著者

新免 輝男 富永 義人 田沢 仁

出版者

一般社団法人 日本生物物理学会

雑誌

生物物理 (ISSN:05824052)

巻号頁・発行日

vol.25, no.1, pp.11-16, 1985-01-25 (Released:2009-05-25)

参考文献数

32

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Two types of demembranated cell models have been established using Characeae internodal cells; the tonoplast-free cell model and the plasmalemma-permeabilized cell model. In these models, both ATP and Mg2+ were essential for the maintenance of cytoplasmic streaming. Ca2+ inhibited the streaming in both models. However, the Ca2+-sensitivity of the streaming in the plasmalemma-permeabilized model was 1000 times higher than the tonoplast-free model, suggesting that some Ca2+-sensitizing component was dispersed or disorganized by the disintegration of the tonoplast. Using the actin bundles present in the tonoplast-free cells, the streaming was reconstituted with the endoplasm from other Characeae cells or the rabbit skeletal muscle, scallop or Physarum myosin. Ca2+ accelearated the streaming reconstituted with scallop myosin but inhibited that reconstituted with Physarum myosin. These opposite effects of Ca2+ on reconstituted streamings agree with the effects of Ca2+ on the respective actomyosin ATPases.