (ISSN:04529731)
- 巻号頁・発行日
- vol.13, pp.35-39, 1968-10-20
The main oligosaccharide fraction obtained from the water extract of the tuberous roots of Ophiopogon japonicus K_<ER>-G_<AWLER> var. genuinus M_<AXIMOWICZ> was purified by the gel filtration on Sephadex G-25,and separated into three oligosaccharides. They were a heptasaccharide composed of one glucose unit and six fructose units, a hexasaccharide composed of one glucose unit and five fructose units, and a pentasaccharide composed of one glucose unit and four fructose units. Methylation and periodate oxidation studies showed that the each oilgosaccharide possesses the structure consisted of a chain of 2→1 linked D-fructofuranose units having a D-glucopyranose residue, joined by a type of sucrose bond, on the end.